LOCUS       pCJ1022                 9644 bp ds-DNA     circular     14-OCT-2024
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            addgene-plasmid-48138-sequence-251928.gbk Cas9 from S. pyogenes
            with 2A-EGFP, and cloning backbone for sgRNA. Added second gRNA
            from Addgene 85451 at XbaI and KpnI sites Maintained in Stbl3 cells
            due to tandem repeat
FEATURES             Location/Qualifiers
     source          1..9644
                     /label="source"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     1..21
                     /label="hU6-F"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        1..241
                     /label="U6 promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     172..191
                     /label="LKO.1 5' BY 4520"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    246..269
                     /label="BY 5743"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    247..269
                     /label="BY 6311"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    250..269
                     /label="MmPkd2 gRNA genomic 24930-24911"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(250..273)
                     /label="BY 5744"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_RNA        270..345
                     /label="tracrRNA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          399..429
                     /label="forward sequencing primer for SapI"
                     /note="sequence: agcgcgtgcgccaattctgcagacaaatggc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(399..429)
                     /label="reverse screening primer for BbsI site"
                     /note="sequence: gccatttgtctgcagaattggcgcacgcgct"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     434..454
                     /label="hU6-F"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        434..674
                     /label="U6 promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    434..789
                     /label="from 85451"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     605..624
                     /label="LKO.1 5' BY 4520"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    680..702
                     /label="BY 5745"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    683..702
                     /label="MmPkd2 gRNA genomic 25053-25034"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(683..704)
                     /label="BY 6314"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(683..705)
                     /label="BY 6312"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(683..705)
                     /label="BY 5746"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_RNA        703..778
                     /label="tracrRNA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(769..795)
                     /label="BY5748"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(795..824)
                     /label="potential primer"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     enhancer        796..1081
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    974..995
                     /label="BY 5697"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        1083..1360
                     /label="chicken beta-actin promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     intron          1361..1588
                     /label="hybrid intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     regulatory      1600..1609
                     /label="KOZAK"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1609..1674
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1681..1701
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1726..5826
                     /label="Cas9"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             5827..5874
                     /label="nucleoplasmin NLS"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             5890..5943
                     /label="T2A"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             5944..6657
                     /label="EGFP"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     complement(5986..6007)
                     /label="EGFP-N"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(6247..6266)
                     /label="EXFP-R"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     6594..6615
                     /label="EGFP-C"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(6685..6702)
                     /label="BGH-rev"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    6691..6898
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     repeat_region   6907..7036
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     repeat_region   6907..7047
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      7122..7577
                     /label="f1 ori"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(7209..7228)
                     /label="F1ori-R"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     7419..7440
                     /label="F1ori-F"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(7594..7613)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     7713..7735
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(7773..7791)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     promoter        7859..7963
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             7964..8824
                     /label="AmpR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(8182..8201)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      8995..9583
                     /label="ori"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     9484..9503
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    9616..9640
                     /label="potential primer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacacca ctcggagttg gcgtagacgg ttttagagct agaaatagca agttaaaata
      301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag
      361 ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag
      421 acaaatggct ctagagggcc tatttcccat gattccttca tatttgcata tacgatacaa
      481 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
      541 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      601 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      661 cttgtggaaa ggacgaaaca ccacgcacgc agttccattc cggttttaga gctagaaata
      721 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
      781 tttttctagg gtacccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      841 cgacccccgc ccattgacgt caatagtaac gccaataggg actttccatt gacgtcaatg
      901 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      961 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattgtg cccagtacat
     1021 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1081 ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc
     1141 aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg
     1201 ggggcgcgcg ccgggggggg gggggggggg gggggggggg gggggggcga ggcggagagg
     1261 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
     1321 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct
     1381 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
     1441 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagctgag
     1501 caagaggtaa gggtttaagg gatggttggt tggtggggta ttaatgttta attacctgga
     1561 gcacctgcct gaaatcactt tttttcaggt tggaccggtg ccaccatgga ctataaggac
     1621 cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc
     1681 ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc
     1741 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     1801 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     1861 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
     1921 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     1981 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     2041 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     2101 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     2161 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     2221 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     2281 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     2341 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     2401 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     2461 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     2521 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     2581 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     2641 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     2701 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     2761 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     2821 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     2881 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     2941 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     3001 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     3061 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     3121 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     3181 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     3241 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     3301 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     3361 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     3421 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     3481 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     3541 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     3601 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     3661 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     3721 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     3781 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     3841 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     3901 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     3961 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     4021 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     4081 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     4141 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     4201 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     4261 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     4321 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     4381 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     4441 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     4501 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     4561 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     4621 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     4681 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     4741 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     4801 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     4861 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     4921 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     4981 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     5041 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     5101 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     5161 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     5221 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     5281 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     5341 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     5401 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     5461 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     5521 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     5581 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     5641 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     5701 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
     5761 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     5821 ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaaggaattc
     5881 ggcagtggag agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc
     5941 ccagtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     6001 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     6061 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     6121 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     6181 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     6241 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     6301 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     6361 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     6421 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     6481 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     6541 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     6601 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggaa
     6661 ttctaactag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6721 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6781 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6841 gggtggggca ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagc
     6901 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     6961 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     7021 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     7081 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     7141 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
     7201 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     7261 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     7321 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     7381 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7441 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     7501 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     7561 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7621 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7681 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7741 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     7801 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     7861 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7921 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7981 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8041 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8101 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8161 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8221 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8281 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8341 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8401 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8461 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8521 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8581 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8641 ctggtttatt gctgataaat ctggagccgg tgagcgtgga agccgcggta tcattgcagc
     8701 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8761 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8821 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8881 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8941 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9001 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9061 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9121 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9181 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9241 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9301 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9361 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9421 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9481 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9541 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9601 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//