LOCUS       F-box:gs10:FRB:H        5971 bp    DNA     circular SYN 14-OCT-2024
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-15665064|
COMMENT     VNTDBDATE|-15665064|
COMMENT     LSOWNER|
COMMENT     VNTNAME|F-box:gs10:FRB:HA|
COMMENT     VNTAUTHORNAME|anzeve|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="pcDNA3"
                     /mol_type="other DNA"
                     /vntifkey="98"
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(1524..1541)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(1525..1542)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(1561..1578)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      1564..1791
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      1854..2160
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(2274..2294)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(2280..2496)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        2292..2561
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      2460..2537
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    2522..2541
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             2676..3470
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            2679..3467
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      3647..3766
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    3735..3754
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(3810..3828)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(3827..3849)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(3863..3892)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(4201..4820)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(4975..5835)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(4975..5835)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(5877..5905)
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_feature    1160..1189
                     /vntifkey="21"
                     /label=gs10\linker
     misc_feature    953..1159
                     /vntifkey="21"
                     /label=Skp2\F-box
     misc_feature    1190..1477
                     /vntifkey="21"
                     /label=FRB
     misc_feature    1478..1504
                     /vntifkey="21"
                     /label=HA\tag
BASE COUNT     1371 a      1550 c      1559 g      1491 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgagggagaa 
      961 cttccccggc gtgagctggg acagcctgcc cgacgagctg ctgctgggca tcttcagctg 
     1021 cctgtgcctg cccgagctgc tgaaggtgag cggcgtgtgc aagaggtggt acaggctggc 
     1081 cagcgacgag agcctgtggc agaccctgga cctgaccggc aagaacctgc accccgacgt 
     1141 gaccggcagg ctgctgagcg gcggctctgg cggcggctcc ggaggctctg cttctagaat 
     1201 cctctggcat gagatgtggc atgaaggcct ggaagaggca tctcgtttgt actttgggga 
     1261 aaggaacgtg aaaggcatgt ttgaggtgct ggagcccttg catgctatga tggaacgggg 
     1321 cccccagact ctgaaggaaa catcctttaa tcaggcctat ggtcgagatt taatggaggc 
     1381 ccaagagtgg tgcaggaagt acatgaaatc agggaatgtc aaggacctcc tccaagcctg 
     1441 ggacctctat tatcatgtgt tccgacgaat ctcaaagtac ccatacgatg ttccagatta 
     1501 cgcttaatct agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag 
     1561 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 
     1621 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 
     1681 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 
     1741 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg 
     1801 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa 
     1861 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     1921 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     1981 ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     2041 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     2101 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     2161 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct 
     2221 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 
     2281 gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca 
     2341 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 
     2401 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 
     2461 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 
     2521 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 
     2581 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 
     2641 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 
     2701 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 
     2761 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 
     2821 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt 
     2881 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 
     2941 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 
     3001 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 
     3061 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 
     3121 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 
     3181 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg 
     3241 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 
     3301 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 
     3361 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 
     3421 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct 
     3481 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac 
     3541 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 
     3601 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc 
     3661 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 
     3721 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc 
     3781 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 
     3841 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 
     3901 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 
     3961 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 
     4021 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 
     4081 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
     4141 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     4201 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     4261 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     4321 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     4381 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt 
     4441 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     4501 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     4561 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     4621 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 
     4681 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     4741 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     4801 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
     4861 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     4921 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     4981 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     5041 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     5101 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     5161 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     5221 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     5281 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     5341 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     5401 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     5461 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
     5521 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
     5581 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
     5641 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
     5701 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
     5761 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
     5821 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 
     5881 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
     5941 cacatttccc cgaaaagtgc cacctgacgt c 
//
