LOCUS       Trono_Lenti_PEAR       11200 bp DNA     circular SYN 12-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11200)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Oct 22, 2024 from SnapGene 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3379..3384
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1006..1025)
                     /label=SN334
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1171..1535
                     /label=gag
                     /note="gag"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=CPPT; central polypurine tract
                     /note="CPPT; central polypurine tract"
     primer_bind     2480..2509
                     /label=cPPT Fwd
     primer_bind     2608..2657
                     /label=GJ-31
     misc_feature    2608..2637
                     /label=pAF
     misc_feature    2608..2637
                     /label=pAF Gibson
     misc_feature    2634..2637
                     /label=PmeI
     promoter        2638..2878
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        2907..2982
                     /label=gRNA scaffold
     primer_bind     complement(2963..2982)
                     /label=GJ-32
     primer_bind     3069..3100
                     /label=GJ-33
     primer_bind     complement(3086..3136)
                     /label=GJ40
     primer_bind     complement(3106..3136)
                     /label=GJ-32
     primer_bind     3107..3156
                     /label=GJ-33
     misc_feature    3107..3136
                     /label=gAF Gibson
     misc_feature    complement(3107..3136)
                     /label=pAR Gibson
     promoter        3137..3348
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(3142..3163)
                     /label=GJ40
     primer_bind     complement(3329..3378)
                     /label=GJ-34
     primer_bind     complement(3342..3348)
                     /label=FSR-20 (w/o Gibson Adapters)
     primer_bind     3349..3405
                     /label=GJ-35
     primer_bind     3349..3398
                     /label=GJ-41
     misc_feature    3349..3378
                     /label=pBF Gibson
     misc_feature    complement(3349..3378)
                     /label=gAR Gibson
     regulatory      3379..3388
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3385..3669,4467..4901)
                     /codon_start=1
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4882..4931)
                     /label=GJ-36
     primer_bind     complement(4882..4901)
                     /label=GJ-39
     primer_bind     complement(4882..4899)
                     /label=SN343
     primer_bind     4902..4952
                     /label=GJ-37
     misc_feature    4902..4931
                     /label=gBF Gibson
     misc_feature    complement(4902..4931)
                     /label=pBR Gibson
     misc_feature    join(4932..5495,5496..5498,5499..5507)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4944..5496
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     5455..5474
                     /label=IRES_seq1
     primer_bind     5505..5521
                     /label=GJ-41
     CDS             5508..6206
                     /codon_start=1
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     CDS             5508..6203
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     5651..5662
                     /label=GJ-41
     misc_feature    6015..6017
                     /label=Asp170 (insertion site)
     primer_bind     complement(6187..6236)
                     /label=GJ-39
     primer_bind     complement(6187..6206)
                     /label=GJ-36
     primer_bind     complement(6187..6204)
                     /label=SN343
     primer_bind     6207..6255
                     /label=SN335
     misc_feature    6207..6236
                     /label=gBR Gibson
     misc_feature    complement(6207..6236)
                     /label=gBR Gibson
     primer_bind     complement(6217..6266)
                     /label=SN306
     misc_feature    6292..6880
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6763..6774)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6952..7185
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7176..7225)
                     /label=SN334
     polyA_signal    7217..7441
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7487..7915
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7929..8258
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8109..8244
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8306..8353
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8372..8746
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(8583..8596)
                     /label=GJ-36
     primer_bind     complement(8583..8596)
                     /label=SN343
     primer_bind     complement(8583..8596)
                     /label=GJ-39
     polyA_signal    8876..8997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9046..9062)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9070..9086
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9094..9100,9101..9118,9119..9124))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9139..9160
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9448..10036)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(10207..10998,10999..11067))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11068..11172)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgat cagtgtgagg gagtgtaaag ctggtttgag
     2641 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
     2701 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
     2761 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
     2821 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     2881 aacaccgagc tggacggcga cgtaaagttt tagagctaga aatagcaagt taaaataagg
     2941 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcggctacgt gcaggtaagt
     3001 ccgtttacgt cgccgtcctt ttttccgtta cataacttac ggtaaatggc ccaattcgag
     3061 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtcctaa ctcgaacgct
     3121 agctgtgcga tcgtttgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     3181 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     3241 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     3301 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagaa accgctgttc
     3361 ctaggaatcc cgaggcctgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     3421 gtgcccatcc tggtcgaact cgatggagat gtgaacggcc acaagttcag cgtgtccggc
     3481 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3541 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     3601 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3661 tacgtgcaga caagtccgtt tacgtcgccg tccagctcat tttttagtta aaatatggga
     3721 aagtttaaaa gaaggaatga tgaataaact atacaaccac tttaaatatg tgttggtccc
     3781 ctttaagtca gcaagcagat atatcagata tggcagctaa cttaaggagt ccccgtggat
     3841 actggatgaa tagcccccaa ttccaccaat gcaagaacca acagtctatt tggtcttgat
     3901 taggacagta aacttcctgc acaccatagt ctgtgcacta aacaggagta aaacaccctt
     3961 aaggcatctc ttacattggc tgcattaaag tgtgtcactt aatgacatcc gtgatatcag
     4021 ggatagtttg acattggaca tacagttctc tctcagtgat tgtcacctgc ttatatagta
     4081 cgaggaccca tatgtggtca atcactttct tgttgccact ccatgccagg gaagacatag
     4141 ttacaatgag ttataattat aaaagctcag ccttgcatga ggatataaaa tacatttgcc
     4201 atacctctac atatgcctta gttgctttta agctttgttt ggaattgcaa aggccaactt
