LOCUS       K4-PB-TO_TDP43dNLS-V5- 15307 bp ds-DNA     circular     01-JUL-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     primer_bind     complement(128..147)
                     /label="E60"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(265..1122)
                     /label="Amp"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(651..671)
                     /label="E59"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     967..986
                     /label="F4"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     complement(1112..1139)
                     /label="HH28"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     1934..1953
                     /label="BII(R)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     repeat_region   1996..2008
                     /label="5'TR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     repeat_region   2009..2039
                     /label="5'ITR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     repeat_region   2040..2041
                     /label="5'TR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     repeat_region   2043..2058
                     /label="5'TR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     enhancer        2234..2465
                     /label="core insul"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     2479..2505
                     /label="CC60"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     source          2487..3156
                     /label="color: #ffffff"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_signal     2487..3156
                     /label="SeqVer (WT AAV R)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     source          2487..3156
                     /label="color: #ffffff"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     source          2487..3156
                     /label="color: #ffffff"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_signal     2487..3156
                     /label="SeqVer (SV40 F)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_signal     2487..3156
                     /label="SeqVer (WT AAV R)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2487..3156
                     /label="short UCOE"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2487..3156
                     /label="Synthesized DNA from GeneWiz"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_signal     2487..3156
                     /label="SeqVer (SV40 F)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     2498..2517
                     /label="A43-151119"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(2732..2751)
                     /label="A44-151119"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     2861..2880
                     /label="C54"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     2962..2981
                     /label="EE38"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     complement(3131..3150)
                     /label="A45-151119"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        3163..3540
                     /label="TRE3G promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3163..3540
                     /label="added via PCR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     complement(3232..3254)
                     /label="B28-160212-F"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     3431..3450
                     /label="A34-151006"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     3450..3470
                     /label="A13-MM-Tre3GF"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     complement(3476..3495)
                     /label="A42-151006"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     3516..3535
                     /label="C37"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          3529..3583
                     /label="P031"
                     /note="sequence: taccctcgtaaaggatccgccgccaccatgTCTGAATATATTCGGGTAACCGAAG"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     3531..3558
                     /label="KK09"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     3531..3558
                     /label="BB28-160401"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3547..3555
                     /label="Kozac"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     3547..3558
                     /label="151028_XF_hNEUROG2_3F"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_signal     3547..5592
                     /label="SeqVer (SV40 F)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_signal     3547..5592
                     /label="SeqVer (WT AAV R)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_signal     3547..5592
                     /label="SeqVer (TRE 3G F)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3547..5592
                     /label="synthesized"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             3556..3558
                     /label="start"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3559..4797
                     /label="hTDP-Stop"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             3559..5589
                     /label="Translation 3559-5589"
     primer          3715..3734
                     /label="10083"
                     /note="sequence: GTCCGGCTGGTAGAAGGAAT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     variation       3799..3801
                     /label="K82A"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     variation       3802..3804
                     /label="R83A"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     variation       3805..3807
                     /label="K84A"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     variation       3838..3840
                     /label="K95A"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3838..3849
                     /label="dNLS2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     variation       3844..3846
                     /label="K97A"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     variation       3847..3849
                     /label="R98A"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer          complement(4060..4081)
                     /label="dNLS REV"
                     /note="sequence: TGCAGTCACACCATCGTCCATC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          4143..4162
                     /label="10084"
