LOCUS       4_pM-mAmetrine-tPT2A_1x1_-8                 3773 bp    DNA     circular SYN 03-FEB-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             2023..2742
                     /note="pLannotate"
                     /label="mAmetrine"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(2932..3360)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        1354..1657
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        1658..1861
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             join(3489..3773,1..372)
                     /note="pLannotate"
                     /label="cat"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    2777..2898
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(416..537)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        3386..3488
                     /note="pLannotate"
                     /label="cat promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      591..1179
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             1954..2006
                     /note="pLannotate"
                     /label="T2A (fragment)"
                     /database="snapgene"
                     /identity="96.2"
                     /match_length="98.1"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
       61 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      121 aaagggttta ttgagaatat gtttttcgta tcagccaatc cctgggtgag tttcaccagt
      181 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      241 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      301 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      361 cagggcgggg cgtaatcaaa catgagaatt ggtcgcgcga gcaattcaga catgataaga
      421 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
      481 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttggc
      541 atttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      601 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      661 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      721 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      781 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      841 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      901 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      961 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1021 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1081 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1141 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1201 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1261 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg
     1321 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1381 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1441 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1501 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1561 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1621 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     1681 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     1741 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     1801 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     1861 tggtttagtg aagaagacgg ctccggcgcc acaaacttct ctctgctaaa gcaagcaggt
     1921 gatgttgaag aaaaccccgg gcctggcagc ggcgagggca ggggaagtct acttacatgc
     1981 ggggacgtgg aggaaaatcc cggcccagga tccgccgcca ccatggtgag caagggcgag
     2041 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     2101 aagttcagcg tgcgcggcga gggcgagggc gatgccacca acggcaagct gaccctgaag
     2161 ttcatctgca cctccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgtct
     2221 tacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     2281 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct ccttcaagga cgacggcagc
     2341 tacaggaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     2401 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacatg
     2461 aacgtgtggg acgcgtatat cacggccgac aagcagaaga acggcatcaa agcgaacttc
     2521 aagatcgagc acaacgtcga ggacggcggc gtgcagctcg ccgacgcgta ccagcagaac
     2581 acccccatcg gcgacggctc cgtgctgctg cctgacaacc actacctgag cttccagagc
     2641 aagctgttca aagaccccaa cgagcagcgc gatcacatgg tcctgctgga gttcgttacc
     2701 gccgccggga tcactcccgg catggacgag ctgtacaagt aagagttgcc attcatgtct
     2761 tctgaaacat aaaatgaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2821 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2881 actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     2941 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     3001 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     3061 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     3121 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     3181 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     3241 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     3301 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     3361 cagtacttaa cggaagatca cttcgtgatc ggcacgtaag aggttccaac tttcaccata
     3421 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
     3481 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
     3541 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
     3601 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     3661 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
     3721 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttc
//
