LOCUS       Exported               19914 bp ds-DNA    circular SYN 28-10ÔÂ-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    ePE6d
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19914)
  AUTHORS   XRF
  TITLE     Direct Submission
  JOURNAL   Exported 2024Äê10ÔÂ28ÈÕ from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19914
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..26
                     /label="RB"
                     /note="RB"
     misc_feature    274..708
                     /note="35S promoter"
     misc_feature    709..1266
                     /note="CmYLCV-U6"
     misc_feature    1274..1345
                     /note="tGly"
     misc_feature    1452..2558
                     /label="SpR"
                     /note="SpR"
     misc_feature    2566..2607
                     /note="Q1"
     misc_feature    2608..2675
                     /note="HDV"
     misc_feature    2676..2954
                     /note="polyT-HSPt"
     promoter        2961..4939
                     /label="ZmUBI promoter"
                     /note="ZmUBI promoter"
     misc_feature    4947..4952
                     /note="KOZ"
     CDS             4959..4979
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /note="
                     "
                     /protein_id="
                     "
                     /translation="PKKKRKV"
     CDS             4980..9080
                     /codon_start=1
                     /label="OsCas9"
                     /note="Cas9 H840A"
                     /note="rice codon optimized spCas9"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    5637..5639
                     /note="R221K"
     misc_feature    6156..6157
                     /note="N394K"
     misc_feature    7932..7934
                     /note="Feature 18"
     CDS             8719..8737
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /note="nCas9"
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10 mutation in the RuvC-like catalytic domain"
                     /protein_id="
                     "
                     /translation="PEDNE"
     misc_feature    9081..9128
                     /note="XTEN"
     misc_feature    9129..9296
                     /note="optimized NC"
     CDS             9354..9374
                     /note="SV40 NLS"
     misc_feature    9399..10940
                     /note="M-MLV RT-?RNase H"
     CDS             10944..10964
                     /codon_start=1
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             10974..11000
                     /codon_start=1
                     /note="c-myc NLS"
                     /translation="PAAKRVKLD"
     terminator      11010..11274
                     /label="35s terminator"
                     /note="35s terminator"
     CDS             12339..13364
                     /codon_start=1
                     /label="Hyg"
                     /note="Hyg"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     misc_feature    13655..13680
                     /label="LB"
                     /note="LB"
     CDS             14105..14899
                     /codon_start=1
                     /label="KmR"
                     /note="KmR"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      15190..15470
                     /direction=RIGHT
                     /label="pBR322 origin"
                     /note="pBR322 origin"
     misc_feature    complement(16244..16438)
                     /label="pVS1 ori"
                     /note="pVS1 ori"
     misc_feature    16444..16449
                     /label="Muatated BsaI"
                     /note="Muatated BsaI"
     CDS             complement(16504..17577)
                     /codon_start=1
                     /label="RepA"
                     /note="RepA"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(18006..18635)
                     /codon_start=1
                     /label="StaA"
                     /note="StaA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgccaag cttatggagt caaagattca aatagaggac
      301 ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat
      361 gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
      421 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
      481 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
      541 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
      601 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      661 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgattg gcagacatac
      721 tgtcccacaa atgaagatgg aatctgtaaa agaaaacgcg tgaaataatg cgtctgacaa
      781 aggttaggtc ggctgccttt aatcaatacc aaagtggtcc ctaccacgat ggaaaaactg
      841 tgcagtcggt ttggcttttt ctgacgaaca aataagattc gtggccgaca ggtgggggtc
      901 caccatgtga aggcatcttc agactccaat aatggagcaa tgacgtaagg gcttacgaaa
      961 taagtaaggg tagtttggga aatgtccact cacccgtcag tctataaata cttagcccct
     1021 ccctcattgt taagggagca aaatctcaga gagatagtcc tagagagaga aagagagcaa
     1081 gtagcctaga agtagtcaag gcggcgaagt attcaggcac gtggccagga agaagaaaag
     1141 ccaagacgac gaaaacaggt aagagctaag catctagaaa gttgaaaaca atcttcaaaa
     1201 gtcccacatc gcttagataa gaaaacgaag ctgagtttat atacagctag agtcgaagta
     1261 gtgattgaac aaagcaccag tggtctagtg gtagaatagt accctgccac ggtacagacc
     1321 cgggttcgat tcccggctgg tgcaagagac caacccagtg gacataagcc tgttcggttc