                     /note="sequence: GCGCTGTACAGAGGACATGA"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          4491..4510
                     /label="10085"
                     /note="sequence: CTTTGGTGCGTTCAGCATTA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     variation       4498..4500
                     /label="A315T"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     variation       4564..4566
                     /label="M337V"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     variation       4597..4599
                     /label="G348C"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer          complement(4775..4815)
                     /label="10101"
                     /note="sequence: GTTGGGGATGGGCTTGCCCATTCCCCAGCCAGAAGACTTAG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             4798..4839
                     /label="V5 tag"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4798..4839
                     /label="On primer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          4798..4862
                     /label="10104"
                     /note="sequence: GGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGACAGCACCGGAAAGTCTTACCCAACTGTGAG"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             4840..5586
                     /label="APEX2"
                     /ApEinfo_revcolor=#3366ff
                     /ApEinfo_fwdcolor=#3366ff
     primer          5030..5049
                     /label="10002"
                     /note="sequence: AACTGGCTCACAGCGCTAAC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          5236..5255
                     /label="P003"
                     /note="sequence: CCCACTAAGGGTTCTGACCA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          5409..5428
                     /label="10003"
                     /note="sequence: CACGGAGTTGTTGAGTGGTG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(5568..5619)
                     /label="P043"
                     /note="sequence: gtgggcgatcgattcgcgagtttaaacttaTCAGGCATCAGCAAACCCAAGC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    5587..5589
                     /label="On primer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(5590..5618)
                     /label="KK10"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(5590..5619)
                     /label="BB29-160401"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_signal     5607..5784
                     /label="SeqVer (SV40 F)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_signal     5607..5975
                     /label="SeqVer (WT AAV R)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_signal     5607..6160
                     /label="SeqVer (TRE 3G F)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(5611..5628)
                     /label="C38"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     5614..5633
                     /label="C12"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     polyA_signal    5623..5753
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(5732..5751)
                     /label="C31"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     5821..5840
                     /label="B19-160210"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(5821..5840)
                     /label="A18-MM-SV40PA_1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5824..5843)
                     /label="P037"
                     /note="sequence: ttgtgtggaattgtgagcgg"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     source          join(5983..15307,1..5982)
                     /label="source"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     complement(6079..6112)
                     /label="r-PciI-RGBpolyA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     polyA_signal    complement(6097..6551)
                     /label="RBGpolyA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     6369..6391
                     /label="B11-151217-R"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     6419..6438
                     /label="A1-151002-R"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(6527..6537)
                     /label="152028_CG_hISL1_5R"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(6561..7308)
                     /label="rtTA3G"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(6702..6714)
                     /label="A37-151006"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(6796..6807)
                     /label="B77"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     6932..6945
                     /label="A35-151006"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     6937..6947
                     /label="151028_CG_hLHX3_IT.F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(7208..7224)
                     /label="A40-151006"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(7269..7308)
                     /label="DD39"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     7287..7306
                     /label="B54-160309"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     intron          7315..7408
                     /label="Mini-Intron"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(7333..7351)
                     /label="A2-151002-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(7385..7405)
                     /label="AA27-151016-1F"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(7390..7410)
                     /label="A41-151006"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     enhancer        complement(7416..8663)
                     /label="CAG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(7633..7652)
                     /label="A26-151102-F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(7640..7660)
                     /label="B04-151217-F"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(8398..8417)
                     /label="B03-151217-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     complement(8624..8643)
                     /label="B16-160126-F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     enhancer        8665..9044
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     8812..8830
                     /label="B10-151217-R"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    9045..9198
                     /label="Transcription blocker"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     9062..9083
                     /label="f-17-trans.block."
