LOCUS       plasmid                 5319 bp    DNA     circular SYN 22-OCT-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(2994..3854)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(534..707)
                     /note="pLannotate"
                     /label="lacZα"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(join(4669..5319,1..3))
                     /note="pLannotate"
                     /label="GST26_SCHJA"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(830..1912)
                     /note="pLannotate"
                     /label="lacI"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(3855..3959)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(1913..1990)
                     /note="pLannotate"
                     /label="lacI promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(2234..2823)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.2"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(751..781)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(50..78)
                     /note="pLannotate"
                     /label="tac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(727..743)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(26..42)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(4276..4512)
                     /note="pLannotate"
                     /label="MSI2 (fragment)"
                     /database="swissprot"
                     /identity="98.7"
                     /match_length="24.1"
                     /fragment="True"
                     /other="CDS"
     protein_bind    complement(796..817)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="95.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     misc_feature    1997..2048
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(4064..4104)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="29.1"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(4206..4245)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="20.8"
                     /fragment="True"
                     /other="CDS"
     CDS             154..270
                     /note="pLannotate"
                     /label="yciV (fragment)"
                     /database="swissprot"
                     /identity="84.6"
                     /match_length="13.3"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 catgaatact gtttcctgtg tgaaattgtt atccgctcac aattccacac attatacgag
       61 ccgatgatta attgtcaaca gctcatttca gaatatttgc cagaaccgtt atgatgtcgg
      121 cgcaaaaaac attatccaga acgggagtgc gccttgagcg acacgaatta tgcagtgatt
      181 tacgacctgc acagccatac cacagcttcc gatggctgcc tgacgccaga agcattggtg
      241 caccgtgcag tcgataagct aattccaacg ccatcaaaaa taattcgcgt ctggccttcc
      301 tgtagccagc tttcatcaac attaaatgtg agcgagtaac aacccgtcgg attctccgtg
      361 ggaacaaacg gcggattgac cgtaatggga taggtcacgt tggtgtagat gggcgcatcg
      421 taaccgtgca tctgccagtt tgaggggacg acgacagtat cggcctcagg aagatcgcac
      481 tccagccagc tttccggcac cgcttctggt gccggaaacc aggcaaagcg ccattcgcca
      541 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
      601 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      661 tcacgacgtt gtaaaacgac ggccagtgaa tccgtaatca tggtcatagc tgtttcctgt
      721 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      781 agcctggggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc
      841 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      901 aggcggtttg cgtattgggc gctagcgtgg tttttctttt caccagtgag acgggcaaca
      961 gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt
     1021 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt
     1081 cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg
     1141 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa
     1201 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc
     1261 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca
     1321 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac
     1381 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac
     1441 tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag
     1501 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc
     1561 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca
     1621 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt
     1681 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc
     1741 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca
     1801 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct
     1861 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca
     1921 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt
     1981 cgatggtgtc ggaatttatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     2041 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2101 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2161 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2221 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2281 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2341 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2401 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2461 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2521 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2581 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2641 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     2701 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2761 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaaggatct
     2821 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2881 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2941 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3001 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3061 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3121 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3181 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3241 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3301 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3361 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3421 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3481 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3541 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3601 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3661 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3721 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3781 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3841 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3901 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3961 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     4021 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattatacac tccgctatcg
     4081 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     4141 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4201 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagatcgt cagtcagtca
     4261 cgatgcggcc gctcacggct gagctttctt acattctacc attttattat tgatttcatg
     4321 gaaatgaatc tcacagactt tctccacaac atcttcattc tcaaaagtga caaagccaaa
     4381 ccctctgtgc ctgttggtag ttttatcaaa catcagcatt gcatcttcca ccttgccaaa
     4441 ctgctcgaaa tattgcttta catcttccac tactgtgttc gcagataacc cgcctacaaa
     4501 tattttcttg gatcccggtt cacgctgacc ctgcgggtgg tgttccagac gagcacgcag
     4561 ctgttccagt tcaccagcca gaccttcaac aacgtgacca ccacgccaac cggtggtttt
     4621 ttcgtccatt aatcccaggg gcccctggaa cagaacttcc agatccgatt ttggaggatg
     4681 gtcgccacca ccaaacgtgg cttgccagcc ctgcaaaggc catgctatat acttgctgga
     4741 tttcaagtac ttatcaattt gtgggatagc ttcaatacgt tttttaaaac aaactaattt
     4801 tgggaacgca tccaggcaca ttgggtccat gtataaaaca acatcaagag cgtcatacaa
     4861 catgaagtca ggatgggtta catgatcacc atttaaatat gttttatgac ataaacgatc
     4921 ttcgaacatt ttcagcattt caggtagctt gctaagaaaa tcaactttga gagtttcaaa
     4981 gtctttacta tatgcaattc tcgaaacacc gtatctaata tccaaaaccg ctccttcaag
     5041 cattgaaatc tctgcacgct cttttggaca accacccaac atgttgtgct tgtcagctat
     5101 ataacgtatg atggccatag actgtgttaa tttaacatca ccatcaatat aataaggaag
     5161 attgggaaac tccaaaccca attcaaactt tttgtttcgc catttatcac cttcatcgcg
     5221 ctcatacaaa tgctcttcat atttttcttc aagatattcc aaaagaagtc gagtgggttg
     5281 cacaaggccc ttaattttcc aataacctag tatagggga
//
