LOCUS       pM-17-tPT2A-10                 3788 bp    DNA     circular SYN 07-DEC-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             2023..2754
                     /note="pLannotate"
                     /label="mCardinal"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(2947..3375)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        1658..1861
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             join(3504..3788,1..372)
                     /note="pLannotate"
                     /label="cat"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    2792..2913
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(416..537)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        3401..3503
                     /note="pLannotate"
                     /label="cat promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      591..1179
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        1294..1657
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="95.8"
                     /fragment="False"
                     /other="enhancer"
     CDS             1954..2006
                     /note="pLannotate"
                     /label="T2A (fragment)"
                     /database="snapgene"
                     /identity="96.2"
                     /match_length="98.1"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
       61 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      121 aaagggttta ttgagaatat gtttttcgta tcagccaatc cctgggtgag tttcaccagt
      181 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      241 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      301 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      361 cagggcgggg cgtaatcaaa catgagaatt ggtcgcgcga gcaattcaga catgataaga
      421 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
      481 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttggc
      541 atttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      601 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      661 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      721 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      781 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      841 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      901 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      961 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1021 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1081 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1141 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1201 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1261 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg
     1321 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1381 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1441 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1501 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1561 cggtaaatgg cctgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1621 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     1681 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     1741 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     1801 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     1861 tggtttagtg aagaagacgg ctccggcgcc acaaacttct ctctgctaaa gcaagcaggt
     1921 gatgttgaag aaaaccccgg gcctggcagc ggcgagggca ggggaagtct acttacatgc
     1981 ggggacgtgg aggaaaatcc cggcccagga tccgccgcca ccatggtgag caagggcgag
     2041 gagctgatca aggagaacat gcacatgaag ctgtacatgg aaggcaccgt gaacaaccac
     2101 cacttcaagt gcaccaccga aggggagggc aagccctacg agggcaccca gacccagagg
     2161 attaaggtgg tggagggagg ccccctgccg ttcgcattcg acatcctggc cacctgcttt
     2221 atgtacggga gcaagacctt catcaaccac acccagggca tccccgattt ctttaagcag
     2281 tccttccctg agggcttcac atgggagaga gtcaccacat acgaggacgg gggcgtgctt
     2341 accgttaccc aggacaccag cctccaggac ggctgcttga tctacaacgt caagctcaga
     2401 ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
     2461 accaccgaga ctctgtaccc cgctgacggc ggcctggaag gcagatgcga catggccctg
     2521 aagctcgtgg gcgggggcca cctgcactgc aaccttaaga ccacatacag atccaagaaa
     2581 cccgctaaga acctcaagat gcccggcgtc tactttgtgg accgcagact ggaaagaatc
     2641 aaggaggccg acaatgagac atacgtcgag cagcacgagg tggctgtggc cagatactgc
     2701 gacctcccta gcaaactggg gcacaaactt aatggcatgg acgagctgta caagtaagtt
     2761 cacgtattca tgtcttctga aacataaaat gaacttgttt attgcagctt ataatggtta
     2821 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     2881 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
     2941 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     3001 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     3061 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     3121 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     3181 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     3241 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     3301 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     3361 cgctacaggg cgcgtcagta cttaacggaa gatcacttcg tgatcggcac gtaagaggtt
     3421 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
     3481 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     3541 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     3601 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     3661 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     3721 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     3781 ccgttttc
//