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    9199..10409
                     /label="chick cHS4 insulator(1)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     source          10410..10443
                     /label="color: #ffffff"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    10410..10443
                     /label="loxP"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        10444..10655
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     intron          10656..11594
                     /label="EF-1-alpha intron A"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(10691..10710)
                     /label="E22"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     10734..10753
                     /label="E56"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     10890..10907
                     /label="D74"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(10893..10912)
                     /label="E1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     11291..11310
                     /label="D75"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     11369..11388
                     /label="D48"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(11543..11562)
                     /label="E20"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     11551..11571
                     /label="EF1a-F"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    11611..11616
                     /label="Kozak"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             11617..12213
                     /label="PuroR"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     11762..11781
                     /label="B40-160210"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(11933..11951)
                     /label="B41-160210"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     12113..12132
                     /label="B31B-160210"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     12114..12133
                     /label="B18-160210"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     12114..12133
                     /label="B42-160210"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     12220..12239
                     /label="E10"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     complement(12223..12239)
                     /label="B63-T2A-rev"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             12223..12276
                     /label="T2A"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    12283..12309
                     /label="mycNLS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             12283..12309
                     /label="Translation 12283-12309"
     misc_feature    12325..12351
                     /label="mycNLS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             12325..12351
                     /label="Translation 12325-12351"
     CDS             12379..13089
                     /label="mTagBFP2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     12779..12797
                     /label="A75-151217-F"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     12779..12797
                     /label="B05-151217-F"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    13093..13108
                     /label="PCR2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     polyA_signal    13109..13333
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     13119..13138
                     /label="E55"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(13235..13255)
                     /label="C06-160304-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    13334..13367
                     /label="loxP"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     source          13334..13367
                     /label="color: #ffffff"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     enhancer        complement(13379..13609)
                     /label="core insul(1)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     repeat_region   13883..13917
                     /label="3'ITR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     14436..14455
                     /label="B19-160210"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(14436..14455)
                     /label="pLac(F)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(14436..14455)
                     /label="A18-MM-SV40PA_1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(14439..14458)
                     /label="P037"
