LOCUS       pM-tPT2A-mPapaya-1_1x1_                 3767 bp    DNA     circular SYN 05-JAN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(join(3474..3767,1..420))
                     /note="pLannotate"
                     /label="mPapaya1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      2856..3284
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(786..1089)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(582..785)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(2071..2727)
                     /note="pLannotate"
                     /label="cat"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    1906..2027
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(3318..3439)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(2728..2830)
                     /note="pLannotate"
                     /label="cat promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(1264..1852)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(437..489)
                     /note="pLannotate"
                     /label="T2A (fragment)"
                     /database="snapgene"
                     /identity="96.2"
                     /match_length="98.1"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gccgtcggcg ggaaaattca cccctctgaa gatacttttg tgatagatgc agttctcctt
       61 ttcagagact gttatgtcca cgttgcatcg acagaccgct ccgtcctcaa agaggtagct
      121 cctttcccac gtatagcccg ctggacatga gttcttgaag taatctacaa tatcctgtgg
      181 gtactctgtg aaaatccggt caccatattt aaaacccgga cttaaaatgt cctccgagaa
      241 cggcagcggg cctccttcta tcacacacaa atttgcggtt tgcttaccct taaaagggtt
      301 tccaatgccc tctccagtaa tgacaaattt gtgaccatta acgcaacctt ccatgtggta
      361 cttcaccgtc atctcctcct ttagtccatg cttcgattgc ccctcgccct tgctcaccat
      421 ggtggcggcg gatcctgggc cgggattttc ctccacgtcc ccgcatgtaa gtagacttcc
      481 cctgccctcg ccgctgccag gcccggggtt ttcttcaaca tcacctgctt gctttagcag
      541 agagaagttt gtggcgccgg agccgtcttc ttcactaaac cagctctgct tatatagacc
      601 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
      661 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
      721 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
      781 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
      841 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
      901 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
      961 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     1021 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     1081 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
     1141 taataactaa tgcatggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1201 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1261 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1321 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1381 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1441 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1501 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1561 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1621 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1681 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1741 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1801 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1861 tttgatcttt tctacggggt ctgacgctca gtggaacgaa atgccaactt gtttattgca
     1921 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1981 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgaatt
     2041 gctcgcgcga ccaattctca tgtttgatta cgccccgccc tgccactcat cgcagtactg
     2101 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     2161 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     2221 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     2281 gggattggct gatacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     2341 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     2401 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     2461 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga attccggatg
     2521 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     2581 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     2641 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     2701 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     2761 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     2821 gtgccgatca cgaagtgatc ttccgttaag tactgacgcg ccctgtagcg gcgcattaag
     2881 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     2941 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3001 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3061 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3121 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3181 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     3241 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     3301 gcttacaatt tacgccttaa gatacattga tgagtttgga caaaccacaa ctagaatgca
     3361 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3421 aagctgcaat aaacaagttc attttatgtt tcagaagaca tgaatggcaa ctcttacttg
     3481 tacagctcgt ccatgcctgc gattgcatgt tctgttaatt gccatttctg gtttttagca
     3541 tcagagcggt cctccctaag gagcttatgc tgtataaaat gccattctgg catcttggag
     3601 ggcactgatt tagctttata taccgtatca aactggcaat gataacgacc gccatccttc
     3661 agcaacaggc acattttgac ctttcccttt aatatccctt gctttggaac aggcacaatt
     3721 ttctcggtac tagcttccca attagtggtc attttcttca ttacggg
//
