LOCUS       FRB:GSlinker:SSD        6274 bp    DNA     circular SYN 14-OCT-2024
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-15665056|
COMMENT     VNTDBDATE|-15665056|
COMMENT     LSOWNER|
COMMENT     VNTNAME|FRB:GSlinker:SSD|
COMMENT     VNTAUTHORNAME|anzeve|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="pcDNA3"
                     /mol_type="other DNA"
                     /vntifkey="98"
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(1827..1844)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(1828..1845)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(1864..1881)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      1867..2094
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      2157..2463
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(2577..2597)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(2583..2799)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        2595..2864
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      2763..2840
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    2825..2844
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             2979..3773
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            2982..3770
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      3950..4069
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    4038..4057
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(4113..4131)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(4130..4152)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(4166..4195)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(4504..5123)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(5278..6138)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(5278..6138)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(6180..6208)
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_feature    1475..1807
                     /vntifkey="21"
                     /label=Skp1(dc111)
     misc_feature    1322..1444
                     /vntifkey="21"
                     /label=SOCSbox
     misc_feature    983..1270
                     /vntifkey="21"
                     /label=FRB
     misc_feature    953..982
                     /vntifkey="21"
                     /label=myc\tag
     misc_feature    1445..1474
                     /vntifkey="21"
                     /label=gs10v3
     misc_feature    1271..1321
                     /vntifkey="21"
                     /label=GS\linker
BASE COUNT     1449 a      1647 c      1657 g      1521 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tggagcagaa 
      961 gctgatcagc gaggaggacc tggcttctag aatcctctgg catgagatgt ggcatgaagg 
     1021 cctggaagag gcatctcgtt tgtactttgg ggaaaggaac gtgaaaggca tgtttgaggt 
     1081 gctggagccc ttgcatgcta tgatggaacg gggcccccag actctgaagg aaacatcctt 
     1141 taatcaggcc tatggtcgag atttaatgga ggcccaagag tggtgcagga agtacatgaa 
     1201 atcagggaat gtcaaggacc tcctccaagc ctgggacctc tattatcatg tgttccgacg 
     1261 aatctcaaag ggcggctctg gcggcggctc cggaggctct ggcggcggct ccggaggctc 
     1321 taccagcgcc cccagcctgc agcacctgtg caggctgacc atcaacaagt gcaccggcgc 
     1381 catctggggc ctgcccctgc ccaccaggct gaaggactac ctggaggagt acaagttcca 
     1441 ggtgggggga tccggaggcg gatctggcgg gagccccagc atcaagctgc agagcagcga 
     1501 cggcgagatc ttcgaggtgg acgtggagat cgccaagcag agcgtgacca tcaagaccat 
     1561 gctggaggac ctgggcatgg acgacgaggg cgacgacgac cccgtgcccc tgcccaacgt 
     1621 gaacgccgcc atcctgaaga aggtgatcca gtggtgcacc caccacaagg acgaccctcc 
     1681 tcctcctgag gacgacgaga acaaggagaa gaggaccgac gacatccccg tgtgggacca 
     1741 ggagttcctg aaggtggacc agggcaccct gttcgagctg atcctggccg ccaactacct 
     1801 ggacatctaa tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat 
     1861 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 
     1921 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 
     1981 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 
     2041 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 
     2101 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat 
     2161 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 
     2221 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 
     2281 aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc 
     2341 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 
     2401 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 
     2461 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg 
     2521 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa 
     2581 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa 
     2641 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 
     2701 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 
     2761 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     2821 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 
     2881 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 
     2941 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 
     3001 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 
     3061 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 
     3121 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 
     3181 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 
     3241 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 
     3301 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 
     3361 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 
     3421 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 
     3481 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 
     3541 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 
     3601 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 
     3661 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 
     3721 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 
     3781 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 
     3841 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 
     3901 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 
     3961 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 
     4021 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 
     4081 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 
     4141 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 
     4201 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 
     4261 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 
     4321 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 
     4381 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 
     4441 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 
     4501 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 
     4561 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 
     4621 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 
     4681 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc 
     4741 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 
     4801 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 
     4861 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 
     4921 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 
     4981 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 
     5041 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
     5101 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 
     5161 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 
     5221 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 
     5281 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 
     5341 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 
     5401 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 
     5461 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 
     5521 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 
     5581 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 
     5641 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 
     5701 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 
     5761 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 
     5821 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 
     5881 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 
     5941 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 
     6001 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 
     6061 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 
     6121 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 
     6181 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 
     6241 gcgcacattt ccccgaaaag tgccacctga cgtc 
//