                     /note="sequence: ttgtgtggaattgtgagcgg"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(14871..14891)
                     /label="E61"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
       61 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      121 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      181 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      241 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
      301 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      361 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      421 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
      481 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
      541 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
      601 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
      661 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
      721 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
      781 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
      841 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
      901 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
      961 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1021 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1081 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1141 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1201 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     1261 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     1321 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     1381 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     1441 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     1501 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     1561 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     1621 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     1681 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     1741 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     1801 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     1861 gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg gcagactcgc
     1921 ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac gctgcagaac
     1981 acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa agataatcat
     2041 gtgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt attaatttga
     2101 atagatatta agttttatta tatttacact tacatactaa taataaattc aacaaacaat
     2161 ttatttatgt ttatttattt attaaaaaaa acaaaaactc aaaatttctt ctataaagta
     2221 acaaaacttt tatgagggac agcccccccc caaagccccc agggatgtaa ttacgtccct
     2281 cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc
     2341 gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc
     2401 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg
     2461 gaaaaggcct ccacggcctt aattaaggga ggtggtccct gcagttacgc caatgataac
     2521 ccccgccaga aaaatcttag tagccttccc tttttgtttt ccgtgcccca actcggcgga
     2581 ttgactcggc cccttccgga aacacccgaa tcaacttcta gtcaaattat tgttcacgcc
     2641 gcaatgaccc acccctggcc cgcgtctgtg gaactgaccc ctggtgtaca ggagagttcg
     2701 ctgctgaaag tggtcccaaa ggggtactag tttttaagct cccaactccc cctcccccag
     2761 cgtctggagg attccacacc ctcgcaccgc aggggcgagg aagtgggcgg agtccggttt
     2821 tggcgccagc cgctgaggct gccaagcaga aaagccaccg ctgaggagac tccggtcact
     2881 gtcctcgccc cgcctccccc ttccctcccc ttggggacca ccgggcgcca cgccgcgaac
     2941 ggtaagtgcc gcggtcgtcg gcgcctccgc cctcccccta gggccccaat tcccagcggg
     3001 cgcggcgcgc ggcccctccc cccgccgggc gcgcgcccgc tgccccgccc ttcgtggccg
     3061 cccggcgtgg gcggtgccac ccctcccccc ggcggccccg cgcgcagctc ccggctccct
     3121 cccccttcgg atgtggcttg agctgtaggc gcggagggta ccagtttact ccctatcagt
     3181 gatagagaac gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta
     3241 ctccctatca gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt
     3301 atgaccagtt tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt
     3361 gatagagaac gtatatccag tttactccct atcagtgata gagaacgtat aagctttagg
     3421 cgtgtacggt gggcgcctat aaaagcagag ctcgtttagt gaaccgtcag atcgcctgga
     3481 gcaattccac aacacttttg tcttatacca actttccgta ccacttccta ccctcgtaaa
     3541 ggatccgccg ccaccatgTC TGAATATATT CGGGTAACCG AAGATGAGAA CGATGAGCCC
     3601 ATTGAAATAC CATCGGAAGA CGATGGGACG GTGCTGCTCT CCACGGTTAC AGCCCAGTTT
     3661 CCAGGGGCGT GTGGGCTTCG CTACAGGAAT CCAGTGTCTC AGTGTATGAG AGGTGTCCGG
     3721 CTGGTAGAAG GAATTCTGCA TGCCCCAGAT GCTGGCTGGG GAAATCTGGT GTATGTTGTC
     3781 AACTATCCAA AAGATAACGC AGCAGCAATG GATGAGACAG ATGCTTCATC AGCAGTGAAA
