LOCUS       pR-NheI-mT-Sapphire-4_1x1_                 4181 bp    DNA     circular SYN 05-JAN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(98..892)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1895..2611)
                     /note="pLannotate"
                     /label="mT-Sapphire"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1233..1661
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(2869..3172)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(935..1145)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(2665..2868)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(1695..1816)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     rep_origin      complement(3353..3941)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(4000..4047)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(join(4106..4181,1..77))
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc
       61 gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc
      121 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
      181 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
      241 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
      301 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca
      361 tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
      421 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
      481 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
      541 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
      601 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
      661 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tgcagttcat
      721 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
      781 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
      841 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg
      901 atcctcatcc tgtctcttga tcgatctttg caaaagccta ggcctccaaa aaagcctcct
      961 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     1021 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
     1081 ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg
     1141 cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca cagctggttc
     1201 tttccgcctc aggactcttc ctttaagtac tgacgcgccc tgtagcggcg cattaagcgc
     1261 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     1321 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     1381 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     1441 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     1501 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     1561 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     1621 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     1681 tacaatttac gccttaagat acattgatga gtttggacaa accacaacta gaatgcagtg
     1741 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     1801 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcaga attcattatc
     1861 tagaattcgt cttcaattat gaagacatgg agcccttgta cagctcgtcc atgccgagag
     1921 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
     1981 ctttgctcag cttggactgg atgctcaggt agtggttgtc gggcagcagc acggggccgt
     2041 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgccgccg tcctcgatgt
     2101 tgtggcggat cttgaagttg gccttgatgc cgttcttctg cttgtcggcc atgatataga
     2161 cgttgtggct gttgaagttg tactccagct tgtgccccag gatgttgccg tcctccttga
     2221 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
     2281 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
     2341 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaaca
     2401 ccatcacgcc gtaggagaag gtggtcacga gggtgggcca gggcacgggc agcttgccgg
     2461 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
     2521 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
     2581 cggtgaacag ctcctcgccc ttgctcacca tggtggcggc ggatccattt cgataagcca
     2641 gtaagcacga ggctgatcag cggtagctct gcttatatag acctcccacc gtacacgcct
     2701 accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt
     2761 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
     2821 gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc
     2881 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
     2941 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
     3001 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca
     3061 atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg
     3121 ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa cggctagccg
     3181 gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt aataactaat
     3241 gcatggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3301 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3361 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3421 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3481 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3541 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3601 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3661 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3721 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3781 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3841 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3901 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3961 ctacggggtc tgacgctcag tggaacgaac cgcgtttatg aacaaacgac ccaacaccgt
     4021 gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct
     4081 tccgtgtttc agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc
     4141 gctggaggat catccagccg gcgtcccgga aaacgattcc g
//
