LOCUS       YAP/TAZ-TRE-TdTomato_re 8789 bp ds-DNA     circular     14-OCT-2024
DEFINITION  This plasmid is a YAP/TAZ pathway reporter. It has a
            YAP/TAZ-responsive synthetic promoter driving the expression of
            mStrawberry-pGK-BSD.
KEYWORDS    "accession:addgene_158682_309773"
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported from database: AddGene Entry: YAP/TAZ-TRE-mStrawberry
            reporter URL: https://www.addgene.org/browse/sequence/309773/
            GENERAL Depositing Lab: Ravid Straussman Publication: Kaufman et al
            Nat Commun. 2022 May 18;13(1):2725. doi:
            10.1038/s41467-022-30008-0. Purpose: This plasmid is a YAP/TAZ
            pathway reporter. It has a YAP/TAZ-responsive synthetic promoter
            driving the expression of mStrawberry-pGK-BSD BACKBONE Backbone
            size w/o insert (bp): 7728 Selectable markers: Blasticidin Vector
            backbone: pLKO.1 Vector type: Mammalian Expression, Lentiviral
            CLONING INFORMATION Cloning method: Gibson Cloning GROWTH IN
            BACTERIA Bacterial Resistance(s): Ampicillin, 100 μg/mL Copy
            number: High Copy Growth Strain(s): NEB Stable Growth Temperature:
            37°C GENE/INSERT Gene/Insert name: mStrawberry Insert Size (bp):
            341 Species: H. sapiens (human) TERMS AND LICENSES
            Academic/Nonprofit Terms:  Industry Terms:
FEATURES             Location/Qualifiers
     protein_bind    23..38
                     /label="2xGTIIC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with high affinity for TEF-1 family (TEAD) proteins (Mahoney et al., 2005)"
                     /bound_moiety="TEF-1 family (TEAD) proteins"
     protein_bind    55..70
                     /label="2xGTIIC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with high affinity for TEF-1 family (TEAD) proteins (Mahoney et al., 2005)"
                     /bound_moiety="TEF-1 family (TEAD) proteins"
     protein_bind    109..124
                     /label="2xGTIIC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with high affinity for TEF-1 family (TEAD) proteins (Mahoney et al., 2005)"
                     /bound_moiety="TEF-1 family (TEAD) proteins"
     protein_bind    141..156
                     /label="2xGTIIC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with high affinity for TEF-1 family (TEAD) proteins (Mahoney et al., 2005)"
                     /bound_moiety="TEF-1 family (TEAD) proteins"
     primer_bind     182..198
                     /label="pBluescriptKS"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="For pBluescript vector"
     primer_bind     183..199
                     /label="KS primer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="common sequencing primer, one of multiple similar variants"
     primer          183..199
                     /label="KS Bluescript (Genewiz)"
                     /note="sequence: cgaggtcgacggtatcg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    321..1799
                     /label="gBlock: tdTomato"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(323..340)
                     /label="JH_43"
                     /note="sequence: gggtcgctttaccaacag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             341..1771
                     /label="tdTomato"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHLFLYGMDELYK*"
     primer          1780..1799
                     /label="JH_39"
                     /note="sequence: gatcttgagacaaatggcag"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1807..1828)
                     /label="CPP/CTS Sequencing (Genewiz)"
                     /note="sequence: cccttttcttttaaaattgtgg"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1813..1930
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="central polypurine tract and central termination sequence of HIV-1"
     promoter        1989..2489
                     /label="hPGK promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2408..2426
                     /label="hPGK-F"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Human PGK promoter, forward primer"
     CDS             2501..2896
                     /label="BSD"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="confers resistance to blasticidin"
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer          2503..2522
                     /label="JH_42"
                     /note="sequence: ggccaagcctttgtctcaag"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2932..3520
                     /label="WPRE"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="woodchuck hepatitis virus posttranscriptional regulatory element"
     primer_bind     complement(2985..3005)
                     /label="WPRE-R"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="WPRE, reverse primer"
     CDS             complement(3403..3414)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /product="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     LTR             3592..3825
                     /label="3' LTR (Delta-U3)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="self-inactivating 3' long terminal repeat (LTR) fromHIV-1"
     polyA_signal    3903..4024
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3940..3959)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="SV40 polyA, reverse primer"
     primer_bind     3994..4013
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4064..4199
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="SV40 origin of replication"
     primer_bind     4126..4145
                     /label="SV40pro-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4219..4238)
                     /label="T7"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="T7 promoter, forward primer"
     promoter        complement(4220..4238)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4248..4264)
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="common sequencing primer, one of multiple similar variants"
     primer_bind     complement(4248..4265)
                     /label="M13 Forward"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward"
