LOCUS       plasmid                 5338 bp    DNA     circular SYN 22-OCT-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             3742..4602
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             1551..1724
                     /note="pLannotate"
                     /label="lacZα"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2255..2908
                     /note="pLannotate"
                     /label="GST26_SCHJA"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             346..1428
                     /note="pLannotate"
                     /label="lacI"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        3637..3741
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        268..345
                     /note="pLannotate"
                     /label="lacI promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      join(4773..5338,1..24)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.2"
                     /fragment="False"
                     /other="rep_origin"
     promoter        1477..1507
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        2180..2208
                     /note="pLannotate"
                     /label="tac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    1515..1531
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    2216..2232
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             3069..3320
                     /note="pLannotate"
                     /label="MSI1 (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="23.2"
                     /fragment="True"
                     /other="CDS"
     rep_origin      72..205
                     /note="pLannotate"
                     /label="RSF ori (fragment)"
                     /database="snapgene"
                     /identity="95.5"
                     /match_length="17.9"
                     /fragment="True"
                     /other="rep_origin"
     protein_bind    1441..1462
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="95.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     misc_feature    complement(210..261)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    3492..3532
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="29.1"
                     /fragment="True"
                     /other="misc_feature"
     CDS             3351..3390
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="20.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1988..2104)
                     /note="pLannotate"
                     /label="yciV (fragment)"
                     /database="swissprot"
                     /identity="84.6"
                     /match_length="13.3"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
       61 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      121 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      181 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
      241 tgcggtattt cacaccgcat aaattccgac accatcgaat ggcgcaaaac ctttcgcggt
      301 atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg
      361 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac
      421 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg
      481 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc
      541 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct
      601 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa
      661 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac
      721 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg
      781 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac
      841 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta
      901 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc
      961 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt
     1021 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc
     1081 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt
     1141 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg
     1201 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg
     1261 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa
     1321 agaaaaacca cgctagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1381 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1441 taatgtaagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     1501 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     1561 ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     1621 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     1681 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt
     1741 ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata
     1801 ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg
     1861 tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt
     1921 actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt
     1981 ttgatggcgt tggaattagc ttatcgactg cacggtgcac caatgcttct ggcgtcaggc
     2041 agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc
     2101 tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca
     2161 aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg
     2221 agcggataac aatttcacac aggaaacagt attcatgtcc cctatactag gttattggaa
     2281 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga
     2341 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg
     2401 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat
     2461 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg
     2521 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat
     2581 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat
     2641 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac
     2701 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg
     2761 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat
     2821 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac
     2881 gtttggtggt ggcgaccatc ctccaaaatc ggatctggaa gttctgttcc aggggccccc
     2941 tgggattaat ggacgaaaaa accaccggtt ggcgtggtgg tcacgttgtt gaaggtctgg
     3001 ctggtgaact ggaacagctg cgtgctcgtc tggaacacca cccgcagggt cagcgtgaac
     3061 cgggatcctg caagatgttc atcggaggac tcagttggca gaccacgcag gaagggctgc
     3121 gcgaatactt cggccagttc ggggaggtga aagagtgtct ggtgatgcgg gaccccctga
     3181 ccaaaagatc caggggtttc ggcttcgtca ctttcatgga ccaggcgggg gtggataaag
     3241 tgctggcgca atcgcggcac gagctcgact ccaaaacaat tgaccccaag gtggcctttc
     3301 ctcgaagagc acagcctaag tgagcggccg catcgtgact gactgacgat ctgcctcgcg
     3361 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3421 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     3481 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta taattcttga
     3541 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     3601 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3661 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3721 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3781 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3841 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     3901 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3961 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     4021 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4081 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4141 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4201 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4261 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     4321 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4381 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4441 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4501 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4561 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4621 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4681 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4741 tcagaccccg tagaaaagat caaaggatct tcttgagatc cttttttttc tgcgcgtaat
     4801 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4861 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4921 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4981 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5041 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5101 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5161 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5221 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5281 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcg
//
