LOCUS       pXPR_BRD051_dCas9-KRAB-MeCP2 13795 bp    DNA     circular SYN 25-OCT-2024
DEFINITION  pXPR_BRD051_dCas9-KRAB-MeCP2.
ACCESSION   pXPR_BRD051_dCas9-KRAB-MeCP2
VERSION     pXPR_BRD051_dCas9-KRAB-MeCP2
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     rep_origin      complement(2122..2550)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(5001..9104)
                     /note="pLannotate"
                     /label="Cas9"
                     /database="snapgene"
                     /identity="99.1"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        complement(12015..12394)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     CDS             complement(1291..1665)
                     /note="pLannotate"
                     /label="BleoR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(9784..10024)
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(10719..10952)
                     /note="pLannotate"
                     /label="RRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     LTR             complement(2851..3084)
                     /note="pLannotate"
                     /label="3' LTR (ΔU3)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     polyA_signal    complement(2596..2820)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(9126..9311)
                     /note="pLannotate"
                     /label="KRAB"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     LTR             complement(11617..11797)
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     misc_feature    complement(11445..11570)
                     /note="pLannotate"
                     /label="HIV-1 Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     polyA_signal    complement(1040..1161)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     misc_feature    complement(10075..10192)
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        12660..12764
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(3773..4796)
                     /note="pLannotate"
                     /label="HygR (fragment)"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="99.8"
                     /fragment="True"
                     /other="CDS"
     CDS             12765..13625
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    complement(3162..3749)
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        complement(1684..1731)
                     /note="pLannotate"
                     /label="EM7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(1779..2108)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(9391..9602)
                     /note="pLannotate"
                     /label="EF-1α core promoter"
                     /database="snapgene"
                     /identity="99.1"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(11815..12014)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="98.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(11098..11461)
                     /note="pLannotate"
                     /label="HIV-1 gag (fragment)"
                     /database="snapgene"
                     /identity="97.3"
                     /match_length="24.2"
                     /fragment="True"
                     /other="CDS"
     misc_RNA        complement(9664..9723)
                     /note="pLannotate"
                     /label="gRNA scaffold (fragment)"
                     /database="snapgene"
                     /identity="98.3"
                     /match_length="78.9"
                     /fragment="True"
                     /other="misc_RNA"
     CDS             complement(10242..10342)
                     /note="pLannotate"
                     /label="Rev (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="CDS"
     protein_bind    947..969
                     /note="pLannotate"
                     /label="lac operator (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="92.0"
                     /fragment="True"
                     /other="protein_bind"
     CDS             complement(1156..1281)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="97.6"
                     /match_length="33.3"
                     /fragment="True"
                     /other="CDS"
     misc_feature    12549..12601
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.6"
                     /fragment="True"
                     /other="misc_feature"
     protein_bind    complement(9347..9364)
                     /note="pLannotate"
                     /label="attB1 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="85.7"
                     /fragment="True"
                     /other="protein_bind"
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3086..3148)
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="90.5"
