LOCUS       Exported                2847 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local.rgkiwi.au-7wnclot
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      589
                     /label=ORI
     primer_bind     689..708
                     /label=G00101
     misc_feature    complement(815..840)
                     /label=RJ_00163Ra
     protein_bind    830..835
                     /label=BsaI Bind
     misc_feature    830..835
                     /label=BsaI
     misc_feature    837..840
                     /label=pL0 3-T
                     /label=nonstandard type:  Gg_overhang
     misc_feature    complement(843..848)
                     /label=BbsI
     misc_feature    complement(843..848)
                     /label=BpiI
     misc_feature    884..1007
                     /label=Plac
     misc_difference 972
                     /label=G-->A
     CDS             1014..1337
                     /label=LacZ
     misc_feature    1055..1060
                     /label=XbaI
     misc_feature    1061..1066
                     /label=BamHI
     misc_feature    1082..1087
                     /label=EcoRI
     misc_difference 1356
                     /label=T-->C
     misc_feature    1427..1432
                     /label=BpiI
     misc_feature    1427..1432
                     /label=BbsI
     primer_bind     1435..1454
                     /label=RJ_00163Fa
     misc_feature    1435..1438
                     /label=pL0 T
                     /label=nonstandard type:  Gg_overhang
     misc_feature    complement(1440..1445)
                     /label=BsaI
     protein_bind    complement(1440..1445)
                     /label=BsaI Bind
     misc_feature    1595..1618
                     /label=Pamp
     misc_feature    complement(1610..1630)
                     /label=JH856R
     CDS             1665..2677
                     /label=Sm/Sp no DraIII (Streptomycin/SpectinomycinResi
                     /label=Sm/Sp no DraIII 
                     (Streptomycin/SpectinomycinResistance)
     misc_feature    complement(2138..2161)
                     /label=RJ_00189Ra
     primer_bind     2147..2167
                     /label=RJ_00190Fa
     rep_origin      complement(join(2772..2847,1..607))
                     /direction=LEFT
                     /label=ColE1 Origin
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcacaac
      841 ttgtcttcgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      901 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      961 tcgtatgttg tatggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     1021 tgattacgcc aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct
     1081 cgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     1141 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     1201 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     1261 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     1321 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     1381 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgacgaag acaacgcttg
     1441 agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg
     1501 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     1561 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1621 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact
     1681 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     1741 gttatgactg tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     1801 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     1861 tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca
     1921 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     1981 tttgtacggc tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt
     2041 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     2101 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     2161 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     2221 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     2281 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     2341 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     2401 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     2461 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     2521 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     2581 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta
     2641 cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata
     2701 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2761 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2821 ccccgtagaa aagatcaaag gatcttc
//
