LOCUS       myc:Skp1(dC111):        7528 bp    DNA     circular SYN 14-OCT-2024
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-15665061|
COMMENT     VNTDBDATE|-15665061|
COMMENT     LSOWNER|
COMMENT     VNTNAME|myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA|
COMMENT     VNTAUTHORNAME|anzeve|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="pcDNA3"
                     /mol_type="other DNA"
                     /vntifkey="98"
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(3081..3098)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(3082..3099)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(3118..3135)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      3121..3348
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      3411..3717
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(3831..3851)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(3837..4053)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        3849..4118
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      4017..4094
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    4079..4098
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             4233..5027
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            4236..5024
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      5204..5323
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    5292..5311
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(5367..5385)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(5384..5406)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(5420..5449)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(5758..6377)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(6532..7392)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(6532..7392)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(7434..7462)
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_feature    1373..3025
                     /vntifkey="21"
                     /label=luc2
     misc_feature    3035..3061
                     /vntifkey="21"
                     /label=HA\tag
     misc_feature    3026..3034
                     /vntifkey="21"
                     /label=gsg\linker
     misc_feature    1334..1354
                     /vntifkey="21"
                     /label=TEVs
     misc_feature    1355..1372
                     /vntifkey="21"
                     /label=gs6
     misc_feature    1316..1333
                     /vntifkey="21"
                     /label=gs6
     misc_feature    983..1315
                     /vntifkey="21"
                     /label=Skp1(dc111)
     misc_feature    953..982
                     /vntifkey="21"
                     /label=myc\tag
BASE COUNT     1749 a      2040 c      1989 g      1750 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tggagcagaa 
      961 gctgatcagc gaggaggacc tgcccagcat caagctgcag agcagcgacg gcgagatctt 
     1021 cgaggtggac gtggagatcg ccaagcagag cgtgaccatc aagaccatgc tggaggacct 
     1081 gggcatggac gacgagggcg acgacgaccc cgtgcccctg cccaacgtga acgccgccat 
     1141 cctgaagaag gtgatccagt ggtgcaccca ccacaaggac gaccctcctc ctcctgagga 
     1201 cgacgagaac aaggagaaga ggaccgacga catccccgtg tgggaccagg agttcctgaa 
     1261 ggtggaccag ggcaccctgt tcgagctgat cctggccgcc aactacctgg acatcggcgg 
     1321 ctctggcggc ggcgaaaatt tgtatttcca gagtggcggc tccggaggct ctgaagatgc 
     1381 caaaaacatt aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga 
     1441 gcagctgcac aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga 
     1501 cgcacatatc gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc 
     1561 agaagctatg aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa 
     1621 tagcttgcag ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc 
     1681 agctaacgac atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac 
     1741 cgtcgtattc gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc 
     1801 gatcatacaa aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat 
     1861 gtacaccttc gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga 
     1921 gagcttcgac cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt 
     1981 gcccaagggc gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga 
     2041 ccccatcttc ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca 
     2101 ccacggcttc ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct 
     2161 catgtaccgc ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc 
     2221 tgccctgctg gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta 
     2281 cgacctaagc aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg 
     2341 tgaggccgtg gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga 
     2401 aacaaccagc gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa 
     2461 ggtggtgccc ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt 
     2521 gaaccagcgc ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa 
     2581 ccccgaggct acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc 
     2641 ctactgggac gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata 
     2701 caagggctac caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat 
     2761 cttcgacgcc ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt 
     2821 cgtcgtgctg gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag 
     2881 ccaggttaca accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa 
     2941 aggactgacc ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa 
     3001 gggcggcaag atcgccgtga atagtggttc tggataccca tacgatgttc cagattacgc 
     3061 ttaatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct 
     3121 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 
     3181 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 
     3241 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 
     3301 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 
     3361 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 
     3421 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 
     3481 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 
     3541 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 
     3601 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 
     3661 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 
     3721 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt 
     3781 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 
     3841 tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc 
     3901 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 
     3961 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 
     4021 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 
     4081 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 
     4141 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 
     4201 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 
     4261 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 
     4321 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 
     4381 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc 
     4441 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 
     4501 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 
     4561 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 
     4621 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 
     4681 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 
     4741 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg 
     4801 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 
     4861 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 
     4921 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 
     4981 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 
     5041 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 
     5101 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 
     5161 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 
     5221 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 
     5281 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc 
     5341 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 
     5401 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 
     5461 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 
     5521 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 
     5581 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 
     5641 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 
     5701 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 
     5761 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 
     5821 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 
     5881 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 
     5941 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta 
     6001 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 
     6061 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 
     6121 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 
     6181 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 
     6241 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 
     6301 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 
     6361 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 
     6421 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 
     6481 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 
     6541 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 
     6601 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 
     6661 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 
     6721 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 
     6781 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 
     6841 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 
     6901 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 
     6961 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 
     7021 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 
     7081 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 
     7141 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 
     7201 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 
     7261 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 
     7321 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 
     7381 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 
     7441 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 
     7501 atttccccga aaagtgccac ctgacgtc 
//