                     /match_length="21.6"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(2571..2592)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
     CDS             complement(9628..9646)
                     /note="pLannotate"
                     /label="small t antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1321 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1381 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1441 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1501 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     1561 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     1621 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     1681 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     1741 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     1801 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     1861 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1921 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1981 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     2101 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     2581 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     2641 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     2701 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     2761 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     2821 tcgaggctga tcagcgggtt taaacggccc tgctagagat tttccacact gactaaaagg
     2881 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
     2941 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
     3001 aggctcagat ctggtctaac cagagagacc cagtacaagc aaaaagcaga tcttgtcttc
     3061 gttgggagtg aattagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta
     3121 cagctgcctt gtaagtcatt ggtcttaaag tcgacctcga ggcggggagg cggcccaaag
     3181 ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag
     3241 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
     3301 cccgcgcaga atccaggtgg caacacaggc agcagccaag gaaaggacga tgatttcccc
     3361 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
     3421 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
     3481 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
     3541 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     3601 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     3661 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     3721 tcacaaattt tgtaatccag aggttgattg tcgacttaac gcgtgttaac tcattccttt
     3781 gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca
     3841 tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg
     3901 gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca
     3961 accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc
     4021 gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc
     4081 aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc
     4141 tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg
     4201 aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca
     4261 tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac
     4321 acatggggat cagcaatcgc gcatatgaaa tcacgccacg tagtgtattg accgattcct
     4381 tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc
     4441 atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac
     4501 gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctaaa ttccccaatg
     4561 tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgatgaac ataacgatct
     4621 ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg
     4681 aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga cacgctgtcg
     4741 aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatcgat
     4801 ccgcccggtc caggattctc ttcgacatct ccggcttgtt tcagcagaga gaagtttgtt
     4861 gcgccggatc cgctgctgcc gttgctccca ctaccaatgc cgtccacttt ccgctttttc
     4921 ttaggatctt ccaccttgcg ttttttcttg ggggagccgc tgcccaggct ggcataatcg
     4981 ggcacgtcat agggataggc gtcgcctccc agctgagaca ggtcgatccg tgtctcgtac
     5041 aggccggtga tgctctggtg gatcagggtg gcgtccagca cctctttggt gctggtgtac
     5101 ctcttccggt cgatggtggt gtcaaagtac ttgaaggcgg caggggctcc cagattggtc
     5161 agggtaaaca ggtggatgat attctcggcc tgctctctga taggcttgtc tctgtgcttg
     5221 ttgtaggcgc tcagcacctt gtccagatta gcgtcggcca ggatcactct cttggagaac
     5281 tcgctgatct gctcgatgat ctcgtccagg tagtgtttgt gctgttccac aaacagctgt
     5341 ttctgctcat tatcctcggg ggagcccttc agcttctcat agtggctggc caggtacagg
     5401 aagttcacat atttggaggg cagggccagt tcgtttccct tctgcagttc gccggcagag
     5461 gccagcattc tcttccggcc gttttccagc tcgaacaggg agtacttagg cagcttgatg
     5521 atcaggtcct ttttcacttc tttgtagccc ttggcttcca gaaagtcgat gggattcttc
     5581 tcgaagctgc ttctttccat gatggtgatc cccagcagct ctttcacact cttcagtttc
     5641 ttggacttgc ccttttccac tttggccacc accagcacag aataggccac ggtggggctg
