LOCUS       1_pR-EBFP2-NheI_1x1_-6                 4181 bp    DNA     circular SYN 23-JAN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(64..858)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1861..2577)
                     /note="pLannotate"
                     /label="EBFP2"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1199..1627
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(2835..3138)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(901..1111)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(2631..2834)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(1661..1782)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     rep_origin      complement(3319..3907)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(3966..4013)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(join(4072..4181,1..43))
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc
       61 cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
      121 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
      181 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
      241 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
      301 cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg
      361 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
      421 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
      481 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
      541 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
      601 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
      661 ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag
      721 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
      781 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
      841 tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa
      901 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
      961 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     1021 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     1081 tgagatgcat gctttgcata cttctgcctg ctggggagcc cggggacttt ccacacccta
     1141 actgacacac attccacagc tggttctttc cgcctcagga ctcttccttt aagtactgac
     1201 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     1261 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     1321 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     1381 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     1441 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     1501 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     1561 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     1621 gcgaatttta acaaaatatt aacgcttaca atttacgcct taagatacat tgatgagttt
     1681 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     1741 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     1801 cattttatgt ttcagaattc attatctaga attcgtcttc aattatgaag acatggagcc
     1861 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtgcggaact ccagcaggac
     1921 catgtgatcg cgcttctcgt tggggtcttt gctcagcacg gactgggtgc tcaggtagtg
     1981 gctgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
     2041 gagctgcacg ctgccgtcct ccacgttgtg gcggatcttg aagttcacct tgatgccgtt
     2101 cttctgcttg acggccatga tatagatgtt gtggctgttg aagttgtact ccagcttgtg
     2161 ccccaggatg ttgccgtcct ccttgaagtc gacgcccttc agctcgatgc ggttcactag
     2221 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag gtgccgtcgt ccttgaagaa
     2281 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
     2341 catgtggtcg gggtagcggg cgaagcactg cacgccgtgg ctcagggtgg tcacgagggt
     2401 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgtt
     2461 ggtggcatcg ccctcgccct cgcccctcac gctgaacttg tggccgttta cgtcgccgtc
     2521 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt
     2581 ggcggcggat ccatttcgat aagccagtaa gcacgaggct gatcagcggt agctctgctt
     2641 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
     2701 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
     2761 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
     2821 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     2881 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     2941 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     3001 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     3061 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     3121 accgtaagtt atgtaacggc tagccggaac tccatatatg ggctatgaac taatgacccc
     3181 gtaattgatt actattaata actaatgcat ggcggtaata cggttatcca cagaatcagg
     3241 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3301 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3361 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3421 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3481 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3541 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3601 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3661 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3721 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3781 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3841 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3901 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaccgcg
     3961 tttatgaaca aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc
     4021 gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc
     4081 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac
     4141 gattccgaag cccaaccttt catagaaggc ggcggtggaa t
//