     1381 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1441 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     1501 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     1561 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     1621 tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     1681 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     1741 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     1801 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     1861 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     1921 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     1981 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2041 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2101 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2161 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2221 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2281 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2341 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     2401 tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg
     2461 cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca
     2521 agtctgcttt tattattttt aagcgtgcat aataagccgg tctcattgac gcggttctat
     2581 ctagttacgc gttaaaccaa ctagaaaggc cggcatggtc ccagcctcct cgctggcgcc
     2641 ggctgggcaa catgcttcgg catggcgaat gggacttttt tttgatatct ccggggctaa
     2701 ttgaatatga agatgaagat gaaatatttg gtgtgtcaaa taaaaagctg gtgtgcttaa
     2761 gtttgtgttt ttttcttggc ttgttgtgtt atgaatttgt ggctttttct aatattaaat
     2821 gaatgtaaga tctcattata atgaataaac aaatgtttct ataatccatt gtgaatgttt
     2881 tgttggatct cttctgcagc atataactac tgtatgtgct atggtatgga ctatggaata
     2941 tgattaaaga taagaagctt tgcagcgtga cccggtcgtg cccctctcta gagataatga
     3001 gcattgcatg tctaagttat aaaaaattac cacatatttt ttttgtcaca cttgtttgaa
     3061 gtgcagttta tctatcttta tacatatatt taaactttac tctacgaata atataatcta
     3121 tagtactaca ataatatcag tgttttagag aatcatataa atgaacagtt agacatggtc
     3181 taaaggacaa ttgagtattt tgacaacagg actctacagt tttatctttt tagtgtgcat
     3241 gtgttctcct ttttttttgc aaatagcttc acctatataa tacttcatcc attttattag
     3301 tacatccatt tagggtttag ggttaatggt ttttatagac taattttttt agtacatcta
     3361 ttttattcta ttttagcctc taaattaaga aaactaaaac tctattttag tttttttatt
     3421 taataattta gatataaaat agaataaaat aaagtgacta aaaattaaac aaataccctt
     3481 taagaaatta aaaaaactaa ggaaacattt ttcttgtttc gagtagataa tgccagcctg
     3541 ttaaacgccg tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg
     3601 ccaagcgaag cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc
     3661 cgctccaccg ttggacttgc tccgctgtcg gcatccagaa atgcgtggcg gagcggcaga
     3721 cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcacggcagc tacgggggat
     3781 tcctttccca ccgctccttc gctttccctt cctcgcccgc cgtaataaat agacaccccc
     3841 tccacaccct ctttccccaa cctcgtgttg ttcggagcgc acacacacac aaccagatct
     3901 cccccaaatc cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc tccccccccc
     3961 cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac
     4021 ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta
     4081 cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt
     4141 ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt
     4201 gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt
     4261 gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt
     4321 gggcggtcgt tctagatcgg agtagaattc tgtttcaaac tacctggtgg atttattaat
     4381 tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg
     4441 aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag
     4501 atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc
     4561 tagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta
     4621 tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg
     4681 ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat
     4741 ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa
     4801 ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt
     4861 tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc
     4921 ctgttgtttg gtgttacttc tgcagggcca ccatggcccc aaagaagaag cgcaaggtcg
     4981 acaagaagta ctccatcggc ctcgacatcg gcaccaattc tgttggctgg gccgtgatca
     5041 ccgacgagta caaggtgccg tccaagaagt tcaaggtcct cggcaacacc gaccgccact
     5101 ccatcaagaa gaatctcatc ggcgccctgc tgttcgactc tggcgagaca gccgaggcta
     5161 caaggctcaa gaggaccgct agacgcaggt acaccaggcg caagaaccgc atctgctacc
     5221 tccaagagat cttctccaac gagatggcca aggtggacga cagcttcttc cacaggctcg
     5281 aggagagctt cctcgtcgag gaggacaaga agcacgagcg ccatccgatc ttcggcaaca
     5341 tcgtggatga ggtggcctac cacgagaagt acccgaccat ctaccacctc cgcaagaagc
     5401 tcgtcgactc caccgataag gccgacctca ggctcatcta cctcgccctc gcccacatga
     5461 tcaagttcag gggccacttc ctcatcgagg gcgacctcaa cccggacaac tccgatgtgg
     5521 acaagctgtt catccagctc gtgcagacct acaaccagct gttcgaggag aacccgatca
     5581 acgcctctgg cgttgacgcc aaggctattc tctctgccag gctctctaag tcccgcaagc
     5641 tcgagaatct gatcgcccaa cttccgggcg agaagaagaa tggcctcttc ggcaacctga
     5701 tcgccctctc tcttggcctc accccgaact tcaagtccaa cttcgacctc gccgaggacg
     5761 ccaagctcca gctttccaag gacacctacg acgacgacct cgacaatctc ctcgcccaga
     5821 ttggcgatca gtacgccgat ctgttcctcg ccgccaagaa tctctccgac gccatcctcc
     5881 tcagcgacat cctcagggtg aacaccgaga tcaccaaggc cccactctcc gcctccatga