     5701 tcgaagccgc cgtacttctt agggtcccag tccttctttc tggcgatcag cttgtcgctg
     5761 ttcctcttgg gcaggataga ctctttgctg aagccgcctg tctgcacctc ggtctttttc
     5821 acgatattca cttggggcat agacagcact ttccgcacgg tggcaaagtc ccggccctta
     5881 tcccacacga tctcgcctgt ttcgccgttt gtctcgatca gaggccgctt ccggatctcg
     5941 ccgttggcca gggtaatctc ggtcttgaaa aagttcatga tgttgctgta gaagaagtac
     6001 ttggcggtag ccttgccgat ttcctgctcg ctcttggcga tcatcttccg cacgtcgtac
     6061 accttgtagt cgccgtacac gaactcgctt tccagcttag ggtacttttt gatcagggcg
     6121 gttcccacga cggcgttcag gtaggcgtcg tgggcgtggt ggtagttgtt gatctcgcgc
     6181 actttgtaaa actggaaatc cttccggaaa tcggacacca gcttggactt cagggtgatc
     6241 actttcactt cccggatcag tttgtcgttc tcgtcgtact tagtgttcat ccgggagtcc
     6301 aggatctgtg ccacgtgctt tgtgatctgc cgggtttcca ccagctgtct cttgatgaag
     6361 ccggccttat ccagttcgct caggccgcct ctctcggcct tggtcagatt gtcgaacttc
     6421 ctctgggtaa tcagcttggc attcagcagc tggcgccagt agttcttcat cttcttcacg
     6481 acctcttcgg agggcacgtt gtcgctcttg ccccggttct tgtcgctccg agtcagcact
     6541 ttgttatcga tggagtcgtc cttcagaaag ctctgaggca cgatagcgtc cacatcgtag
     6601 tcggacagcc ggttgatgtc cagttcctgg tccacgtaca tatcccgccc attctgcagg
     6661 tagtacaggt acagcttctc gttctgcagc tgggtgtttt ccacggggtg ttctttcagg
     6721 atctggctgc ccagctcttt gatgccctct tcgatccgct tcattctctc gcggctgttc
     6781 ttctgtccct tctgggtggt ctggttctct ctggccattt cgatcacgat gttctcgggc
     6841 ttgtgccggc ccatcacttt cacgagctcg tccaccacct tcactgtctg caggatgccc
     6901 ttcttaatgg cggggctgcc ggccagattg gcaatgtgct cgtgcaggct atcgccctgg
     6961 ccggacacct gggctttctg gatgtcctct ttaaaggtca ggctgtcgtc gtggatcagc
     7021 tgcatgaagt ttctgttggc gaagccgtcg gacttcagga aatccaggat tgtcttgccg
     7081 gactgcttgt cccggatgcc gttgatcagc ttccggctca gcctgcccca gccggtgtat
     7141 ctccgccgct tcagctgctt catcactttg tcgtcgaaca ggtgggcata ggttttcagc
     7201 cgttcctcga tcatctctct gtcctcaaac agtgtcaggg tcagcacgat atcttccaga
     7261 atgtcctcgt tttcctcatt gtccaggaag tccttgtcct tgataatttt cagcagatcg
     7321 tggtatgtgc ccagggaggc gttgaaccga tcttccacgc cggagatttc cacggagtcg
     7381 aagcactcga ttttcttgaa gtagtcctct ttcagctgct tcacggtcac tttccggttg
     7441 gtcttgaaca gcaggtccac gatggctttt ttctgctcgc cgctcaggaa ggcgggcttt
     7501 ctcattccct cggtcacgta tttcactttg gtcagctcgt tgtacacggt gaagtactcg
     7561 tacagcaggc tgtgcttggg cagcaccttc tcgttgggca ggttcttatc gaagttggtc
     7621 atccgctcga tgaagctctg ggcgctggcg cccttgtcca ccacttcctc gaagttccag
     7681 ggggtgatgg tttcctcgct ctttctggtc atccaggcga atctgctgtt tcccctggcc
     7741 agagggccca cgtagtaggg gatgcggaag gtcaggatct tctcgatctt ttcccggttg
     7801 tccttcagga atgggtaaaa atcttcctgc cgccgcagaa tggcgtgcag ctctcccagg
     7861 tggatctggt gggggatgct gccgttgtcg aaggtccgct gcttccgcag caggtcctct
     7921 ctgttcagct tcacgagcag ttcctcggtg ccgtccatct tttccaggat gggcttgatg
     7981 aacttgtaga actcttcctg gctggctccg ccatcgatgt agccggcgta gccgttcttg
     8041 ctctggtcga agaaaatctc tttgtacttc tcaggcagct gctgccgcac gagagctttc
     8101 agcagggtca ggtcctggtg gtgctcgtcg tatctcttga tcatagaggc gctcaggggg
     8161 gccttggtga tctcggtgtt cactctcagg atgtcgctca gcaggatggc gtcggacagg
     8221 ttcttggcgg ccagaaacag gtcggcgtac tggtcgccga tctgggccag caggttgtcc
     8281 aggtcgtcgt cgtaggtgtc cttgctcagc tgcagtttgg catcctcggc caggtcgaag
     8341 ttgctcttga agttgggggt caggcccagg ctcagggcaa tcaggttgcc gaacaggcca
     8401 ttcttcttct cgccgggcag ctgggcgatc agattttcca gccgtctgct cttgctcagt
     8461 ctggcagaca ggatggcctt ggcgtccacg ccgctggcgt tgatggggtt ttcctcgaac
     8521 agctggttgt aggtctgcac cagctggatg aacagcttgt ccacgtcgct gttgtcgggg
     8581 ttcaggtcgc cctcgatcag gaagtggccc cggaacttga tcatgtgggc cagggccaga
     8641 tagatcagcc gcaggtcggc cttgtcggtg ctgtccacca gtttctttct caggtggtag
     8701 atggtggggt acttctcgtg gtaggccacc tcgtccacga tgttgccgaa gatggggtgc
     8761 cgctcgtgct tcttatcctc ttccaccagg aaggactctt ccagtctgtg gaagaagctg
     8821 tcgtccacct tggccatctc gttgctgaag atctcttgca gatagcagat ccggttcttc
     8881 cgtctggtgt atcttcttct ggcggttctc ttcagccggg tggcctcggc tgtttctccg
     8941 ctgtcgaaca gcagggcgcc gatcaggttc ttcttgatgc tgtgccggtc ggtgttgccc
     9001 agcaccttga atttcttgct gggcaccttg tactcgtcgg tgatcacggc ccagcccaca
     9061 gagttggtgc cgatggccag gccgatgctg tacttcttgt ccatcgaccc tccgccggaa
     9121 ccgccgggct cttctccctt ctccaaccgg aggatcacat ctggcttagt aagctgataa
     9181 cccaaggaaa ccaggttctt atagttctcc agcatcacat ttctgtacac gatctgctga
     9241 gcagtgtcca gcagcttcca ctcctccctg gtgaagtcca caaatacatc cttgaaggtc
     9301 accagtgtcc gggaccaggc agttagtgac ttagcatcca tgccaacttt tttgtacaaa
     9361 cttgttgatc aatcgatgct agaaccggtc ctgtgttctg gcggcaaacc cgttgcgaaa
     9421 aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct cgggaaaaag
     9481 gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc
