LOCUS       pTipi2.1-AZU_SP         3897 bp    DNA     circular UNA 26-APR-2023
DEFINITION  .
ACCESSION   urn.local...g-i2b0ih8
VERSION     urn.local...g-i2b0ih8
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     Regulatory      149..528
                     /created_by="User"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CMV_PDL1_WPRE_HSV-TK"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; CMV
                     enhancer (<a
                     href=""urn:local:.:81-fx0r57g"">CMV_PDL1_WPRE_HSV-TK</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV enhancer (<a
                     href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV enhancer (<a
                     href=""urn:local:.:7n-fx0qeo4"">4_pTipi1_1_ORI</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; CMV
                     enhancer (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; CMV
                     enhancer (<a
                     href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV_enhancer (<a
                     href=""urn:local:.:1o-g653e4n"">IPI0032139H_19_238_NS_spri
                     nt7</a>)"
                     /label="CMV enhancer"
     promoter        150..726
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CMV_PDL1_WPRE_HSV-TK"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV_immearly_promoter (<a
                     href=""urn:local:.:81-fx0r57g"">CMV_PDL1_WPRE_HSV-TK</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV_immearly_promoter (<a
                     href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV_immearly_promoter (<a
                     href=""urn:local:.:7n-fx0qeo4"">4_pTipi1_1_ORI</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV_immearly_promoter (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV_immearly_promoter (<a
                     href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:379/6;
                     CMV_promoter (<a
                     href=""urn:local:.:1o-g653e4n"">IPI0032139H_19_238_NS_spri
                     nt7</a>)"
                     /label="CMV_immearly_promoter"
     promoter        696..815
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CMV_PDL1_WPRE_HSV-TK"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV2_promoter (<a
                     href=""urn:local:.:81-fx0r57g"">CMV_PDL1_WPRE_HSV-TK</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV2_promoter (<a
                     href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV2_promoter (<a
                     href=""urn:local:.:7n-fx0qeo4"">4_pTipi1_1_ORI</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV2_promoter (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     CMV2_promoter (<a
                     href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /label="CMV2_promoter"
     intron          828..960
                     /note="/vntifkey=15"
                     /note="Derived using Geneious Prime 2021.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CMV_PDL1_WPRE_HSV-TK"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; beta
                     globin/IgG chimaeric intron (<a
                     href=""urn:local:.:81-fx0r57g"">CMV_PDL1_WPRE_HSV-TK</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     beta globin/IgG chimaeric intron (<a
                     href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     beta globin/IgG chimaeric intron (<a
                     href=""urn:local:.:7n-fx0qeo4"">4_pTipi1_1_ORI</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; beta
                     globin/IgG chimaeric intron (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; beta
                     globin/IgG chimaeric intron (<a
                     href=""urn:local:.:yw-g6w6vhi"">MCS_design_w_intron_w_AZU1
                     _SP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; beta
                     globin/IgG chimaeric intron (<a
                     href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; beta
                     globin/IgG chimaeric intron (<a
                     href=""urn:local:.:xk-g6vvcqy"">PDL_fragement_with_Intron_
                     MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; beta
                     globin/IgG chimaeric intron (<a
                     href=""urn:local:.:x3-g6vtng2"">MCS_design_w_intron</a>)"
                     /label="beta globin/IgG chimaeric intron"
     sig_peptide     995..1051
                     /created_by="Haisun"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CMV_PDL1_WPRE_HSV-TK"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Human
                     Azurocidin SP (<a
                     href=""urn:local:.:81-fx0r57g"">CMV_PDL1_WPRE_HSV-TK</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Human Azurocidin SP (<a
                     href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Human Azurocidin SP (<a
                     href=""urn:local:.:7n-fx0qeo4"">4_pTipi1_1_ORI</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Human
                     Azurocidin SP (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Human Azurocidin SP (<a
                     href=""urn:local:.:yw-g6w6vhi"">MCS_design_w_intron_w_AZU1
                     _SP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Human Azurocidin SP (<a
                     href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /label="Human Azurocidin SP"
     regulatory      1088..1676
                     /created_by="Haisun"
                     /note="Derived using Geneious Prime 2021.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CMV_PDL1_WPRE_HSV-TK"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; WPRE
                     (<a
                     href=""urn:local:.:81-fx0r57g"">CMV_PDL1_WPRE_HSV-TK</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     WPRE (<a
                     href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     WPRE (<a
                     href=""urn:local:.:7n-fx0qeo4"">4_pTipi1_1_ORI</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; WPRE (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; WPRE
                     (<a href=""urn:local:.:y-g61avr6"">3_pTipi3-PDL1 -
                     WPRE</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; WPRE
                     (<a href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; WPRE
                     (<a
                     href=""urn:local:.:1o-g653e4n"">IPI0032139H_19_238_NS_spri
                     nt7</a>)"
                     /label="WPRE"
     polyA_signal    1742..1790
                     /note="HSV TK poly(A) signal"
                     /note="herpesvirus thymidine kinase polyadenylation
                     signal"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CMV_PDL1_WPRE_HSV-TK"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; HSV TK
                     poly(A) signal polyA signal (<a
                     href=""urn:local:.:81-fx0r57g"">CMV_PDL1_WPRE_HSV-TK</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     HSV TK poly(A) signal polyA signal (<a
