LOCUS       SUMO-HyperTEV60        6281 bp DNA     circular SYN 09-FEB-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 11, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6281
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    208..226
                     /label=T7
                     /note="/vntifkey=21"
     protein_bind    227..251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             265..287
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             301..330
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     misc_feature    337..630
                     /label=SUMO (Smt3)
     misc_feature    637..1299
                     /label=HyperTEV60
     primer_bind     complement(1461..1479)
                     /label=T7-Term
     terminator      1475..1522
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1558..2010
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    complement(2101..2910)
                     /label=KanR2
                     /note="/vntifkey=21"
     misc_feature    complement(3015..3629)
                     /label=PBR322 Origin
                     /label=PBR322_Origin
                     /note="/vntifkey=21"
     misc_feature    3799..3937
                     /label=bom
                     /note="basis of mobility region from pBR322"
     misc_feature    complement(4038..4228)
                     /label=ROP
                     /note="/vntifkey=21"
     misc_feature    complement(4699..4972)
                     /label=tet
                     /note="/vntifkey=21"
     misc_feature    complement(5030..6113)
                     /label=lacI
                     /note="/vntifkey=21"
     promoter        complement(6105..6181)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
       61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      121 ataggcgcag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      181 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
      241 ataacaattc cccttagaaa taattttgtt taactttaag aaggagatat acatatgggc
      301 catcaccatc accatcacca tcaccatcac ggcagcatga gcgatagcga agtgaaccag
      361 gaagcgaaac cggaagtgaa accggaagtg aaaccggaaa cccatattaa cctgaaagtg
      421 agcgatggca gcagcgaaat tttttttaaa attaaaaaaa ccaccccgct gcgtcgtctg
      481 atggaagcgt ttgcgaaacg tcagggcaaa gaaatggata gcctgcgttt tctgtatgat
      541 ggcattcgta ttcaggcgga tcagaccccg gaagatctgg atatggaaga taacgatatt
      601 attgaagcgc atcgtgaaca gattggcggc ggatccgcgg aaagcgcggc gccgggcccg
      661 cgcgattata acccgattag cgataccatt gttctgctga ccaataccag cgatggctat
      721 agcattagcc tgtatggcat tggctttggc ccgctgatta ttaccaacgc gcacctgttt
      781 cgccgcaaca acggcaccct gaccattacc agcaaacatg gcacctttac cattagcaac
      841 accaccaccc tgaaactgca tctgatcgaa ggccgcgatc tggtgctgat tgaaatgccg
      901 aaagattttc cgccgtttcc gaccaacctg gtgtttcgtg aaccggtggt gggcgaagaa
      961 attgtgctgg tgacccgcaa ctttcagacc aaaaccccga ccagcgaagt gagcgatgtg
     1021 agcaccacct atccgagctc cgatggcgtg ttttggaaac attggattcc gacgaaagat
     1081 ggccagtgcg gcagcccgat ggtgagcgtg accgatggca gcattgtggg cattcatagc
     1141 gcgagcaact ttaccaacac caacaactat tttaccgcgg tgccgccgga ttttatgcgc
     1201 ctgctgaccg atccgagcct gcagaaatgg gtgagcggct ggagcctgaa cagcgatagc
     1261 gtggaatggg gcggccataa agtgtttatg gataaaccgt aataaggatc catgggtacc
     1321 ctcgagtgaa gacaagctta ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca
     1381 acttagtcga gcaccaccac caccaccact gagtccggct gctaacaaag cccgaaagga
     1441 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     1501 acgggtcttg aggggttttt tgctgaaagg aggaactata ccggattggc gaatgggacg
     1561 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     1621 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttc tcgccacgtt
     1681 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     1741 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtggccatcg
     1801 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     1861 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     1921 atttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     1981 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     2041 aacccctatt gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc
     2101 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
     2161 accatatttt tgaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     2221 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     2281 attaatttcc cctcgtcaaa aaaaggttat caagtgagaa atcaccatga gtgacgactg
     2341 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc
     2401 cattacgctc gtcatcaaaa tcactcgcac aaccaaaccg ttattcattc gtgattgcgc
     2461 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg
     2521 caaccggcgc aggaacactg ccagcgcatc aacaattttt cacctgaatc aggatattct
     2581 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     2641 ggagtacgga taaaatgctt gatggtcgga agaggcataa attcgtcagc cagtttagtc
     2701 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     2761 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc cgacattatc
     2821 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga
     2881 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtagca
     2941 gacagtttta ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3001 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3061 ctgctgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3121 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     3181 ctagtgtagc ctagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3241 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3301 tggactcaag acgatagtac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3361 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3421 atgagaaagc gccacgcttc ccgaaggaga aaggcggaca ggtatccggt aagcggcagg
     3481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3541 cctgtcgggt ttcgccacct ctgacttgag cgcgattttt gtgatgctcg tcaggggggc
     3601 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3661 cttttgctca catgttcttt cctgcgttat cccctgattt gtggataacc gtattaccgc
     3721 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     3781 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcattgtg cggtatttca
     3841 caccgcaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     3901 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caaacccgct
     3961 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     4021 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     4081 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     4141 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
     4201 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga ttctgttcat
     4261 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
     4321 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggac
     4381 cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca
     4441 cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac
     4501 ttccggtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg
     4561 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct
     4621 gctaaccagt aagcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat
     4681 gcgcacccgt ggggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt
     4741 ggcgggacca gtgacgaagc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac
     4801 aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct
     4861 gccggcacct gtcctacgag ttgcatataa agaagacagt cataagtgcg gcgacgatag
     4921 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
     4981 gagatcccgg tgcctaatga gtgagctaac ttaattaatt gcgttgcgct cactgcccgc
     5041 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     5101 aggcggtttg cgtattgggc gccagggtgg tttttctttt accagtgaga cgggcaacag
     5161 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
     5221 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataca tgagctgtct
     5281 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta
     5341 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtggaacga
     5401 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
     5461 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
     5521 gagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
     5581 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
     5641 gatgggtgct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc
     5701 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc
     5761 gcgagaagat tgtgcccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
     5821 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
     5881 gcgcgtgcag ggccagactg gagtggcaac gccaatcagc aacgactgtt tgcccgccag
     5941 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     6001 ccgcgttttc gcagaaacgt ggctggcctg ttcaccacgc gggaaacggt ctgataagag
     6061 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     6121 tgactctctt ccgggcgcta tcatgccata ccgcgaaggt tttgcgccat tcgatggtgt
     6181 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     6241 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat g
//
