LOCUS       14_pM-mOrange2-tPT2A-3                 3764 bp    DNA     circular SYN 27-JAN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             255..962
                     /note="pLannotate"
                     /label="mOrange2"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(1155..1583)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        3350..3653
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        join(3654..3764,1..93)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             1712..2368
                     /note="pLannotate"
                     /label="cat"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    1000..1121
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(2412..2533)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        1609..1711
                     /note="pLannotate"
                     /label="cat promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      2587..3175
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             186..238
                     /note="pLannotate"
                     /label="T2A (fragment)"
                     /database="snapgene"
                     /identity="96.2"
                     /match_length="98.1"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
       61 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaagaagac ggctccggcg
      121 ccacaaactt ctctctgcta aagcaagcag gtgatgttga agaaaacccc gggcctggca
      181 gcggcgaggg caggggaagt ctacttacat gcggggacgt ggaggaaaat cccggcccag
      241 gatccgccgc caccatggtg agcaagggcg aggagaataa catggccatc atcaaggagt
      301 tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      361 gcgagggcga gggccgcccc tacgagggct ttcagaccgc taagctgaag gtgaccaagg
      421 gtggccccct gcccttcgcc tgggacatcc tgtcccctca tttcacctac ggctccaagg
      481 cctacgtgaa gcaccccgcc gacatccccg actacttcaa gctgtccttc cccgagggct
      541 tcaagtggga gcgcgtgatg aactacgagg acggcggcgt ggtgaccgtg acccaggact
      601 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
      661 ccgacggccc cgtgatgcag aagaaaacca tgggctggga ggcctcctcc gagcggatgt
      721 accccgagga cggtgccctg aagggcaaga tcaagatgag gctgaagctg aaggacggcg
      781 gccactacac ctccgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
      841 gcgcctacat cgtcgacatc aagttggaca tcacctccca caacgaggac tacaccatcg
      901 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
      961 agtaagagtt gccattcatg tcttctgaaa cataaaatga acttgtttat tgcagcttat
     1021 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     1081 cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt
     1141 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     1201 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     1261 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     1321 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     1381 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     1441 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     1501 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     1561 taatgcgccg ctacagggcg cgtcagtact taacggaaga tcacttcgtg atcggcacgt
     1621 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
     1681 atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
     1741 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     1801 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     1861 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     1921 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     1981 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     2041 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     2101 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtatcag ccaatccctg
     2161 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     2221 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     2281 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     2341 gtactgcgat gagtggcagg gcggggcgta atcaaacatg agaattggtc gcgcgagcaa
     2401 ttcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     2461 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     2521 caataaacaa gttggcattt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2581 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2641 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2701 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2761 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2821 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2881 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2941 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3001 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3061 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3121 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3181 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3241 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat
     3301 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     3361 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     3421 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     3481 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     3541 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     3601 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     3661 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     3721 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaat
//