                     href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     HSV TK poly(A) signal polyA signal (<a
                     href=""urn:local:.:7n-fx0qeo4"">4_pTipi1_1_ORI</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; HSV TK
                     poly(A) signal polyA signal (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; HSV
                     TK poly(A) signal polyA signal (<a
                     href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     HSV_TK_polyA (<a
                     href=""urn:local:.:1o-g653e4n"">IPI0032139H_19_238_NS_spri
                     nt7</a>)"
                     /label="HSV TK poly(A) signal polyA signal"
     rep_origin      complement(2019..2692)
                     /created_by="User"
                     /X-Transferred_From="pUC57"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC
                     origin (<a href=""urn:local:.:6i-fx0oc63"">pUC57</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pUC origin (<a href=""urn:local:.:6m-fx0ogeq"">pUC18</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC
                     origin (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pUC
                     origin (<a href=""urn:local:.:1j-g65390z"">pUC18</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pUC
                     origin (<a
                     href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /label="pUC origin"
     CDS             complement(2837..3697)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="2_pTipi1_1_AMP"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     (<a href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/3;
                     AmpR (<a href=""urn:local:.:6i-fx0oc63"">pUC57</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/3;
                     AmpR (<a href=""urn:local:.:6m-fx0ogeq"">pUC18</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     (<a href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/3; AmpR
                     (<a href=""urn:local:.:1j-g65390z"">pUC18</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/3; AmpR
                     (<a
                     href=""urn:local:.:1o-g653e4n"">IPI0032139H_19_238_NS_spri
                     nt7</a>)"
                     /label="AmpR"
     Promoter        complement(3698..3802)
                     /created_by="User"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pUC57"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:6i-fx0oc63"">pUC57</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:7m-fx0qeo4"">2_pTipi1_1_AMP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:6m-fx0ogeq"">pUC18</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR promoter (<a
                     href=""urn:local:.:7n-fx0qeo4"">4_pTipi1_1_ORI</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2_pTipi2-PDL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:1t-g653u67"">2_pTipi2-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:1j-g65390z"">pUC18</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:1e-g6535n4"">3_pTipi3-PDL1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:1o-g653e4n"">IPI0032139H_19_238_NS_spri
                     nt7</a>)"
                     /label="AmpR promoter"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactaga cattgattat tgactagtta ttaatagtaa
      181 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      241 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      301 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      361 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      421 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      481 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      541 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      661 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      721 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      781 gacctccata gaagacaccg ggaccgatcc agcctccgga cctgcaggta agtatcaagg
      841 ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct
      901 tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag
      961 gtcgactagt gaattctcta gaggtaccgc caccatgacc cggctgacag tcctggccct
     1021 gctggctggt ctgctggcgt cctcgagggc caagcttgct agcggccgcg agctctgata
     1081 tctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1141 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     1201 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1261 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     1321 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     1381 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     1441 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     1501 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     1561 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     1621 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     1681 aaacggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     1741 acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc attgcgttgc
     1801 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     1861 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     1921 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1981 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2041 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2101 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2161 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2221 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2281 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2341 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2401 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2461 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2521 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2581 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2641 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2701 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2761 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2821 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2881 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2941 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3001 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3061 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3121 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3181 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3241 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3301 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3361 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3421 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3481 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3541 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3601 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3661 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3721 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3781 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     3841 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
