LOCUS       Exported                2337 bp ds-DNA     circular SYN 27-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...1d-erzmkv5
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 16, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(842..2337,1..841)
                     /label=source
     source          1091..1222
                     /label=source
     source          1091..1150
                     /label=d
     exon            complement(1..630)
                     /label=Translation 630-1
     primer_bind     393..412
                     /label=Amp-R
     promoter        complement(631..735)
                     /label=AmpR promoter
     primer_bind     796..822
                     /label=PD9385
     misc_feature    798..820
                     /label=gibson left
     promoter        842..1082
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     947..981
                     /label=PG0008
     primer_bind     complement(1057..1090)
                     /label=Primer 1
     primer_bind     complement(1057..1090)
                     /label=Primer 7
     misc_feature    1091..1222
                     /label=best scaffold 211026
     misc_feature    1091..1115
                     /label=regon 1 del
     primer_bind     complement(1186..1222)
                     /label=Primer 2
     misc_feature    1223..1238
                     /label=16bp guide
     primer_bind     1245..1270
                     /label=Primer 4
     misc_feature    1245..1251
                     /label=termination
     misc_feature    1252..1271
                     /label=gibson right
     rep_origin      complement(1348..1936)
                     /direction=LEFT
                     /label=ori
     primer_bind     complement(1352..1367)
                     /label=PG0397
     primer_bind     complement(1428..1447)
                     /label=pBR322ori-F
     CDS             complement(join(2107..2337,1..630))
                     /label=AmpR
     exon            complement(2107..2337)
                     /label=Translation 2337-2107
     primer_bind     complement(2172..2200)
                     /label=Primer 3
     primer_bind     complement(2172..2200)
                     /label=PD9796
ORIGIN
        1 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
       61 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
      121 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
      181 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
      241 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
      301 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
      361 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
      421 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
      481 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
      541 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
      601 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
      661 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
      721 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgcta gctgtacaaa
      781 aaagcaggct ttaaaggaac caattcagtc gactggatcc ggtaccaagg tcgggcagga
      841 agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
      901 gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
      961 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     1021 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     1081 acgaaacacc gctccacttg ctaatgcaag tggtgccttc caaagctata tgctgaggga
     1141 ggatgggcgc tgcggcatgc gtctgcccac ctcagagtgg gtatccttac ctattgaaaa
     1201 gtaataggtc aaggaatgcc gctttccagc aatgtctcag gctgtttttt taagcttggg
     1261 ccgctcgagg tacctctcta catatgacat gtgagcaaaa ggccagcaaa aggccaggaa
     1321 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1381 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1441 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1501 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1561 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1621 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1681 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1741 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1801 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1861 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1921 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1981 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2041 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2101 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2161 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2221 tggccccagt gctgcaatga taccgcgaga tccacgctca ccggctccag atttatcagc
     2281 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgc
//
