LOCUS       4_pR-mAmetrine-NheI_1x1_-4                 4181 bp    DNA     circular SYN 23-JAN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             join(3933..4181,1..546)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2214..2930
                     /note="pLannotate"
                     /label="mAmetrine"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(3164..3592)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        1653..1956
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        3680..3890
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1957..2160
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    3009..3130
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     rep_origin      884..1472
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    778..825
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             567..719
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
       61 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
      121 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
      181 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
      241 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
      301 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
      361 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
      421 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
      481 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
      541 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
      601 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
      661 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
      721 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
      781 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggttcgt
      841 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
      901 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
      961 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1021 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1081 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1141 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1201 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1261 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1321 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1381 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1441 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1501 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1561 tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta
     1621 gttcatagcc catatatgga gttccggcta gccgttacat aacttacggt aaatggcccg
     1681 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1741 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1801 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1861 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1921 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     1981 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     2041 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     2101 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     2161 accgctgatc agcctcgtgc ttactggctt atcgaaatgg atccgccgcc accatggtga
     2221 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     2281 taaacggcca caagttcagc gtgcgcggcg agggcgaggg cgatgccacc aacggcaagc
     2341 tgaccctgaa gttcatctgc acctccggca agctgcccgt gccctggccc accctcgtga
     2401 ccaccctgtc ttacggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     2461 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc tccttcaagg
     2521 acgacggcag ctacaggacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     2581 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     2641 agtacaacat gaacgtgtgg gacgcgtata tcacggccga caagcagaag aacggcatca
     2701 aagcgaactt caagatcgag cacaacgtcg aggacggcgg cgtgcagctc gccgacgcgt
     2761 accagcagaa cacccccatc ggcgacggct ccgtgctgct gcctgacaac cactacctga
     2821 gcttccagag caagctgttc aaagacccca acgagcagcg cgatcacatg gtcctgctgg
     2881 agttcgttac cgccgccggg atcactcccg gcatggacga gctgtacaag ggctccatgt
     2941 cttcataatt gaagacgaat tctagataat gaattctgaa acataaaatg aatgcaattg
     3001 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3061 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3121 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     3181 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     3241 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     3301 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     3361 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     3421 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     3481 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     3541 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcagtactt
     3601 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     3661 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3721 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3781 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3841 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     3901 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     3961 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     4021 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     4081 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     4141 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt t
//