     3841 GTGAAAAGAG CAGTCCAGAA AACATCCGAT TTAATAGTGT TGGGTCTCCC ATGGAAAACA
     3901 ACCGAACAGG ACCTGAAAGA GTATTTTAGT ACCTTTGGAG AAGTTCTTAT GGTGCAGGTC
     3961 AAGAAAGATC TTAAGACTGG TCATTCAAAG GGGTTTGGCT TTGTTCGTTT TACGGAATAT
     4021 GAAACACAAG TGAAAGTAAT GTCACAGCGA CATATGATAG ATGGACGATG GTGTGACTGC
     4081 AAACTTCCTA ATTCTAAGCA AAGCCAAGAT GAGCCTTTGA GAAGCAGAAA AGTGTTTGTG
     4141 GGGCGCTGTA CAGAGGACAT GACTGAGGAT GAGCTGCGGG AGTTCTTCTC TCAGTACGGG
     4201 GATGTGATGG ATGTCTTCAT CCCCAAGCCA TTCAGGGCCT TTGCCTTTGT TACATTTGCA
     4261 GATGATCAGA TTGCGCAGTC TCTTTGTGGA GAGGACTTGA TCATTAAAGG AATCAGCGTT
     4321 CATATATCCA ATGCCGAACC TAAGCACAAT AGCAATAGAC AGTTAGAAAG AAGTGGAAGA
     4381 TTTGGTGGTA ATCCAGGTGG CTTTGGGAAT CAGGGTGGAT TTGGTAATAG CAGAGGGGGT
     4441 GGAGCTGGTT TGGGAAACAA TCAAGGTAGT AATATGGGTG GTGGGATGAA CTTTGGTGCG
     4501 TTCAGCATTA ATCCAGCCAT GATGGCTGCC GCCCAGGCAG CACTACAGAG CAGTTGGGGT
     4561 ATGATGGGCA TGTTAGCCAG CCAGCAGAAC CAGTCAGGCC CATCGGGTAA TAACCAAAAC
     4621 CAAGGCAACA TGCAGAGGGA GCCAAACCAG GCCTTCGGTT CTGGAAATAA CTCTTATAGT
     4681 GGCTCTAATT CTGGTGCAGC AATTGGTTGG GGATCAGCAT CCAATGCAGG GTCGGGCAGT
     4741 GGTTTTAATG GAGGCTTTGG CTCAAGCATG GATTCTAAGT CTTCTGGCTG GGGAATGGGC
     4801 AAGCCCATCC CCAACCCCCT GCTGGGCCTG GACAGCACCG GAAAGTCTTA CCCAACTGTG
     4861 AGTGCTGATT ACCAGGACGC CGTTGAGAAG GCGAAGAAGA AGCTCAGAGG CTTCATCGCT
     4921 GAGAAGAGAT GCGCTCCTCT AATGCTCCGT TTGGCATTCC ACTCTGCTGG AACCTTTGAC
     4981 AAGGGCACGA AGACCGGTGG ACCCTTCGGA ACCATCAAGC ACCCTGCCGA ACTGGCTCAC
     5041 AGCGCTAACA ACGGTCTTGA CATCGCTGTT AGGCTTTTGG AGCCACTCAA GGCGGAGTTC
     5101 CCTATTTTGA GCTACGCCGA TTTCTACCAG TTGGCTGGCG TTGTTGCCGT TGAGGTCACG
     5161 GGTGGACCTA AGGTTCCATT CCACCCTGGA AGAGAGGACA AGCCTGAGCC ACCACCAGAG
     5221 GGTCGCTTGC CCGATCCCAC TAAGGGTTCT GACCATTTGA GAGATGTGTT TGGCAAAGCT
     5281 ATGGGGCTTA CTGACCAAGA TATCGTTGCT CTATCTGGGG GTCACACTAT TGGAGCTGCA
     5341 CACAAGGAGC GTTCTGGATT TGAGGGTCCC TGGACCTCTA ATCCTCTTAT TTTCGACAAC
     5401 TCATACTTCA CGGAGTTGTT GAGTGGTGAG AAGGAAGGTC TCCTTCAGCT ACCTTCTGAC
     5461 AAGGCTCTTT TGTCTGACCC TGTATTCCGC CCTCTCGTTG ACAAATATGC AGCGGACGAA
     5521 GATGCCTTCT TTGCTGATTA CGCTGAGGCT CACCAAAAGC TTTCCGAGCT TGGGTTTGCT
     5581 GATGCCTGAt aagtttaaac tcgcgaatcg atcgcccacc ccaacttgtt tattgcagct
     5641 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5701 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     5761 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5821 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5881 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5941 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6001 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6061 cggcgagcgg tatcagctca ctcaaaggac ggccgcacat gtaaggaaaa ttttagggat
     6121 gttaaagaaa aaaataacac aaaacaaaat ataaaaaaaa tctaacctca agtcaaggct
     6181 tttctatgga ataaggaatg gacagcaggg ggctgtttca tatactgatg acctctttat
     6241 agccaacctt tgttcatggc agccagcata tgggcatatg ttgccaaact ctaaaccaaa
     6301 tactcattct gatgttttaa atgatttgcc ctcccatatg tccttccgag tgagagacac
     6361 aaaaaattcc aacacactat tgcaatgaaa ataaatttcc tttattagcc agaagtcaga
     6421 tgctcaaggg gcttcatgat gtccccataa tttttggcag agggaaaaag atctcagtgg
     6481 tatttgtgag ccagggcatt ggccacacca gccaccacct tctgataggc agcctgcacc
     6541 tgaggagtga attcacgcgt tttagcctgg cagcatatcg agatcgaagt catcgagagc
     6601 gtcagcaggg agcatatcca gatcaaaatc atccagggca tcggcgggca gcatgtccag
     6661 gtcgaagtcg tccagggcgt cggtgggtcc gccgctctcg cacttcagct gcttctcgag
     6721 gccgcagatg atcagttcca ggccgaacag gaaggcgggc tcggcgccct gtctgtcgaa
     6781 cagctcgatg gcctgcttca gcaggggggg catgctgtcg gtggtgggtg tctctctttc
     6841 ctctttggcg acctggtgtt cctgttcttc cagcacgcag cccagggtga agtggcccac
     6901 ggcgctcagg gcgtacaggg cgttttccag gctgaagccc tgctggcaca ggaaggccag
     6961 ctggttttcc agtgtctcgt actgcttctc ggtgggtctg gtgcccaggt gcactttggc
     7021 gccgtcccgg tggctcagca gggcgcatct gtagctcttg gcgttgttcc gcaggaagtc
     7081 ctgccagctc tcgccttcca gagggcagct gtgggtgtgg tgccggtcca gcatctcgat
     7141 gggcagggcg tccagcaggg cccgcttgtt cttcacgtgc cagtacaggg tgggctgttc
     7201 cacgcccagt ttctgggcca gcttccgggt ggtcaggccc tcgatgccca cgccgttcag
     7261 cagttccagg gcgctgttga tcactttgct cttgtccagc cggctcatgt cgacattctt
     7321 tgccaaaatg atgagacagc acaataacca gcacgttgcc caggagctgt aggaaaaaga
     7381 agaaggcatg aacatggtta gcagaggctc tagagccgcc ggtcacacgc cagaagccga
     7441 accccgccct gccccgtccc ccccgaaggc agccgtcccc ccgcggacag ccccgaggct