     5941 tcaagaggta cgacgagcac caccaggacc tcacactcct caaggccctc gtgagacagc
     6001 agctcccaga gaagtacaag gagatcttct tcgaccagtc caagaacggc tacgccggct
     6061 acatcgatgg cggcgcttct caagaggagt tctacaagtt catcaagccg atcctcgaga
     6121 agatggacgg caccgaggag ctgctcgtga agctcaagag agaggacctc ctccgcaagc
     6181 agcgcacctt cgataatggc tccatcccgc accagatcca cctcggcgag cttcatgcta
     6241 tcctccgcag gcaagaggac ttctacccgt tcctcaagga caaccgcgag aagattgaga
     6301 agatcctcac cttccgcatc ccgtactacg tgggcccgct cgccaggggc aactccaggt
     6361 tcgcctggat gaccagaaag tccgaggaga caatcacccc ctggaacttc gaggaggtgg
     6421 tggataaggg cgcctctgcc cagtctttca tcgagcgcat gaccaacttc gacaagaacc
     6481 tcccgaacga gaaggtgctc ccgaagcact cactcctcta cgagtacttc accgtgtaca
     6541 acgagctgac caaggtgaag tacgtgaccg aggggatgag gaagccagct ttccttagcg
     6601 gcgagcaaaa gaaggccatc gtcgacctgc tgttcaagac caaccgcaag gtgaccgtga
     6661 agcagctcaa ggaggactac ttcaagaaaa tcgagtgctt cgactccgtc gagatctccg
     6721 gcgtcgagga taggttcaat gcctccctcg ggacctacca cgacctcctc aagattatca
     6781 aggacaagga cttcctcgac aacgaggaga acgaggacat cctcgaggac atcgtgctca
     6841 ccctcaccct cttcgaggac cgcgagatga tcgaggagcg cctcaagaca tacgcccacc
     6901 tcttcgacga caaggtgatg aagcagctga agcgcaggcg ctataccggc tggggcaggc
     6961 tctctaggaa gctcatcaac ggcatccgcg acaagcagtc cggcaagacg atcctcgact
     7021 tcctcaagtc cgacggcttc gccaaccgca acttcatgca gctcatccac gacgactccc
     7081 tcaccttcaa ggaggacatc caaaaggccc aggtgtccgg ccaaggcgat tccctccatg
     7141 aacatatcgc caatctcgcc ggctccccgg ctatcaagaa gggcattctc cagaccgtga
     7201 aggtggtgga cgagctggtg aaggtgatgg gcaggcacaa gccagagaac atcgtgatcg
     7261 agatggcccg cgagaaccag accacacaga agggccaaaa gaactcccgc gagcgcatga
     7321 agaggatcga ggagggcatt aaggagctgg gctcccagat cctcaaggag cacccagtcg
     7381 agaacaccca gctccagaac gagaagctct acctctacta cctccagaac ggccgcgaca
     7441 tgtacgtgga ccaagagctg gacatcaacc gcctctccga ctacgacgtg gacgctattg
     7501 tgccgcagtc cttcctgaag gacgactcca tcgacaacaa ggtgctcacc cgctccgaca
     7561 agaacagggg caagtccgat aacgtgccgt ccgaagaggt cgtcaagaag atgaagaact
     7621 actggcgcca gctcctcaac gccaagctca tcacccagag gaagttcgac aacctcacca
     7681 aggccgagag aggcggcctt tccgagcttg ataaggccgg cttcatcaag cgccagctcg
     7741 tcgagacacg ccagatcaca aagcacgtgg cccagatcct cgactcccgc atgaacacca
     7801 agtacgacga gaacgacaag ctcatccgcg aggtgaaggt catcaccctc aagtccaagc
     7861 tcgtgtccga cttccgcaag gacttccagt tctacaaggt gcgcgagatc aacaactacc
     7921 accacgccca cgacgcctac ctcaatgccg tggtgggcac agccctcatc aagaagtacc
     7981 caaagctcga gtccgagttc gtgtacggcg actacaaggt gtacgacgtg cgcaagatga
     8041 tcgccaagtc cgagcaagag atcggcaagg cgaccgccaa gtacttcttc tactccaaca
     8101 tcatgaattt cttcaagacc gagatcacgc tcgccaacgg cgagattagg aagaggccgc
     8161 tcatcgagac aaacggcgag acaggcgaga tcgtgtggga caagggcagg gatttcgcca
     8221 cagtgcgcaa ggtgctctcc atgccgcaag tgaacatcgt gaagaagacc gaggttcaga
     8281 ccggcggctt ctccaaggag tccatcctcc caaagcgcaa ctccgacaag ctgatcgccc
     8341 gcaagaagga ctgggacccg aagaagtatg gcggcttcga ttctccgacc gtggcctact
     8401 ctgtgctcgt ggttgccaag gtcgagaagg gcaagagcaa gaagctcaag tccgtcaagg
     8461 agctgctggg catcacgatc atggagcgca gcagcttcga gaagaaccca atcgacttcc
     8521 tcgaggccaa gggctacaag gaggtgaaga aggacctcat catcaagctc ccgaagtaca
     8581 gcctcttcga gcttgagaac ggccgcaaga gaatgctcgc ctctgctggc gagcttcaga
     8641 agggcaacga gcttgctctc ccgtccaagt acgtgaactt cctctacctc gcctcccact
     8701 acgagaagct caagggctcc ccagaggaca acgagcaaaa gcagctgttc gtcgagcagc
     8761 acaagcacta cctcgacgag atcatcgagc agatctccga gttctccaag cgcgtgatcc
     8821 tcgccgatgc caacctcgat aaggtgctca gcgcctacaa caagcaccgc gataagccaa
     8881 ttcgcgagca ggccgagaac atcatccacc tcttcaccct caccaacctc ggcgctccag
     8941 ccgccttcaa gtacttcgac accaccatcg accgcaagcg ctacacctct accaaggagg
     9001 ttctcgacgc caccctcatc caccagtcta tcacaggcct ctacgagaca cgcatcgacc
     9061 tctcacaact cggcggcgat agtggttctg aaactcctgg aacatcagaa tcggcgacgc
     9121 cggagagcgc taccgtcgtg tccgggcaga aacaagatag acaaggcggc gagaggaggc
     9181 gtagccagct ggaccgcgac cagtgcgcct actgcaagga gaagggccac tgggcaaaag
     9241 attgtccaaa gaagccccgc gggccaagag gacctcggcc gcagacctcc ctcctttcag
     9301 gcggctccag cggcggctct aagcggaccg ccgacggatc agagttcgag agcccgaaga
     9361 agaagaggaa ggtgtccggc ggctcatctg gcggctccac actcaatatc gaggacgagt
     9421 acaggctgca tgagacatcc aaggagcctg acgtctccct cggcagcaca tggctctcag
     9481 atttcccaca ggcctgggcc gagacaggcg gcatgggcct cgccgtccgc caggcgccgc
     9541 tcatcattcc actgaaggcg acctccacac cggtgagcat caagcagtac ccaatgtctc
     9601 aggaggcaag gctgggcatc aagccacaca ttcagaggct cctggaccag ggcattctgg
     9661 tgccttgcca gagcccgtgg aacacccctc tcctgccggt gaagaagcct ggcacaaatg
     9721 actaccgccc ggtccaggat ctcagggagg tgaacaagcg cgtcgaggat atccatccga
     9781 acgtcccgaa cccatacaat ctcctgtcag gcctcccgcc atctcaccag tggtacaccg
     9841 tgctcgacct gaaggatgcg ttcttctgcc tcaggctgca tccaacaagc cagcctctct
     9901 tcgccttcga gtggcgcgat ccggagatgg gcatttcagg ccagctcacc tggacacggc
     9961 tgccacaggg cttcaagaac tctcctaccc tcttctgtga ggcgctccat cgggacctgg
    10021 ccgatttcag gatccagcac ccagacctca ttctcctcca gtattatgac gatctcctgc
    10081 tcgccgcgac atccgagctg gattgccagc agggaacccg cgcgctgctc cagacactgg
