LOCUS       pR-NheI-17-5_1x1_                 4196 bp    DNA     circular SYN 01-NOV-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             1539..2333
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             join(4001..4196,1..536)
                     /note="pLannotate"
                     /label="mCardinal"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(770..1198)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        3440..3743
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        1286..1496
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        3744..3947
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    615..736
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     rep_origin      2671..3259
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    2565..2612
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             2354..2506
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttatgtacgg gagcaagacc ttcatcaacc acacccaggg catccccgat ttctttaagc
       61 agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaggac gggggcgtgc
      121 ttaccgttac ccaggacacc agcctccagg acggctgctt gatctacaac gtcaagctca
      181 gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
      241 ccaccaccga gactctgtac cccgctgacg gcggcctgga aggcagatgc gacatggccc
      301 tgaagctcgt gggcgggggc cacctgcact gcaaccttaa gaccacatac agatccaaga
      361 aacccgctaa gaacctcaag atgcccggcg tctactttgt ggaccgcaga ctggaaagaa
      421 tcaaggaggc cgacaatgag acatacgtcg agcagcacga ggtggctgtg gccagatact
      481 gcgacctccc tagcaaactg gggcacaaac ttaatggcat ggacgagctg tacaagggct
      541 ccatgtcttc ataattgaag acgaattcgt tcacgtgaat tctgaaacat aaaatgaatg
      601 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      661 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      721 tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
      781 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
      841 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
      901 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
      961 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     1021 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     1081 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     1141 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
     1201 gtacttaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     1261 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     1321 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1381 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1441 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1501 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     1561 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     1621 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     1681 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     1741 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     1801 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     1861 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     1921 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     1981 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     2041 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     2101 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     2161 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     2221 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     2281 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     2341 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     2401 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     2461 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     2521 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     2581 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggttcgttcc actgagcgtc
     2641 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2701 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2761 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2821 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2881 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2941 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3001 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3061 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3121 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3181 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3241 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3301 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3361 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
     3421 atatggagtt ccggctagcc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     3481 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     3541 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     3601 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     3661 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     3721 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     3781 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     3841 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     3901 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctacc gctgatcagc
     3961 ctcgtgctta ctggcttatc gaaatggatc cgccgccacc atggtgagca agggcgagga
     4021 gctgatcaag gagaacatgc acatgaagct gtacatggaa ggcaccgtga acaaccacca
     4081 cttcaagtgc accaccgaag gggagggcaa gccctacgag ggcacccaga cccagaggat
     4141 taaggtggtg gagggaggcc ccctgccgtt cgcattcgac atcctggcca cctgct
//