     7501 ggagagggag aaggggacgg cggcgcggcg acgcacgaag gccctccccg cccatttcct
     7561 tcctgccggc gccgcaccgc ttcgccccgc gcccgctaga gggggtgcgg cggcgcctcc
     7621 cagatttcgg ctccgcacag atttgggaca aaggaagtcc ctgcgccctc tcgcacgatt
     7681 accataaaag gcaatggctg cggctcgccg cgcctcgaca gccgccggcg ctccgggggc
     7741 cgccgcgccc ctcccccgag ccctccccgg cccgaggcgg ccccgccccg cccggcaccc
     7801 ccacctgccg ccaccccccg cccggcacgg cgagccccgc gccacgcccc gtacggagcc
     7861 ccgcacccga agccgggccg tgctcagcaa ctcggggagg ggggtgcagg gggggttgca
     7921 gcccgaccga cgcgcccaca ccccctgctc acccccccac gcacacaccc cgcacgcagc
     7981 ctttgttccc ctcgcagccc cccccgcacc gcggggcacc gcccccggcc gcgctcccct
     8041 cgcgcacact gcggagcgca caaagccccg cgccgcgccc gcagcgctca cagccgccgg
     8101 gcagcgcgga gccgcacgcg gcgctcccca cgcacacaca cacgcacgca ccccccgagc
     8161 cgctcccccc gcacaaaggg ccctcccgga gcccctcaag gctttcacgc agccacagaa
     8221 aagaaacaag ccgtcattaa accaagcgct aattacagcc cggaggagaa gggccgtccc
     8281 gcccgctcac ctgtgggagt aacgcggtca gtcagagccg gggcgggcgg cgcgaggcgg
     8341 cggcggagcg gggcacgggg cgaaggcagc gtcgcagcga ctccccgccc gccgcgcgct
     8401 tcgcttttta tagggccgcc gccgccgccg cctcgccata aaaggaaact ttcggagcgc
     8461 gccgctctga ttggctgccg ccgcacctct ccgcctcgcc ccgccccgcc cctcgccccg
     8521 ccccgccccg cctggcgcgc gccccccccc cccccccgcc cccatcgctg cacaaaataa
     8581 ttaaaaaata aataaataca aaattggggg tggggagggg ggggagatgg ggagagtgaa
     8641 gcagaacgtg gggctcacct cgaccatggt aatagcgatg actaatacgt agatgtactg
     8701 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
     8761 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
     8821 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
     8881 tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
     8941 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
     9001 ccccgtaatt gattactatt aataactagt caataatcaa tgtcaataaa atatctttat
     9061 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc
     9121 atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag
     9181 gtgccagaac atttctctga gctcacgggg acagcccccc cccaaagccc ccagggatgt
     9241 aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc
     9301 ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg
     9361 tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg
     9421 gggggatacg gggaaaaagc tttaggctga aagagagatt tagaatgaca gaatcataga
     9481 acggcctggg ttgcaaagga gcacagtgct catccagatc caaccccctg ctatgtgcag
     9541 ggtcatcaac cagcagccca ggctgcccag agccacatcc agcctggcct tgaatgcctg
     9601 cagggatggg gcatccacag cctccttggg caacctgttc agtgcgtcac caccctctgg
     9661 gggaaaaact gcctcctcat atccaaccca aacctcccct gtctcagtgt aaagccattc
     9721 ccccttgtcc tatcaagggg gagtttgctg tgacattgtt ggtctggggt gacacatgtt
     9781 tgccaattca gtgcatcacg gagaggcaga tcttggggat aaggaagtgc aggacagcat
     9841 ggacgtggga catgcaggtg ttgagggctc tgggacactc tccaagtcac agcgttcaga
     9901 acagccttaa ggataagaag ataggataga aggacaaaga gcaagttaaa acccagcatg
     9961 gagaggagca caaaaaggcc acagacactg ctggtccctg tgtctgagcc tgcatgtttg
    10021 atggtgtctg gatgcaagca gaaggggtgg aagagcttgc ctggagagat acagctgggt
    10081 cagtaggact gggacaggca gctggagaat tgccatgtag atgttcatac aatcgtcaaa
    10141 tcatgaaggc tggaaaagcc ctccaagatc cccaagacca accccaaccc acccaccgtg
    10201 cccactggcc atgtccctca gtgccacatc cccacagttc ttcatcacct ccagggacgg
    10261 tgaccccccc acctccgtgg gcagctgtgc cactgcagca ccgctctttg gagaaggtaa
    10321 atcttgctaa atccagcccg accctcccct ggcacaacgt aaggccatta tctctcatcc
    10381 aactccagga cggagtcagt gagaatatta taacttcgta tagcatacat tatacgaagt
    10441 tatgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
    10501 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
    10561 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
    10621 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
    10681 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
    10741 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
    10801 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
    10861 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
    10921 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
    10981 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
    11041 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
    11101 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
    11161 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
    11221 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
    11281 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