    10141 gaaatctggg atacagggca tcagcgaaga aggcacagat ctgccagaag caggtcaagt
    10201 acctcggcta cctgctcaag gagggacaga ggtggctgac agaggcaagg aaggagacag
    10261 tgatgggcca gcctaccccg aagacaccac ggcagctcag ggagttcctg ggcaaggcgg
    10321 gcttctgccg cctcttcatc ccaggattcg cggagatggc ggcgccactc taccctctga
    10381 ccaagcctgg cacactgttc aactggggac cagaccagca gaaggcgtac caggagatta
    10441 agcaggccct gctcacagca cctgccctcg gcctgccgga cctcacaaag ccattcgagc
    10501 tgttcgtgga tgagaagcag ggctacgcga agggagtcct gacacagaag ctgggaccat
    10561 ggaggcgccc agtggcctac ctctccaaga agctggaccc agtggctgcc ggctggcctc
    10621 cgtgcctgag gatggtggcg gccattgccg tcctcaccaa ggatgccggc aagctgacaa
    10681 tgggccagcc tctcgtcatt ctggcgccgc atgcggtgga ggcgctcgtc aagcagccac
    10741 ctgataggtg gctgtccaac gcgcgcatga cccactacca ggccctgctc ctggacacag
    10801 atagggtgca gttcggccca gtggtcgccc tcaatcctgc cacactgctg ccactccctg
    10861 aggagggcct ccagcataac tgcctcgata ttctggcgga ggcccatgga acccgccctg
    10921 acctcacaga tcagccgctg agcccgaaga agaagaggaa agtgggatca ggaccagccg
    10981 ccaagagggt gaagctcgat taggagctcc ggccgggagc atgcgacgtc gatctaactg
    11041 actagccgcg gccatgctag agtccgcaaa aatcaccagt ctctctctac aaatctatct
    11101 ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct
    11161 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
    11221 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg acctgaattc
    11281 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    11341 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    11401 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    11461 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag cagcttgcca
    11521 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
    11581 accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
    11641 attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca
    11701 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc
    11761 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
    11821 cttcaaagca agtggattga tgtgataaca tggtggagca cgacactctc gtctactcca
    11881 agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg
    11941 taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga
    12001 cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg
    12061 ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
    12121 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
    12181 ctgacgtaag ggatgacgca caatcccact atccttcgca agaccttcct ctatataagg
    12241 aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc
    12301 tctctcgagc tttcgcagat cccggggggc aatgagatat gaaaaagcct gaactcaccg
    12361 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
    12421 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
    12481 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
    12541 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggagtttagc gagagcctga
    12601 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
    12661 tgcccgctgt tctacaaccg gtcgcggagg ctatggatgc gatcgctgcg gccgatctta
    12721 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
    12781 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
    12841 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
    12901 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
    12961 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
    13021 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
    13081 acttcgagcg gaggcatccg gagcttgcag gatcgccacg actccgggcg tatatgctcc
    13141 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
    13201 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
    13261 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
    13321 gtggaaaccg acgccccagc actcgtccga gggcaaagaa atagagtaga tgccgaccgg
    13381 atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag
    13441 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
    13501 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
    13561 agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc
    13621 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
    13681 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
    13741 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
    13801 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
    13861 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
    13921 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
    13981 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
    14041 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
    14101 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
    14161 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
    14221 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
    14281 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
    14341 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
    14401 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
    14461 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
    14521 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
    14581 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
    14641 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
    14701 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
    14761 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
    14821 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
