LOCUS       pM-tPT2A-mT-Sapphire-3_1x1_                 3773 bp    DNA     circular SYN 05-JAN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             258..974
                     /note="pLannotate"
                     /label="mT-Sapphire"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(1167..1595)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        3362..3665
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        join(3666..3773,1..96)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             1724..2380
                     /note="pLannotate"
                     /label="cat"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    1012..1133
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(2424..2545)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        1621..1723
                     /note="pLannotate"
                     /label="cat promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      2599..3187
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             189..241
                     /note="pLannotate"
                     /label="T2A (fragment)"
                     /database="snapgene"
                     /identity="96.2"
                     /match_length="98.1"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
       61 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaagaa gacggctccg
      121 gcgccacaaa cttctctctg ctaaagcaag caggtgatgt tgaagaaaac cccgggcctg
      181 gcagcggcga gggcagggga agtctactta catgcgggga cgtggaggaa aatcccggcc
      241 caggatccgc cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      301 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      361 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      421 ccgtgccctg gcccaccctc gtgaccacct tctcctacgg cgtgatggtg ttcgcccgct
      481 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      541 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      601 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      661 gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaacgtc tatatcatgg
      721 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg
      781 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      841 tgctgcccga caaccactac ctgagcatcc agtccaagct gagcaaagac cccaacgaga
      901 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      961 acgagctgta caagtaagag ttgccattca tgtcttctga aacataaaat gaacttgttt
     1021 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     1081 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta
     1141 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     1201 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     1261 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     1321 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     1381 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     1441 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     1501 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     1561 acccgccgcg cttaatgcgc cgctacaggg cgcgtcagta cttaacggaa gatcacttcg
     1621 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
     1681 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1741 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1801 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1861 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1921 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1981 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     2041 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     2101 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtatc
     2161 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     2221 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     2281 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2341 tgaattacaa cagtactgcg atgagtggca gggcggggcg taatcaaaca tgagaattgg
     2401 tcgcgcgagc aattcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     2461 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     2521 cattataagc tgcaataaac aagttggcat ttcgttccac tgagcgtcag accccgtaga
     2581 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2641 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2701 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2761 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2821 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2881 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2941 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3001 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3061 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3121 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3181 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3241 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     3301 ttagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     3361 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     3421 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     3481 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     3541 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     3601 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     3661 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     3721 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caa
//
