LOCUS       pR-NheI-15-2_1x1_                 4175 bp    DNA     circular SYN 01-NOV-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(join(3784..4175,1..403))
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1406..2116)
                     /note="pLannotate"
                     /label="mRuby3"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      744..1172
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(2374..2677)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(446..656)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(2170..2373)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(1206..1327)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     rep_origin      complement(2858..3446)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(3505..3552)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(3611..3763)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
       61 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
      121 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
      181 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac
      241 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
      301 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
      361 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
      421 ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc
      481 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
      541 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
      601 ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
      661 gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct
      721 caggactctt cctttaagta ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      781 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      841 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      901 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
      961 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     1021 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     1081 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     1141 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     1201 cgccttaaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     1261 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     1321 acaagttaac aacaacaatt gcattcattt tatgtttcag aattccgacc atggaattcg
     1381 tcttcaatta tgaagacatg gagcccttgt acagctcgtc catgccacca ccaaggttgc
     1441 tgtatttggc aactgccact tctctttgca ctacgtaggt ttcattgtca ctctcctcga
     1501 tcctttccag gcggtgatca acggcatgga caccgggcat cttgatgttc ccgacggtct
     1561 tttttgacct gtaagttgtc acgaagttgc agtgcagatg gccaccacca tcaactttca
     1621 gtgcgatgtc agtgtatcct ctcagaccac catctgctgg atacatcatc tctgtattag
     1681 gctcccaacc cttggttttc ttctgcatca cgggaccatt ggagggaaag tttacccctc
     1741 tgaccttgac gttgtagacg agctcgccat cctcaaggct ggtgtcctgc gtgacggtga
     1801 cgactccacc atcttcgtat ctcgtaactc tttcccaagt aaaaccctca ggaaaggact
     1861 gtttaaagaa atcagggatg tcggccgggt acttgataaa ggtacggctg ccatacatga
     1921 acgacgtggc aagaatgtca aaggcaaatg gcaggggtcc tccctcgatg actttgatcc
     1981 tcatggtttg cactccctcg tacggtctgc cttctccttc acctgtgcat ttgaattggt
     2041 ggccgttgac cgaaccttcc atgaccacct tcatacgcat attttccttg atcagctctt
     2101 cgcccttaga caccatggtg gcggcggatc catttcgata agccagtaag cacgaggctg
     2161 atcagcggta gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     2221 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     2281 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     2341 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     2401 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     2461 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     2521 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     2581 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     2641 tcagccaggc gggccattta ccgtaagtta tgtaacggct agccggaact ccatatatgg
     2701 gctatgaact aatgaccccg taattgatta ctattaataa ctaatgcatg gcggtaatac
     2761 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2821 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2881 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2941 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3001 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3061 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3121 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3181 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3241 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3301 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3361 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3421 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3481 ctcagtggaa cgaaccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc
     3541 tttttattgc cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta
     3601 gcctccccct agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
     3661 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
     3721 cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc
     3781 cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
     3841 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
     3901 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
     3961 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
     4021 cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg
     4081 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
     4141 agtacgtgct cgctcgatgc gatgtttcgc ttggt
//