    14881 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
    14941 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    15001 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    15061 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    15121 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    15181 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    15241 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    15301 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    15361 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    15421 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    15481 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    15541 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    15601 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    15661 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    15721 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
    15781 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
    15841 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
    15901 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
    15961 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
    16021 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
    16081 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
    16141 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
    16201 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
    16261 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
    16321 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
    16381 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
    16441 aaacagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
    16501 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
    16561 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
    16621 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
    16681 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
    16741 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
    16801 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
    16861 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
    16921 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
    16981 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
    17041 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
    17101 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
    17161 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
    17221 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
    17281 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
    17341 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
    17401 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
    17461 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
    17521 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc
    17581 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt
    17641 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
    17701 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
    17761 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
    17821 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
    17881 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
    17941 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
    18001 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
    18061 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
    18121 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
    18181 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
    18241 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
    18301 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
    18361 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
    18421 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
    18481 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
    18541 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
    18601 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
    18661 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
    18721 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
    18781 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
    18841 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
    18901 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
    18961 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
    19021 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
    19081 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
    19141 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
    19201 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
    19261 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
    19321 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
    19381 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
    19441 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
    19501 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
    19561 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
    19621 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
    19681 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
    19741 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
    19801 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
    19861 aaatggacga acggataaac cttttcacgc ccttttaaat atccgttatt ctaa
//
