LOCUS       Exported               12666 bp ds-DNA     circular SYN 24-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pLentiRfxCas13d-dead (rtTA-P2A-BSD)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12666)
  AUTHORS   Simon M
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 21, 2024 from SnapGene 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12666
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(161..1021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1022..1126)
                     /gene="bla"
                     /note="AmpR promoter"
     enhancer        1392..1771
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1773..1971
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1989..2169
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2216..2341
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2834..3067
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3594..3711
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3756..3776
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB4"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3821..3839
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3856..3874
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3892..3910
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3928..3946
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3963..3981
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3999..4017
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4029..4067
                     /note="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4132..4152
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             4162..7059
                     /codon_start=1
                     /note="dRfxCas13d"
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLAHWVVANNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFANKAVALEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             7069..7089
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             7099..7125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     misc_feature    7175..7763
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7646..7657)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     primer_bind     complement(7766..7782)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7828..8039
                     /note="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             8064..8768
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /note="rtTA3"
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     CDS             8778..8834
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8835..9233
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /note="BSD"
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             9625..9805
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9837..10061
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10107..10535
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        10549..10878
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      10729..10864
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        10926..10973
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             10992..11366
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11496..11617
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11666..11682)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11690..11706
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11714..11744)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    11759..11780
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(12068..12656)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
       61 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      121 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      181 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
      241 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
      301 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
      361 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
      421 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
      481 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
      541 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
      601 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
      661 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
      721 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
      781 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
      841 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
      901 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
      961 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1021 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1081 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1141 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     1201 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     1261 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     1321 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     1381 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     1441 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     1501 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     1561 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     1621 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     1681 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     1741 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     1801 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     1861 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     1921 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
     1981 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     2041 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     2101 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     2161 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     2221 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     2281 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     2341 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     2401 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     2461 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     2521 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     2581 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     2641 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     2701 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     2761 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     2821 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     2881 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     2941 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     3001 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     3061 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     3121 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     3181 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     3241 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     3301 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     3361 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     3421 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     3481 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     3541 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     3601 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     3661 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     3721 tacagggaca gcagagatcc agtttggtta attaacaact ttgtatagaa aagttggctc
     3781 cgaattcgcc cttcaggtcc gaggttctag acgagtttac tccctatcag tgatagagaa
     3841 cgatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
     3901 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
     3961 tatccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     4021 tatgtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc
     4081 gtcagatcgc aaagggcgaa ttcgacccaa gtttgtacag ccaccatgag ccccaagaag
     4141 aagagaaagg tggaggccag catcgaaaaa aaaaagtcct tcgccaaggg catgggcgtg
     4201 aagtccacac tcgtgtccgg ctccaaagtg tacatgacaa ccttcgccga aggcagcgac
     4261 gccaggctgg aaaagatcgt ggagggcgac agcatcagga gcgtgaatga gggcgaggcc
     4321 ttcagcgctg aaatggccga taaaaacgcc ggctataaga tcggcaacgc caaattcagc
     4381 catcctaagg gctacgccgt ggtggctaac aaccctctgt atacaggacc cgtccagcag
     4441 gatatgctcg gcctgaagga aactctggaa aagaggtact tcggcgagag cgctgatggc
     4501 aatgacaata tttgtatcca ggtgatccat aacatcctgg acattgaaaa aatcctcgcc
     4561 gaatacatta ccaacgccgc ctacgccgtc aacaatatct ccggcctgga taaggacatt
     4621 attggattcg gcaagttctc cacagtgtat acctacgacg aattcaaaga ccccgagcac
     4681 catagggccg ctttcaacaa taacgataag ctcatcaacg ccatcaaggc ccagtatgac
     4741 gagttcgaca acttcctcga taaccccaga ctcggctatt tcggccaggc ctttttcagc