     4261 cctaaacaca gtttcaaagt attcttttgt cacacctttc cactggatac ttgtattgtc
     4321 tgcaggaaga ggccaccctg cctatgcttc tccttagggc ctcgttctgc agagtcagca
     4381 taaactccca gtctgctcaa ccaattctgt ctgattaggg ccatatctcc ttaccatgag
     4441 ttgatttttg tttctttttt gagcaggagc gcaccatctt cttcaaggac gacggcaact
     4501 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     4561 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     4621 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     4681 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     4741 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     4801 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     4861 ccgccgggat cactctcggc atggacgagc tgtacaagta agacccgaca ttagcgctac
     4921 agcttaagcg gccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     4981 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     5041 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     5101 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     5161 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     5221 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     5281 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     5341 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     5401 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     5461 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg gtgagcaagg
     5521 gcgaggcagt gatcaaggag ttcatgcggt tcaaggtgca catggagggc tccatgaacg
     5581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     5641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttctc ctgggacatc ctgtcccctc
     5701 agttcatgta cggctccagg gccttcacca agcaccccgc cgacatcccc gactactata
     5761 agcagtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     5821 ccgtgaccgt gacccaggac acctccctgg aggacggcac cctgatctac aaggtgaagc
     5881 tccgcggcac caacttccct cctgacggcc ccgtaatgca gaagaagaca atgggctggg
     5941 aagcgtccac cgagcggttg taccccgagg acggcgtgct gaagggcgac attaagatgg
     6001 ccctgcgcct gaaggacggc ggccgctacc tggcggactt caagaccacc tacaaggcca
     6061 agaagcccgt gcagatgccc ggcgcctaca acgtcgaccg caagttggac atcacctccc
     6121 acaacgagga ctacaccgtg gtggaacagt acgaacgctc cgagggccgc cactccaccg
     6181 gcggcatgga cgagctgtac aagtaaaaac gttgttgttt ggggttgaat tactctacat
     6241 gtttaagggt tccggttcca ctaggtacaa ttcgatatca agcttatcga taatcaacct
     6301 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6361 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6421 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6481 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6541 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6601 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6661 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     6721 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6781 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6841 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     6901 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6961 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     7021 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7081 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7141 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cggcgaaacc
     7201 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7261 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7321 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     7381 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     7441 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     7501 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7561 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7621 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7681 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     7741 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7801 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     7861 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     7921 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     7981 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     8041 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     8101 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8161 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     8221 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     8281 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     8341 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     8401 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     8461 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     8521 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     8581 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     8641 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     8701 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     8761 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     8821 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     8881 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8941 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     9001 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     9061 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     9121 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     9181 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     9241 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     9301 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     9361 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     9421 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     9481 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9541 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9601 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9661 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9721 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9781 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9841 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     9901 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9961 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    10021 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    10081 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    10141 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    10201 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    10261 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    10321 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    10381 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    10441 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    10501 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    10561 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    10621 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    10681 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10741 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10801 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10861 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10921 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10981 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    11041 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    11101 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    11161 aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
