LOCUS       YY206:_pAAV_hSyn::GEMIN 7154 bp ds-DNA     circular     23-OCT-2024
DEFINITION  AAV expression of jRGECO1a, a red fluorescent calcium sensor
            protein, from the Synapsin promoter.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            addgene-plasmid-100854-sequence-198193.gbk
FEATURES             Location/Qualifiers
     misc_feature    253..258
                     /label="EcoRI"
     CDS             1228..1263
                     /translation="GGSDYKDDDDK*"
     CDS             1237..1260
                     /label="FLAG"
                     /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site"
                     /translation="DYKDDDDK"
     misc_feature    1264..1269
                     /label="EcoRI"
     promoter        1278..1676
                     /label="UbC promoter"
                     /note="human ubiquitin C promoter"
     misc_feature    3159..3164
                     /label="EcoRI"
     misc_feature    3171..3176
                     /label="HindIII"
     misc_feature    3183..3771
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional regulatory element"
     primer_bind     complement(3236..3256)
                     /label="WPRE-R"
                     /note="WPRE, reverse primer"
     CDS             complement(3654..3665)
                     /label="Factor Xa site"
                     /product="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     primer_bind     complement(3774..3790)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar variants"
     polyA_signal    3820..3941
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3831..3850)
                     /label="EBV-rev"
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3885..3904
                     /label="SV40pA-R"
                     /note="SV40 polyA, reverse primer"
     repeat_region   4103..4232
                     /label="AAV2 ITR"
     primer_bind     complement(4298..4315)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4469..5057)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /direction="LEFT"
     primer_bind     complement(4549..4568)
                     /label="pBR322ori-F"
                     /note="pBR322 origin, forward primer"
     CDS             complement(5228..6088)
                     /label="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"
                     /gene="bla"
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     primer_bind     5851..5870
                     /label="Amp-R"
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6089..6193)
                     /label="AmpR promoter"
                     /gene="bla"
     rep_origin      complement(6219..6674)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
                     /direction="LEFT"
     primer_bind     complement(6356..6377)
                     /label="F1ori-F"
                     /note="F1 origin, forward primer"
     primer_bind     6568..6587
                     /label="F1ori-R"
                     /note="F1 origin, reverse primer"
     repeat_region   6746..6875
                     /label="AAV2 ITR"
     promoter        6957..250
                     /label="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)"
ORIGIN
        1 gcctggcggc gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt cactcgccgg
       61 tcccccgcaa actccccttc ccggccacct tggtcgcgtc cgcgccgccg ccggcccagc
      121 cggaccgcac cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc tgcgctgcgg
      181 cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg tgtcgtgcct
      241 gagagcgcag tcgaattcgc cgccaccATG GTGCGCGGCA TTCGCGGCGC GATTACCGTG
      301 AACAGCGATA CCCCGACTAG CATTATTATT GCGACCATTC TGCTGCTGGA AAAGATGTTG
      361 GAAGCGAACG GCATCCAGAG CTATGAAGAA CTGGCGGCGG TGATTTTTAC CGTTACCGAA
      421 GATCTGACCA GCGCGTTTCC GGCGGAAGCG GCGAGACAGA TTGGCATGCA TCGTGTGCCA
      481 CTGCTGAGCG CGCGTGAAGT GCCAGTTCCA GGTAGCTTGC CGAGAGTGAT TCGCGTGCTG
      541 GCGCTGTGGA ACACCGATAC GCCGCAAGAT CGCGTGAGAC ATGTGTATCT GAGCGAAGCG
      601 GTGCGCCTGC GCCCGGATCT GGAAAGCGCA CAGGGATCCG GGGCTACTAA CTTCAGCCTG
      661 CTGAAGCAGG CTGGAGACGT GGAGGAGAAC CCTGGACCAA GCAAAGCGAA AATTGGCATT
      721 GTGACCGTGA GCGATCGCGC GAGCGCGGGC ATTACCGCAG ATATTAGCGG CAAAGCCATT
      781 ATTCTGGCGC TGAATCTGTA TCTGACCAGC GAATGGGAAC CGATTTATCA GGTGATTCCG
      841 GATGAACAGA AAGTTATTGA ACGCACCCTG ATTAAAATGG CGGATATTCA GGATTGTTGC
      901 CTGATTGTTA CCACCGGCGG CACCGGTCCG GCGAAACGTG ATGTGACTCC TGAAGCAACT
      961 GAAGCGGTGT GCGATCGTAT GATGCCGGGC TTTGGCGAAC TGATGCGCGC GGAAAGCCTG
     1021 AAAGAAGTGC CGACCGCGAT TCTGTCTCGC CAGACTGCGG GTCTGAGAGG TGATAGCCTG
     1081 ATCGTGAACC TGCCGGGTGA TCCGGCCAGC ATTAGCGATT GCCTGCTGGC AGTGTTTCCG
     1141 GCAATTCCGT ATTGCATTGA TCTGATGGAA GGGCCGTATC TGGAATGCAA CGAAGCGATG
     1201 ATCAAACCGT TTCGCCCGAA AGCCAAAGGT GGATCTgact acaaggacga tgatgacaaa
     1261 TGAgaattct taattaaggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct
     1321 cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc
     1381 ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca
     1441 gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt tctttccaga
     1501 gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg
     1561 gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc
     1621 gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggATGa
     1681 tcggtactgg ctttccattc gacccccatt atgtggaagt cctgggcgag cgcatgcact
     1741 acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt cctgcacggt aacccgacct
     1801 cctcctacgt gtggcgcaac atcatcccgc atgttgcacc gacccatcgc tgcattgctc
     1861 cagacctgat cggtatgggc aaatccgaca aaccagacct gggttatttc ttcgacgacc