     4801 aaggagggca gaaattacat catcaattac ggcaacgaat gctatgacat tctggccctc
     4861 ctgagcggac tggcgcactg ggtggtcgct aacaacgaag aagagtccag gatctccagg
     4921 acctggctct acaacctcga taagaacctc gacaacgaat acatctccac cctcaactac
     4981 ctctacgaca ggatcaccaa tgagctgacc aactccttct ccaagaactc cgccgccaac
     5041 gtgaactata ttgccgaaac tctgggaatc aaccctgccg aattcgccga acaatatttc
     5101 agattcagca ttatgaaaga gcagaaaaac ctcggattca atatcaccaa gctcagggaa
     5161 gtgatgctgg acaggaagga tatgtccgag atcaggaaaa atcataaggt gttcgactcc
     5221 atcaggacca aggtctacac catgatggac tttgtgattt ataggtatta catcgaagag
     5281 gatgccaagg tggctgccgc caataagtcc ctccccgata atgagaagtc cctgagcgag
     5341 aaggatatct ttgtgattaa cctgaggggc tccttcaacg acgaccagaa ggatgccctc
     5401 tactacgatg aagctaatag aatttggaga aagctcgaaa atatcatgca caacatcaag
     5461 gaatttaggg gaaacaagac aagagagtat aagaagaagg acgcccctag actgcccaga
     5521 atcctgcccg ctggccgtga tgtttccgcc ttcagcaaac tcatgtatgc cctgaccatg
     5581 ttcctggatg gcaaggagat caacgacctc ctgaccaccc tgattaataa attcgataac
     5641 atccagagct tcctgaaggt gatgcctctc atcggagtca acgctaagtt cgtggaggaa
     5701 tacgcctttt tcaaagactc cgccaagatc gccgatgagc tgaggctgat caagtccttc
     5761 gctagaatgg gagaacctat tgccgatgcc aggagggcca tgtatatcga cgccatccgt
     5821 attttaggaa ccaacctgtc ctatgatgag ctcaaggccc tcgccgacac cttttccctg
     5881 gacgagaacg gaaacaagct caagaaaggc aagcacggca tgagaaattt cattattaat
     5941 aacgtgatca gcaataaaag gttccactac ctgatcagat acggtgatcc tgcccacctc
     6001 catgagatcg ccaaaaacga ggccgtggtg aagttcgtgc tcggcaggat cgctgacatc
     6061 cagaaaaaac agggccagaa cggcaagaac cagatcgaca ggtactacga aacttgtatc
     6121 ggaaaggata agggcaagag cgtgagcgaa aaggtggacg ctctcacaaa gatcatcacc
     6181 ggaatgaact acgaccaatt cgacaagaaa aggagcgtca ttgaggacac cggcagggaa
     6241 aacgccgaga gggagaagtt taaaaagatc atcagcctgt acctcaccgt gatctaccac
     6301 atcctcaaga atattgtcaa tatcaacgcc aggtacgtca tcggattcca ttgcgtcgag
     6361 cgtgatgctc aactgtacaa ggagaaaggc tacgacatca atctcaagaa actggaagag
     6421 aagggattca gctccgtcac caagctctgc gctggcattg atgaaactgc ccccgataag
     6481 agaaaggacg tggaaaagga gatggctgaa agagccaagg agagcattga cagcctcgag
     6541 agcgccaacc ccaagctgta tgccaattac atcaaataca gcgacgagaa gaaagccgag
     6601 gagttcacca ggcagattaa cagggagaag gccaaaaccg ccctgaacgc ctacctgagg
     6661 aacaccaagt ggaatgtgat catcagggag gacctcctga gaattgacaa caagacatgt
     6721 accctgttcg caaacaaggc cgtcgccctg gaagtggcca ggtatgtcca cgcctatatc
     6781 aacgacattg ccgaggtcaa ttcctacttc caactgtacc attacatcat gcagagaatt
     6841 atcatgaatg agaggtacga gaaaagcagc ggaaaggtgt ccgagtactt cgacgctgtg
     6901 aatgacgaga agaagtacaa cgataggctc ctgaaactgc tgtgtgtgcc tttcggctac
     6961 tgtatcccca ggtttaagaa cctgagcatc gaggccctgt tcgataggaa cgaggccgcc
     7021 aagttcgaca aggagaaaaa gaaggtgtcc ggcaattccg gatccggacc taagaaaaag
     7081 aggaaggtgg cggccgctta cccatacgat gttccagatt acgcttaatg tacaaagtgg
     7141 tgaattccgg caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     7201 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7261 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7321 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7381 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7441 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7501 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7561 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     7621 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7681 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7741 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagtaggtc ttgaaaggag
     7801 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     7861 agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact
     7921 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     7981 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     8041 accggttcta gagcgctgcc accatgtcta ggctggacaa gagcaaagtc ataaacggag
     8101 ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
     8161 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
     8221 atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg
     8281 agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac
     8341 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
     8401 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
     8461 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag
     8521 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag
     8581 caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa
     8641 tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg
     8701 acgattttga cttagacatg ctcccagccg atgcccttga cgattttgac cttgacatgc
     8761 tccccggggg atccggcgca acaaacttct ctctgctgaa acaagccgga gatgtcgaag
     8821 agaatcctgg accgatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     8881 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     8941 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     9001 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     9061 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     9121 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     9181 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taaggtacct
     9241 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     9301 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     9361 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     9421 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     9481 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     9541 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     9601 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     9661 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     9721 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     9781 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     9841 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     9901 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     9961 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
    10021 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
    10081 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
    10141 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
    10201 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    10261 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    10321 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    10381 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    10441 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    10501 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
    10561 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
    10621 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    10681 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
    10741 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    10801 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    10861 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
    10921 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
    10981 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
    11041 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
    11101 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
    11161 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
    11221 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
    11281 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
    11341 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
    11401 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
    11461 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
    11521 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    11581 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
    11641 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    11701 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    11761 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    11821 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    11881 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    11941 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    12001 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    12061 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    12121 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    12181 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    12241 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    12301 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    12361 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    12421 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    12481 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    12541 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    12601 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    12661 atcctt
//
