LOCUS       trono_gibson_BR        9451 bp DNA     circular SYN 01-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Sam Gould
  TITLE     Direct Submission
  JOURNAL   Exported Oct 22, 2024 from SnapGene 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9451
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1006..1025)
                     /label=SN334
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1171..1535
                     /label=gag
                     /note="gag"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /label=CPPT; central polypurine tract
                     /note="CPPT; central polypurine tract"
     primer_bind     2480..2509
                     /label=cPPT Fwd
     primer_bind     2608..2658
                     /label=Primer 1
     primer_bind     2608..2658
                     /label=Primer 3
     primer_bind     2608..2658
                     /label=Fragment 1.FOR
     primer_bind     2608..2655
                     /label=ZEPCR#17
     misc_feature    2608..2637
                     /label=pAF
     misc_feature    2608..2637
                     /label=pAF
     misc_feature    2608..2637
                     /label=Site #1
     misc_feature    2634..2637
                     /label=PmeI
     promoter        2638..2901
                     /standard_name="hU6"
                     /label=hU6 promoter
     primer_bind     complement(2880..2929)
                     /label=ZEPCR#18
     primer_bind     2885..2895
                     /label=LV5_R
     primer_bind     2903..2929
                     /label=ZEPCR#19
     misc_feature    2903..2908
                     /label=BsmBI
     terminator      2909..2944
                     /standard_name="bidirectional terminator, weaker in 
                     forwarddirection"
     primer_bind     complement(2945..3001)
                     /label=Primer 2
     primer_bind     complement(2945..3001)
                     /label=Fragment 1.REV
     CDS             complement(2959..2965)
                     /standard_name="mRFP"
                     /label=mRFP
     primer_bind     2972..3022
                     /label=Fragment 2.FOR
     misc_feature    complement(3001)
                     /label=gAR
                     /note="gAR"
     misc_feature    3001
                     /label=pBF Gibson
                     /note="pBF Gibson"
     primer_bind     3002..3022
                     /label=FSR-19 (w/o Gibson Adapters)
                     /note="FSR-19 (w/o Gibson Adapters)"
     promoter        3027..3238
                     /note="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     primer_bind     complement(3232..3252)
                     /label=FSR-20 (w/o Gibson Adapters)
                     /note="FSR-20 (w/o Gibson Adapters)"
     misc_feature    3270..3275
                     /label=KOZAK
                     /note="KOZAK"
     CDS             3276..3671
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=Aspergillus terreus BSD
                     /note="BSD"
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     3672..3722
                     /label=FSR-52 (P2A Fwd + 30bp FSR-51 homology)
                     /note="FSR-52 (P2A Fwd + 30bp FSR-51 homology)"
     primer_bind     complement(3672..3701)
                     /label=FSR-51 (Puro Rev + 30bp of P2A Homology)
                     /note="FSR-51 (Puro Rev + 30bp of P2A Homology)"
     CDS             3687..3743
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=2A peptide from porcine teschovirus-1
                     polyprotein
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3744..4457
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=monomeric derivative of red fluorescent protein
                     /note="TagRFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     3744..3764
                     /label=FSR-48 (w/o Gibson Adapters)
                     /note="FSR-48 (w/o Gibson Adapters)"
     primer_bind     complement(4437..4487)
                     /label=FSR-49 (Full Primer)
                     /note="FSR-49 (Full Primer)"
     primer_bind     complement(4437..4487)
                     /label=Fragment 2.REV
     primer_bind     4458..4506
                     /label=SN335
     misc_feature    4458..4487
                     /label=gBR Gibson
     misc_feature    complement(4458..4487)
                     /label=gBR (Rev Comp)
                     /note="gBR (Rev Comp)"
     primer_bind     complement(4468..4517)
                     /label=SN306
     misc_feature    4543..5131
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4942..4961)
                     /label=LV5_R
     CDS             complement(5014..5025)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5203..5436
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5427..5476)
                     /label=SN334
     polyA_signal    5468..5692
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5738..6166
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6180..6509
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        6557..6604
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6623..6997
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7127..7248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7297..7313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7321..7337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7345..7351,7352..7369,7370..7375))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7390..7411
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7699..8287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8458..9249,9250..9318))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9319..9423)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgat cagtgtgagg gagtgtaaag ctggtttaag
     2641 gtcgggcagg aagagggcct atttcccatg attccttcat atttgcatat acgatacaag
     2701 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat
     2761 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     2821 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     2881 ttgtggaaag gacgaaacac ccgagacggt agtcaaaagc ctccggtcgg aggcttttga
     2941 cttggctgag gagtgccatt aagcagaatt cctaactcga acgctagctg tgcgatcgtt
     3001 ttcgagtggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     3061 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     3121 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     3181 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     3241 gtcgtgacgc gggacccgac attagcgctg ccaccatggc caagcctttg tctcaagaag
     3301 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     3361 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     3421 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     3481 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     3541 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     3601 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     3661 tgtgggaggg cgctagcggc agcggcgcca ccaacttcag cctgctgaag caggccggcg
     3721 acgtggagga gaaccccggc cccatggtgt ctaagggcga agagctgatt aaggagaaca
     3781 tgcacatgaa gctgtatatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg
     3841 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     3901 gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc agcagaacct
     3961 tcatcaacca cacccagggc atccccgact tctttaagca gtccttccct gagggcttca
     4021 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     4081 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca
     4141 acggccctgt gatgcagaag aaaacactcg gctgggaggc caacaccgag atgctgtacc
     4201 ccgctgacgg cggcctggaa ggcagaagcg acatggccct gaagctcgtg ggcgggggcc
     4261 acctgatctg caacttcaag accacataca gatccaagaa acccgctaag aacctcaaga
     4321 tgcccggcgt ctactatgtg gaccacagac tggaaagaat caaggaggcc gacaaagaga
     4381 cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg
     4441 ggcacaaact taattgaaaa cgttgttgtt tggggttgaa ttactctaca tgtttaaggg
     4501 ttccggttcc actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac
     4561 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4621 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4681 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4741 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4801 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4861 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4921 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     4981 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5041 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5101 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     5161 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5221 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5281 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5341 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5401 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccggcgaaac ccgctgatca
     5461 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5521 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5581 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5641 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     5701 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     5761 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5821 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5881 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5941 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6001 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6061 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6121 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     6181 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6241 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6301 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     6361 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6421 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     6481 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     6541 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     6601 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     6661 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     6721 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     6781 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     6841 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     6901 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     6961 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     7021 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     7081 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     7141 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7201 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     7261 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7321 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7381 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7441 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7501 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7561 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7621 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7681 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7741 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7801 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7861 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7921 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7981 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8041 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8101 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8161 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8221 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8281 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8341 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8401 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8461 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8521 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8581 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8641 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8701 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8761 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8821 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8881 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8941 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9001 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9061 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9121 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9181 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9241 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9301 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9361 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9421 gcgcacattt ccccgaaaag tgccacctga c
//
