LOCUS       myc:FRB:gs10:Cul        6592 bp    DNA     circular SYN 14-OCT-2024
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-15665048|
COMMENT     VNTDBDATE|-15665048|
COMMENT     LSOWNER|
COMMENT     VNTNAME|myc:FRB:gs10:Cul1(dC501)|
COMMENT     VNTAUTHORNAME|anzeve|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="pcDNA3"
                     /mol_type="other DNA"
                     /vntifkey="98"
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(2145..2162)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(2146..2163)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(2182..2199)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      2185..2412
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      2475..2781
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(2895..2915)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(2901..3117)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        2913..3182
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      3081..3158
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    3143..3162
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             3297..4091
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            3300..4088
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      4268..4387
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    4356..4375
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(4431..4449)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(4448..4470)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(4484..4513)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(4822..5441)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(5596..6456)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(5596..6456)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(6498..6526)
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_feature    953..982
                     /vntifkey="21"
                     /label=myc\tag
     misc_feature    1271..1300
                     /vntifkey="21"
                     /label=gs10\linker
     misc_feature    1301..2125
                     /vntifkey="21"
                     /label=Cul1(dC501)
     misc_feature    983..1270
                     /vntifkey="21"
                     /label=FRB
BASE COUNT     1603 a      1657 c      1676 g      1656 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tggagcagaa 
      961 gctgatcagc gaggaggacc tggcttctag aatcctctgg catgagatgt ggcatgaagg 
     1021 cctggaagag gcatctcgtt tgtactttgg ggaaaggaac gtgaaaggca tgtttgaggt 
     1081 gctggagccc ttgcatgcta tgatggaacg gggcccccag actctgaagg aaacatcctt 
     1141 taatcaggcc tatggtcgag atttaatgga ggcccaagag tggtgcagga agtacatgaa 
     1201 atcagggaat gtcaaggacc tcctccaagc ctgggacctc tattatcatg tgttccgacg 
     1261 aatctcaaag ggcggctctg gcggcggctc cggaggctct tctaaacttc agcgcatgtt 
     1321 tcaagacatt ggcgtgagca aagatctgaa cgagcaattc aaaaagcact tgacaaactc 
     1381 agaaccccta gacttggatt tcagcattca agtgctgagc tccgggtcct ggcccttcca 
     1441 gcagtcttgt acatttgcct tgccgtcaga gttggaacgt agttatcagc gattcacagc 
     1501 tttctacgcc agccgccaca gtggccgaaa attgacgtgg ttatatcagt tgtctaaagg 
     1561 agaattggta actaactgct tcaaaaacag atatactttg caggcgtcga cattccagat 
     1621 ggctatcctg cttcagtaca acacggaaga tgcctacact gtgcagcagc tgaccgacag 
     1681 cactcaaatt aaaatggaca ttttggcgca agttttacag attttattaa agtcgaagct 
     1741 attggtcttg gaagatgaaa atgcaaatgt tgatgaggtg gaattgaagc cagatacctt 
     1801 aataaaatta tatcttggtt ataaaaataa gaaattaagg gttaacatca atgtgccaat 
     1861 gaaaaccgaa cagaagcagg aacaagaaac cacacacaaa aacatcgagg aagaccgcaa 
     1921 actactgatt caggcggcca tcgtgagaat catgaagatg aggaaggttc tgaaacacca 
     1981 gcagttactt ggcgaggtcc tcactcagct gtcctccagg ttcaaacctc gagtccctgt 
     2041 gatcaagaaa tgcattgaca ttctaattga gaaagaatat ttggagcgag tggatggtga 
     2101 aaaggacacc tacagttact tggcttaatc tagagggccc tattctatag tgtcacctaa 
     2161 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt 
     2221 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 
     2281 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 
     2341 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 
     2401 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg 
     2461 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 
     2521 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 
     2581 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg 
     2641 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 
     2701 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 
     2761 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 
     2821 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
     2881 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg 
     2941 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 
     3001 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 
     3061 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     3121 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 
     3181 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 
     3241 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga 
     3301 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 
     3361 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 
     3421 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg 
     3481 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 
     3541 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 
     3601 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 
     3661 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 
     3721 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 
     3781 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg 
     3841 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 
     3901 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 
     3961 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 
     4021 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 
     4081 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 
     4141 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 
     4201 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 
     4261 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 
     4321 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 
     4381 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 
     4441 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 
     4501 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 
     4561 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 
     4621 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 
     4681 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
     4741 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
     4801 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
     4861 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
     4921 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
     4981 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg 
     5041 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     5101 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     5161 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     5221 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 
     5281 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
     5341 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
     5401 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
     5461 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
     5521 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
     5581 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 
     5641 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 
     5701 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 
     5761 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 
     5821 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
     5881 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 
     5941 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 
     6001 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 
     6061 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 
     6121 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
     6181 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 
     6241 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 
     6301 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 
     6361 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 
     6421 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
     6481 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 
     6541 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc 
//