    11341 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgcgctttt
    11401 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
    11461 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
    11521 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
    11581 ttcttccatt tcaggtgtcg tgatttaaat cccaccatga ccgagtacaa gcccacggtg
    11641 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
    11701 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
    11761 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
    11821 gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
    11881 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
    11941 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
    12001 ggagtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
    12061 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
    12121 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
    12181 cgcacctggt gcatgacccg caagcccggt gccggatcgg gagagggcag aggaagtctg
    12241 ctaacatgcg gtgacgtcga ggagaatcct ggcccaccgg tccctgccgc taaacgggtc
    12301 aaactcgacg gcggttctgg aggtcctgct gcaaagcgcg ttaagttgga tggagggtca
    12361 gggggagcca ccgccaccat ggtgtctaag ggcgaagagc tgattaagga gaacatgcac
    12421 atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc
    12481 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct
    12541 ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc
    12601 aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg
    12661 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
    12721 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc
    12781 cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct
    12841 gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg
    12901 atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct
    12961 ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa cgagacctac
    13021 gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac
    13081 aagcttaatt aggcggccgc taagaattct gtgccttcta gttgccagcc atctgttgtt
    13141 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
    13201 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
    13261 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
    13321 gtgggctcta tggataactt cgtatagcat acattatacg aagttatttg gtatggcttt
    13381 ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa
    13441 agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga
    13501 tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg
    13561 gggagggacg taattacatc cctgggggct ttgggggggg gctgtccctg attctataac
    13621 aagaaaatat atatataata agttatcacg taagtagaac atgaaataac aatataatta
    13681 tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt ttgactcacg
    13741 cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc ctcacgggag
    13801 ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct atttagaaag
    13861 agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt tctagggtta
    13921 atctagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca tcgcccaagc
    13981 tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga ggttgaagcg
    14041 gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg aaaacgcacg
    14101 tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga actgttcgtt
    14161 caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg gatggtcagc
    14221 ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg tgccggtgtg
    14281 cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg gtatcctggc
    14341 tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg cgcttggcgt
    14401 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    14461 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    14521 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    14581 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    14641 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    14701 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    14761 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    14821 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    14881 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    14941 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    15001 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    15061 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    15121 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    15181 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    15241 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    15301 gagttgg
//