LOCUS       plasmid                 5334 bp    DNA     circular SYN 22-OCT-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             418..1278
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             3565..3738
                     /note="pLannotate"
                     /label="lacZα"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             4269..4922
                     /note="pLannotate"
                     /label="GST26_SCHJA"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2360..3442
                     /note="pLannotate"
                     /label="lacI"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        313..417
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        2282..2359
                     /note="pLannotate"
                     /label="lacI promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      1449..2038
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.2"
                     /fragment="False"
                     /other="rep_origin"
     promoter        3491..3521
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        4194..4222
                     /note="pLannotate"
                     /label="tac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    3529..3545
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    4230..4246
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             5079..5330
                     /note="pLannotate"
                     /label="Msi1 (fragment)"
                     /database="swissprot"
                     /identity="98.8"
                     /match_length="23.2"
                     /fragment="True"
                     /other="CDS"
     protein_bind    3455..3476
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="95.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     misc_feature    complement(2224..2275)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    168..208
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="29.1"
                     /fragment="True"
                     /other="misc_feature"
     CDS             27..66
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="20.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4002..4118)
                     /note="pLannotate"
                     /label="yciV (fragment)"
                     /database="swissprot"
                     /identity="84.6"
                     /match_length="13.3"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga cggtgaaaac
       61 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
      121 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
      181 cagtcacgta gcgatagcgg agtgtataat tcttgaagac gaaagggcct cgtgatacgc
      241 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
      301 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      361 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      421 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      481 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      541 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
      601 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      661 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      721 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      781 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
      841 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
      901 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
      961 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1021 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1081 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     1141 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1201 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1261 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     1321 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     1381 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1441 ggatcttctt gagatccttt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1501 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1561 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1621 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1681 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1741 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1801 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1861 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1921 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1981 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2041 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2101 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2161 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2221 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcataaattc
     2281 cgacaccatc gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag
     2341 tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg
     2401 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac
     2461 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca
     2521 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca
     2581 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt
     2641 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct
     2701 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat
     2761 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac
     2821 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct
     2881 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc
     2941 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc
     3001 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa
     3061 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat
     3121 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga
     3181 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg
     3241 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
     3301 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accacgctag cgcccaatac
     3361 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3421 ccgactggaa agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg
     3481 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     3541 aacaatttca cacaggaaac agctatgacc atgattacgg attcactggc cgtcgtttta
     3601 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     3661 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     3721 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa
     3781 agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag
     3841 atgcacggtt acgatgcgcc catctacacc aacgtgacct atcccattac ggtcaatccg
     3901 ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa
     3961 agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttggaat tagcttatcg
     4021 actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg
     4081 tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa
     4141 tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa
     4201 ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     4261 cagtattcat gtcccctata ctaggttatt ggaaaattaa gggccttgtg caacccactc
     4321 gacttctttt ggaatatctt gaagaaaaat atgaagagca tttgtatgag cgcgatgaag
     4381 gtgataaatg gcgaaacaaa aagtttgaat tgggtttgga gtttcccaat cttccttatt
     4441 atattgatgg tgatgttaaa ttaacacagt ctatggccat catacgttat atagctgaca
     4501 agcacaacat gttgggtggt tgtccaaaag agcgtgcaga gatttcaatg cttgaaggag
     4561 cggttttgga tattagatac ggtgtttcga gaattgcata tagtaaagac tttgaaactc
     4621 tcaaagttga ttttcttagc aagctacctg aaatgctgaa aatgttcgaa gatcgtttat
     4681 gtcataaaac atatttaaat ggtgatcatg taacccatcc tgacttcatg ttgtatgacg
     4741 ctcttgatgt tgttttatac atggacccaa tgtgcctgga tgcgttccca aaattagttt
     4801 gttttaaaaa acgtattgaa gctatcccac aaattgataa gtacttgaaa tccagcaagt
     4861 atatagcatg gcctttgcag ggctggcaag ccacgtttgg tggtggcgac catcctccaa
     4921 aatcggatct ggaagttctg ttccaggggc ccctgggatt aatggacgaa aaaaccaccg
     4981 gttggcgtgg tggtcacgtt gttgaaggtc tggctggtga actggaacag ctgcgtgctc
     5041 gtctggaaca ccacccgcag ggtcagcgtg aaccgggatc caagaagatc ttcgtggggg
     5101 ggctgtctgt gaacaccacg gtggaagatg tgaaacacta tttcgagcag ttcggaaagg
     5161 tggatgatgc catgctgatg ttcgacaaaa ccaccaacag gcacagaggg tttggatttg
     5221 tcacgtttga gagcgaggac atcgtagaga aagtttgtga gatccacttc catgaaatca
     5281 acaacaaaat ggtggaatgc aagaaagccc agccaaagga ggtgatgtcc tgag
//