     primer_bind     complement(4257..4279)
                     /label="M13/pUC Forward"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="In lacZ gene"
     rep_origin      4406..4861
                     /label="f1 ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
                     /direction="RIGHT"
     primer_bind     complement(4493..4512)
                     /label="F1ori-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="F1 origin, reverse primer"
     primer_bind     4703..4724
                     /label="F1ori-F"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="F1 origin, forward primer"
     primer          4876..4891
                     /label="JH_35"
                     /note="sequence: ggggaaatgtgcgcgg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        4887..4991
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /gene="bla"
     CDS             4992..5852
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"
                     /gene="bla"
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     primer_bind     complement(5210..5229)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6023..6611
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /direction="RIGHT"
     primer_bind     6512..6531
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pBR322 origin, forward primer"
     primer_bind     6765..6782
                     /label="L4440"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="L4440 vector, forward primer"
     protein_bind    6899..6920
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="CAP binding activates transcription in the presence of cAMP."
                     /bound_moiety="E. coli catabolite activator protein"
     promoter        6935..6965
                     /label="lac promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6973..6989
                     /label="lac operator"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /bound_moiety="lac repressor encoded by lacI"
     primer_bind     6978..7000
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="In lacZ gene"
     primer_bind     6997..7013
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6997..7013
                     /label="M13 rev"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="common sequencing primer, one of multiple similar variants"
     primer_bind     7032..7052
                     /label="T3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="T3 promoter, forward primer"
     promoter        7034..7052
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7080..7306
                     /label="RSV promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7307..7487
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7534..7659
                     /label="HIV-1 Psi"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="packaging signal of human immunodeficiency virus type 1"
     misc_feature    8152..8385
                     /label="RRE"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm."
     CDS             8570..8614
                     /label="gp41 peptide"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)"
                     /translation="KNEQELLELDKWASL"
     primer          8668..8689
                     /label="JH-QRT_1"
                     /note="sequence: GATAGTAGGAGGCTTGGTAGGT"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(8748..8769)
                     /label="JH-QRT_2"
                     /note="sequence: AGGTGGGTCTGAAACGATAATG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 cggccagtgc caagttgaga cacattccac acattccact gcaagcttga gacacattcc
       61 acacattcca ctgcaagctt ctagcccggc cagtgccaag ttgagacaca ttccacacat
      121 tccactgcaa gcttgagaca cattccacac attccactgc aagcttctag agatctgcag
      181 gtcgaggtcg acggtatcga taagcttggg ggtgggcgcc ggggggacct taaagcctct
      241 gccccccaag gagcccttcc cagacagccg ccggcaccca ccgctccgtg ggacgatccc
      301 ccaagagctt ggcattccgg tactgttggt aaagcgaccc ATGGTAAGTA AGGGGGAGGA
      361 AGTTATCAAA GAATTCATGA GATTTAAAGT AAGGATGGAG GGTTCTATGA ACGGGCACGA
      421 ATTCGAAATA GAGGGGGAAG GCGAGGGCCG GCCCTACGAA GGGACGCAGA CGGCGAAACT
      481 TAAAGTGACG AAAGGGGGGC CGCTTCCGTT CGCTTGGGAC ATTCTTAGTC CTCAGTTCAT
      541 GTATGGCTCT AAGGCATACG TCAAACACCC TGCGGATATA CCTGATTACA AAAAGCTTAG
      601 CTTTCCCGAG GGTTTCAAAT GGGAGCGCGT AATGAATTTT GAGGATGGTG GGTTGGTGAC
      661 AGTCACCCAA GACTCCAGTT TGCAAGATGG TACCCTCATT TACAAGGTTA AAATGAGAGG
      721 TACTAACTTT CCCCCAGACG GTCCAGTTAT GCAGAAGAAA ACTATGGGTT GGGAAGCAAG
      781 CACCGAACGG CTTTATCCTA GAGACGGTGT GCTTAAGGGG GAGATACACC AGGCGCTTAA
      841 GCTGAAAGAC GGTGGACACT ATCTTGTTGA ATTCAAGACG ATATATATGG CAAAGAAGCC
      901 TGTGCAACTT CCTGGCTACT ACTACGTAGA CACTAAGCTT GACATCACAT CCCATAATGA
      961 AGATTACACT ATCGTTGAAC AGTATGAACG ATCAGAGGGG CGCCACCACC TTTTCTTGGG
     1021 TCATGGAACG GGCAGCACCG GATCTGGTTC TTCTGGTACA GCATCATCAG AAGATAATAA
     1081 CATGGCAGTA ATCAAAGAGT TTATGAGGTT TAAGGTCCGA ATGGAGGGAT CTATGAACGG
     1141 GCACGAATTT GAGATCGAAG GTGAGGGTGA GGGAAGGCCT TACGAAGGTA CACAAACAGC
     1201 AAAGCTGAAG GTGACCAAGG GTGGGCCACT CCCCTTCGCA TGGGACATTC TTTCACCACA
     1261 ATTTATGTAC GGTTCCAAGG CGTATGTGAA GCATCCAGCT GATATCCCGG ATTATAAAAA
     1321 GCTTTCCTTC CCAGAGGGCT TTAAGTGGGA GAGAGTTATG AACTTCGAAG ACGGCGGTCT
     1381 GGTCACAGTT ACACAGGATA GTAGTCTTCA GGATGGCACG TTGATCTACA AGGTTAAAAT
     1441 GAGGGGGACT AATTTTCCTC CTGATGGGCC TGTAATGCAA AAAAAAACTA TGGGTTGGGA
     1501 GGCCAGTACA GAACGCCTTT ATCCAAGGGA TGGAGTCCTG AAAGGTGAAA TACATCAGGC
     1561 GCTCAAGCTC AAAGACGGTG GACATTATTT GGTTGAGTTC AAGACGATTT ACATGGCCAA
     1621 GAAACCGGTT CAACTTCCAG GATACTACTA CGTAGATACT AAGCTGGACA TTACTTCTCA
     1681 TAATGAAGAT TATACCATCG TGGAACAATA CGAGCGATCT GAGGGACGGC ATCATTTGTT
     1741 TCTCTACGGT ATGGATGAAC TTTATAAGTA Agaattctag atcttgagac aaatggcagt
     1801 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     1861 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     1921 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg
     1981 ggcccgggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg
     2041 ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg
     2101 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg
     2161 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg
     2221 aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc
     2281 gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg
     2341 gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc
     2401 ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg
     2461 ttgaccgaat caccgacctc tctccccagg gggtaccacc atggccaagc ctttgtctca
     2521 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     2581 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     2641 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     2701 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     2761 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     2821 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     2881 ttatgtgtgg gagggcctgc agctgcagta gtaaagacgc gttaagtcga caatcaacct
     2941 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3001 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3061 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3121 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3181 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3241 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3301 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     3361 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     3421 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     3481 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     3541 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     3601 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     3661 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     3721 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     3781 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     3841 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     3901 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3961 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4021 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     4081 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4141 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4201 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     4261 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4321 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4381 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4441 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4501 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4561 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4621 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4681 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4741 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4801 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     4861 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4921 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4981 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5041 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5101 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5161 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5221 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5281 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5341 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5401 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5461 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5521 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5581 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5641 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5701 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5761 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5821 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5881 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5941 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6001 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6061 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6781 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6841 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6901 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6961 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7021 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     7081 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     7141 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     7201 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     7261 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     7321 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7381 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7441 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     7501 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     7561 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     7621 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     7681 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     7741 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     7801 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     7861 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     7921 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     7981 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8041 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8101 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8161 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8221 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     8281 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     8341 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     8401 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     8461 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     8521 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     8581 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     8641 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     8701 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     8761 gacccacctc ccaaccccga gggctagcc
//