LOCUS       _Aquamarine_LC3_        6519 bp    DNA        circular     15-OCT-2024
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     AssembledSeq
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     rep_origin      1002..1630
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#4682B4"
                     /ApEinfo_revcolor="#4682B4"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     rep_origin      5171..5611
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#4682B4"
                     /ApEinfo_revcolor="#4682B4"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             6144..6519
                     /locus_tag="Kan/neoR"
                     /label="Kan/neoR"
                     /ApEinfo_label="Kan/neoR"
                     /ApEinfo_fwdcolor="#8FBC8F"
                     /ApEinfo_revcolor="#8FBC8F"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             1..416
                     /locus_tag="Kan/neoR(1)"
                     /label="Kan/neoR(1)"
                     /ApEinfo_label="Kan/neoR"
                     /ApEinfo_fwdcolor="#8FBC8F"
                     /ApEinfo_revcolor="#8FBC8F"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     promoter        5910..6106
                     /locus_tag="SV40 early promoter"
                     /label="SV40 early promoter"
                     /ApEinfo_label="SV40 early promoter"
                     /ApEinfo_fwdcolor="#6495ED"
                     /ApEinfo_revcolor="#6495ED"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     Promoter        5678..5706
                     /locus_tag="Amp prom"
                     /label="Amp prom"
                     /ApEinfo_label="Amp prom"
                     /ApEinfo_fwdcolor="#4682B4"
                     /ApEinfo_revcolor="#4682B4"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    5957..6034
                     /locus_tag="SV40 ORI"
                     /label="SV40 ORI"
                     /ApEinfo_label="SV40 ORI"
                     /ApEinfo_fwdcolor="#4682B4"
                     /ApEinfo_revcolor="#4682B4"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3074..3448
                     /locus_tag="LC3B"
                     /label="LC3B"
                     /ApEinfo_label="LC3B"
                     /ApEinfo_fwdcolor="#0000FF"
                     /ApEinfo_revcolor="#0000FF"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2342..3059
                     /locus_tag="Aquamarine"
                     /label="Aquamarine"
                     /ApEinfo_label="Aquamarine"
                     /ApEinfo_fwdcolor="#00FFFF"
                     /ApEinfo_revcolor="#00FFFF"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3455..4873
                     /locus_tag="TdLanYFP"
                     /label="TdLanYFP"
                     /ApEinfo_label="TdLanYFP"
                     /ApEinfo_fwdcolor="#FF8000"
                     /ApEinfo_revcolor="#FF8000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3000..3018
                     /locus_tag="pEGFP_C F"
                     /label="pEGFP_C F"
                     /ApEinfo_label="pEGFP_C F"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    4176..4196
                     /locus_tag="superYFP seq F"
                     /label="superYFP seq F"
                     /ApEinfo_label="superYFP seq F"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    complement(3644..3664)
                     /locus_tag="superYFP seq R"
                     /label="superYFP seq R"
                     /ApEinfo_label="superYFP seq R"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    complement(4955..4974)
                     /locus_tag="pEGFP_Rev"
                     /label="pEGFP_Rev"
                     /ApEinfo_label="pEGFP_Rev"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    complement(2387..2403)
                     /locus_tag="pEGFP_N R"
                     /label="pEGFP_N R"
                     /ApEinfo_label="pEGFP_N R"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2248..2268
                     /locus_tag="pCMV F"
                     /label="pCMV F"
                     /ApEinfo_label="pCMV F"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3227..3229
                     /locus_tag="F52A"
                     /label="F52A"
                     /ApEinfo_label="F52A"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ORIGIN
        1 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
       61 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
      121 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
      181 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
      241 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
      301 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
      361 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
      421 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
      481 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
      541 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
      601 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
      661 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
      721 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
      781 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
      841 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
      901 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