     9541 ggatcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg tgcgctctgc
     9601 ccactgacgg gcaccggagc caattcccac tcctttcaag acctagctag cgaattcaaa
     9661 aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta tttcaacttg
     9721 ctacagcatt tctgctgtag ctctgaaaca gagacgcaaa aaaccggtcg tctccggtgt
     9781 ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa
     9841 gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt
     9901 actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattctaa
     9961 ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc
    10021 cctcggtacc ttaattaacc aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt
    10081 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
    10141 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat
    10201 gaatactgcc atttgtctgc agaattggcg cacgcagtgc cgatccgttc actaatcgaa
    10261 tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc ctgtcgggtc
    10321 ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat
    10381 tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta
    10441 tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc
    10501 atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa
    10561 ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg
    10621 tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag
    10681 tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt
    10741 atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca
    10801 acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag
    10861 acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg
    10921 ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca
    10981 ccactcttct ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat
    11041 atttatataa ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca
    11101 gcggccgctt gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa
    11161 gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta
    11221 ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt
    11281 atttgtctac agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc
    11341 tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc tggccttaac
    11401 cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca
    11461 tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc
    11521 tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt
    11581 ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact
    11641 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
    11701 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
    11761 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaggcaaaa cgcgctgctt
    11821 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
    11881 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
    11941 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
    12001 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
    12061 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
    12121 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
    12181 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
    12241 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
    12301 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
    12361 gattactatt aataactagt caataatcaa tgtcaacgcg tatatctggc ccgtacatcg
    12421 cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct
    12481 tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca
    12541 gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    12601 aggggatcgg gagatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc
    12661 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    12721 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    12781 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    12841 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    12901 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    12961 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    13021 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    13081 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    13141 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    13201 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    13261 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    13321 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    13381 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    13441 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    13501 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    13561 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    13621 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    13681 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    13741 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
