LOCUS       pM-V-tPT2A-1                 3773 bp    DNA     circular SYN 07-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(59..775)
                     /note="pLannotate"
                     /label="mVenus"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      3211..3639
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(1141..1444)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(937..1140)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(2426..3082)
                     /note="pLannotate"
                     /label="cat"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    2261..2382
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(join(3673..3773,1..21))
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(3083..3185)
                     /note="pLannotate"
                     /label="cat promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(1619..2207)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(792..844)
                     /note="pLannotate"
                     /label="T2A (fragment)"
                     /database="snapgene"
                     /identity="96.2"
                     /match_length="98.1"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ataagctgca ataaacaagt tcattttatg tttcagaaga catgaatggc aactcttact
       61 tgtagagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
      121 tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctggtagctc aggtagtggt
      181 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
      241 gctgcacgcc gccgtcctcg atgttgtggc ggatcttgaa gttggccttg atgccgttct
      301 tctgcttgtc ggcggtgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
      361 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
      421 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
      481 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
      541 tgtggtcggg gtagcgggcg aagcactgca ggccgtagcc cagggtggtc acgagggtgg
      601 gccagggcac gggcagcttg ccggtggtgc agatcagctt cagggtcagc ttgccgtagg
      661 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
      721 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg
      781 cggcggatcc tgggccggga ttttcctcca cgtccccgca tgtaagtaga cttcccctgc
      841 cctcgccgct gccaggcccg gggttttctt caacatcacc tgcttgcttt agcagagaga
      901 agtttgtggc gccggagccg tcttcttcac taaaccagct ctgcttatat agacctccca
      961 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
     1021 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     1081 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
     1141 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     1201 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     1261 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     1321 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     1381 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     1441 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     1501 actaatgcat ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1561 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1621 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1681 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1741 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1801 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1861 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1921 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1981 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2041 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2101 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2161 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2221 tcttttctac ggggtctgac gctcagtgga acgaaatgcc aacttgttta ttgcagctta
     2281 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2341 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gaattgctcg
     2401 cgcgaccaat tctcatgttt gattacgccc cgccctgcca ctcatcgcag tactgttgta
     2461 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     2521 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     2581 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     2641 tggctgatac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     2701 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     2761 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     2821 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat
     2881 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     2941 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     3001 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     3061 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     3121 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     3181 gatcacgaag tgatcttccg ttaagtactg acgcgccctg tagcggcgca ttaagcgcgg
     3241 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3301 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3361 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3421 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3481 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3541 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     3601 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     3661 caatttacgc cttaagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     3721 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc att
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