LOCUS       plasmid                 5979 bp    DNA     circular SYN 22-OCT-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(1933..2793)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(5452..5625)
                     /note="pLannotate"
                     /label="lacZα"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(join(5748..5979,1..851))
                     /note="pLannotate"
                     /label="lacI"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(2794..2898)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(852..929)
                     /note="pLannotate"
                     /label="lacI promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(4240..4891)
                     /note="pLannotate"
                     /label="GST (fragment)"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="99.2"
                     /fragment="True"
                     /other="CDS"
     rep_origin      complement(1173..1762)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.2"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(5669..5699)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(4968..4996)
                     /note="pLannotate"
                     /label="tac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(3215..4249)
                     /note="pLannotate"
                     /label="UNK (fragment)"
                     /database="swissprot"
                     /identity="96.2"
                     /match_length="42.6"
                     /fragment="True"
                     /other="CDS"
     protein_bind    complement(5645..5661)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(4944..4960)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(5714..5735)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="95.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     misc_feature    936..987
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(4892..4909)
                     /note="pLannotate"
                     /label="7xHis (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="85.7"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(3003..3043)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="29.1"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(3145..3184)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="20.8"
                     /fragment="True"
                     /other="CDS"
     CDS             5072..5188
                     /note="pLannotate"
                     /label="yciV (fragment)"
                     /database="swissprot"
                     /identity="84.6"
                     /match_length="13.3"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca
       61 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
      121 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
      181 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
      241 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
      301 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
      361 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
      421 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
      481 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
      541 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
      601 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
      661 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
      721 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
      781 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
      841 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
      901 cgaaaggttt tgcgccattc gatggtgtcg gaatttatgc ggtgtgaaat accgcacaga
      961 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
     1021 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1081 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1141 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1201 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1261 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1321 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1381 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1441 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1501 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1561 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     1621 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1681 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1741 cgcagaaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1801 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1861 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1921 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1981 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2041 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2101 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2161 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2221 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
     2281 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2341 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2401 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2461 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2521 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     2581 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2641 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2701 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2761 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2821 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2881 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     2941 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
     3001 attatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     3061 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     3121 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     3181 gcagatcgtc agtcagtcac gatgcggccg cctagctcac aggcaccgag tctccggcgg
     3241 cagatggcat gtacaggaca ggacccggct gggtggggct ggacacagct gaggaaggct
     3301 gcaggtcgtc actcaggggt ggctgttcta cgtgggcaaa ggcgcagaag ggtcctcggg
     3361 gacagctgcc cgactgctgc atgtcgttgc acttggtgga cttgtagatc tcggggtgga
     3421 actgctgctc ggtgcgggtg tggcagtact ggcaggcgtc tccgttctca cacttgccag
     3481 ggtctcccca ctcatccccg tgcttgacgt ttggacatgg agacgacctg tatttgtgct
     3541 tccgggggct ccgccgccgg tccttgctgt tgtggtagta gggacaggca tagccttggc
     3601 ggcacagccg cgggggcttc ttgcaaggct ccgtcttata gttccccagc acataagcag
     3661 tctcttgcca ccgaggctcc tcgctgagga tcttttctat catggcatgg ctcgcagccc
     3721 cagccgactg gccctctatg ctcccctcta ccgtggtctg gccattctgc aaggcctcca
     3781 tggcctgaag ctccctgatg tcgtagacag gggagcggag gtcatggggc ccgtgggcaa
     3841 aagcgcagtg caggccgttt ttggtgcagt tgcctttcga gtctgtctcg tggatgcaga
     3901 ttccagtttt gtagtaacga aggtggtacc tgcgctcagt gtcccctgtg gttctgtgca
     3961 ggaatgggca ctcgtcgccc tccgggcaga ggcctgtagc ctcgtcgtac ttggtgcagt
     4021 agacgtcagg gctgtaattg aaggtgccgt cccgacggcg gatggaccgg cggcgccgct
     4081 ggttcacgaa gtgccagtgg aagcaggtgt agggccgatg ctgcgtgcat ttgtgttgca
     4141 caaagagtgg gcactgctct gtgcggaatt ctttcaggta cgtgtagtgc tgcggctcga
     4201 gtaatcccag gggcccctgg aacagaactt ccagatccga ttttggagga tggtcgccac
     4261 caccaaacgt ggcttgccag ccctgcaaag gccatgctat atacttgctg gatttcaagt
     4321 acttatcaat ttgtgggata gcttcaatac gttttttaaa acaaactaat tttgggaacg
     4381 catccaggca cattgggtcc atgtataaaa caacatcaag agcgtcatac aacatgaagt
     4441 caggatgggt tacatgatca ccatttaaat atgttttatg acataaacga tcttcgaaca
     4501 ttttcagcat ttcaggtagc ttgctaagaa aatcaacttt gagagtttca aagtctttac
     4561 tatatgcaat tctcgaaaca ccgtatctaa tatccaaaac cgctccttca agcattgaaa
     4621 tctctgcacg ctcttttgga caaccaccca acatgttgtg cttgtcagct atataacgta
     4681 tgatggccat agactgtgtt aatttaacat caccatcaat ataataagga agattgggaa
     4741 actccaaacc caattcaaac tttttgtttc gccatttatc accttcatcg cgctcataca
     4801 aatgctcttc atatttttct tcaagatatt ccaaaagaag tcgagtgggt tgcacaaggc
     4861 ccttaatttt ccaataacct aggatggggg agtgatggtg atggtgatgt ataggggaca
     4921 tgaatactgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacat tatacgagcc
     4981 gatgattaat tgtcaacagc tcatttcaga atatttgcca gaaccgttat gatgtcggcg
     5041 caaaaaacat tatccagaac gggagtgcgc cttgagcgac acgaattatg cagtgattta
     5101 cgacctgcac agccatacca cagcttccga tggctgcctg acgccagaag cattggtgca
     5161 ccgtgcagtc gataagctaa ttccaacgcc atcaaaaata attcgcgtct ggccttcctg
     5221 tagccagctt tcatcaacat taaatgtgag cgagtaacaa cccgtcggat tctccgtggg
     5281 aacaaacggc ggattgaccg taatgggata ggtcacgttg gtgtagatgg gcgcatcgta
     5341 accgtgcatc tgccagtttg aggggacgac gacagtatcg gcctcaggaa gatcgcactc
     5401 cagccagctt tccggcaccg cttctggtgc cggaaaccag gcaaagcgcc attcgccatt
     5461 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     5521 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     5581 acgacgttgt aaaacgacgg ccagtgaatc cgtaatcatg gtcatagctg tttcctgtgt
     5641 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5701 cctggggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt
     5761 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5821 gcggtttgcg tattgggcgc tagcgtggtt tttcttttca ccagtgagac gggcaacagc
     5881 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
     5941 cccagcaggc gaaaatcctg tttgatggtg gttaacggc
//