     1921 acgtccgctt catggatgcc ttcatcgaag ccctgggtct ggaagaggtc gtcctggtca
     1981 ttcacgactg gggctccgct ctgggtttcc actgggccaa gcgcaatcca gagcgcgtca
     2041 aaggtattgc atttatggag ttcatccgcc ctatcccgat ctgggacgaa tggccagaat
     2101 ttgcccgcga gaccttccag gccttccgca ccaccgacgt cggccgcaag ctgatcatcg
     2161 atcagaacgt ttttatcgag ggtacgctgc cgatgggtgt cgtccgcccg ctgactgaag
     2221 tcgagatgga ccattaccgc gagccgttcc tgaatcctgt tgaccgcgag ccactgtggc
     2281 gcttcccaaa cgagctgcca atcgccggtg agccagcgaa catcgtcgcg ctggtcgaag
     2341 aatacatgga ctggctgcac cagtcccctg tcccgaagct gctgttctgg ggcaccccag
     2401 gcgttctgat cccaccggcc gaagccgctc gcctggccaa aagcctgcct aactgcaagg
     2461 ctgtggacat cggcccgggt ctgaatctgc tgcaagaaga caacccggac ctgatcggca
     2521 gcgagatcgc gcgctggctg tcgacgctcg agatttccgg cgagccaacc actGGTGGAT
     2581 CTGGGGGTAG CGGAGGATCA GGGGGTTCCG GAGGTTCTGG CGGAAGTAGC AAAGCGAAAA
     2641 TTGGCATTGT GACCGTGAGC GATCGCGCGA GCGCGGGCAT TACCGCAGAT ATTAGCGGCA
     2701 AAGCCATTAT TCTGGCGCTG AATCTGTATC TGACCAGCGA ATGGGAACCG ATTTATCAGG
     2761 TGATTCCGGA TGAACAGAAA GTTATTGAAC GCACCCTGAT TAAAATGGCG GATATTCAGG
     2821 ATTGTTGCCT GATTGTTACC ACCGGCGGCA CCGGTCCGGC GAAACGTGAT GTGACTCCTG
     2881 AAGCAACTGA AGCGGTGTGC GATCGTATGA TGCCGGGCTT TGGCGAACTG ATGCGCGCGG
     2941 AAAGCCTGAA AGAAGTGCCG ACCGCGATTC TGTCTCGCCA GACTGCGGGT CTGAGAGGTG
     3001 ATAGCCTGAT CGTGAACCTG CCGGGTGATC CGGCCAGCAT TAGCGATTGC CTGCTGGCAG
     3061 TGTTTCCGGC AATTCCGTAT TGCATTGATC TGATGGAAGG GCCGTATCTG GAATGCAACG
     3121 AAGCGATGAT CAAACCGTTT CGCCCGAAAG CCAAATGAga attcgatatc aagcttatcg
     3181 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3241 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3301 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3361 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3421 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3481 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3541 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     3601 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3661 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3721 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     3781 gtcgacctcg acccgggcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
     3841 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     3901 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     3961 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct
     4021 acaaatgtgg taaaatcgat aaggatcttc ctagagcatg gctacgtaga taagtagcat
     4081 ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac tccctctctg
     4141 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     4201 cgggcggcct cagtgagcga gcgagcgcgc agctgcatta atgaatcggc caacgcgcgg
     4261 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     4321 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4381 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4441 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4501 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4561 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4621 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4681 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4741 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4801 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4861 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4921 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4981 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5041 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5101 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5161 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5221 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5281 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5341 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5401 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5461 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5521 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5581 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5641 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5701 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5761 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5821 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5881 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5941 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6001 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6061 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6121 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6181 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     6241 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     6301 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     6361 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     6421 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     6481 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     6541 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     6601 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     6661 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     6721 gcgggcctct tcgctattac gccagctgcg cgctcgctcg ctcactgagg ccgcccgggc
     6781 aaagcccggg cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag
     6841 agagggagtg gccaactcca tcactagggg ttccttgtag ttaatgatta acccgccatg
     6901 ctacttatct acgtagccat gctctaggaa gatctctgca gagggccctg cgtatgagtg
     6961 caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac gaccgacccc
     7021 gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct atcagagagg
     7081 gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca
     7141 gtgccttcgc cccc
//