      961 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1141 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     1201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1381 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1441 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1501 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1561 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1621 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1681 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcatgctt agttattaat
     1741 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     1801 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     1861 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     1921 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     1981 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2041 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     2101 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     2161 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     2221 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     2281 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac
     2341 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2401 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2461 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2521 cctcgtgacc accctgagct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa
     2581 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     2641 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2701 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2761 caagctggag tacaactaca tcagcggcaa cgtctatatc accgccgaca agcagaagaa
     2821 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     2881 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2941 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     3001 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc
     3061 cggactcaga tctatgccgt cggagaagac cttcaagcag cgccgcacct tcgaacaaag
     3121 agtagaagat gtccgactta ttcgagagca gcatccaacc aaaatcccgg tgataataga
     3181 acgatacaag ggtgagaagc agcttcctgt tctggataaa acaaagttcc ttgtacctga
     3241 ccatgtcaac atgagtgagc tcatcaagat aattagaagg cgcttacagc tcaatgctaa
     3301 tcaggccttc ttcctgttgg tgaacggaca cagcatggtc agcgtctcca caccaatctc
     3361 agaggtgtat gagagtgaga aagatgaaga tggattcctg tacatggtct acgcctccca
     3421 ggagacgttc gggatgaaat tgtcagtgga attcatggtc tccaaaggag aggaggataa
     3481 catggcctct ctcccagcga cacatgagtt acacatcttt ggctccttca acggtgtgga
     3541 ctttgacatg gtgggtcgtg gcaccggcaa tccaaatgat ggttatgagg agttaaacct
     3601 gaagtccacc aagggtgacc tccagttctc cccctggatt ctggtccctc aaatcgggta
     3661 tggattccat cagtacctgc ccttccccga cgggatgtcg cctttccagg ccgccatgaa
     3721 agatggctcc ggataccaag tccatcgcac aatgcagttt gaagacggtg cctccctgac
     3781 ttccaactac cgctacacct acgagggaag ccacatcaaa ggagagtttc aggtgaaggg
     3841 gactggtttc cctgctgacg gtcctgtgat gaccaactcg ctgaccgctg cggactggtg
     3901 cgtgaccaag atgctgtacc ccaacgacaa aaccatcatc agcacctttg actggactta
     3961 caccactgga aatggcaagc gctaccagag cactgcgcgg accacctaca cctttgccaa
     4021 gccaatggcg gccaacatcc tgaagaacca gccgatgttc gtgttccgta agacggaact
     4081 caagcactcc aagaccgaac tcaacttcaa ggagtggcaa aaggcattta ccgatgtgat
     4141 gtcgactggc actggttcta cgggctcggg ctcctcagga gaggaggata acatggcctc
     4201 tctcccagcg acacatgagt tacacatctt tggctccttc aacggtgtgg actttgacat
     4261 ggtgggtcgt ggcaccggca atccaaatga tggttatgag gagttaaacc tgaagtccac
     4321 caagggtgac ctccagttct ccccctggat tctggtccct caaatcgggt atggcttcca
     4381 tcagtacctg cccttccccg acgggatgtc gcctttccag gccgccatga aagatggctc
     4441 cggataccaa gtccatcgca caatgcagtt tgaagacggt gcctccctga cttccaacta
     4501 ccgctacacc tacgagggaa gccacatcaa aggagagttt caggtgaagg ggactggttt
     4561 ccctgctgac ggtcctgtga tgaccaactc gctgaccgct gcggactggt gcgtgaccaa
     4621 gatgctgtac cccaacgaca aaaccatcat cagcaccttt gactggactt acaccactgg
     4681 aaatggcaag cgctaccaga gcactgcgcg gaccacctac acctttgcca agccaatggc
     4741 ggccaacatc ctgaagaacc agccgatgtt cgtgttccgt aagacggaac tcaagcactc
     4801 caagaccgaa ctcaacttca aggagtggca aaaggcattt accgatgtga tgggcatgga
     4861 cgagctgtac aagtaaagcg gccgcgacgg taccgcgggc ccgggatcca ccggatctag
     4921 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     4981 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     5041 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5101 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt
     5161 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     5221 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     5281 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     5341 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     5401 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     5461 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     5521 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     5581 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     5641 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5701 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
     5761 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     5821 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     5881 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     5941 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6001 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6061 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     6121 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     6181 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6241 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6301 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     6361 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6421 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6481 ccatcatggc tgatgcaatg cggcggctgc atacgcttg  
//
