LOCUS       Exported                3105 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local.rgkiwi.7s-7wn7xzp
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 96 documents from 
            Done.gb [pL0-P.2] CMV(from pRJ_00025). ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(4..9)
                     /label=BBb scar
     rep_origin      589
                     /label=ORI
     primer_bind     689..708
                     /label=G00101
     primer_bind     808..835
                     /label=fwd
     misc_feature    complement(815..840)
                     /label=RJ_00148Ra
     protein_bind    830..835
                     /label=BsaI Bind
     misc_feature    830..835
                     /label=BsaI
     misc_feature    837..840
                     /label=pL0 I-P
                     /label=nonstandard type:  gg_overhang
     promoter        914..1692
                     /label=CMV IE Promoter
     enhancer        1065..1471
                     /label=CMV IE Enhancer
     protein_bind    complement(1104..1109)
                     /label=SP1 Binding Site
     protein_bind    complement(1118..1123)
                     /label=SP1 Binding Site
     protein_bind    1126..1133
                     /label=CREB Response Element (CRE)
     protein_bind    1167..1176
                     /label=NFkB Binding Site
     protein_bind    1179..1186
                     /label=CREB Response Element (CRE)
     protein_bind    complement(1251..1256)
                     /label=SP1 Binding Site
     protein_bind    1262..1269
                     /label=CREB Response Element (CRE)
     protein_bind    1318..1327
                     /label=NFkB Binding Site
     promoter        1393..1397
                     /label=CCAAT Box
     protein_bind    1415..1421
                     /label=AP-1 Binding Site
     protein_bind    1423..1432
                     /label=NFkB Binding Site
     protein_bind    1448..1455
                     /label=CREB Response Element (CRE)
     promoter        1474..1598
                     /label=Minimal Promoter
     protein_bind    1486..1495
                     /label=NFkB Binding Site
     protein_bind    complement(1513..1518)
                     /label=SP1 Binding Site
     protein_bind    1532..1537
                     /label=SP1 Binding Site
     promoter        1560..1566
                     /label=TATA Box
     promoter        1589..1595
                     /label=Initiator
     misc_RNA        1591
                     /label=+1 Site
     misc_feature    1600
                     /label=MSC 1
                     /label=MSC\1
     promoter        1626..1630
                     /label=DPE
     primer_bind     1693..1712
                     /label=RJ_00148Fa
     misc_feature    1693..1696
                     /label=pL0 P-5 v2
                     /label=nonstandard type:  gg_overhang
     protein_bind    complement(1698..1703)
                     /label=BsaI Bind
     misc_feature    complement(1698..1703)
                     /label=BsaI
     misc_feature    1853..1876
                     /label=Pamp
     misc_feature    complement(1868..1888)
                     /label=JH856R
     misc_feature    1923..2081
                     /label=Spec
     misc_feature    2623..2628
                     /label=BBb scar
     misc_feature    3000..3005
                     /label=BBb scar
     misc_feature    complement(3012..3017)
                     /label=BBb scar
     rep_origin      complement(join(3030..3105,1..607))
                     /direction=LEFT
                     /label=ColE1 Origin
     misc_feature    3098..3103
                     /label=BBb scar
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcatact
      841 ggcctaactg gcctcaatat tggccattag ccatattatt cattggttat atagcataaa
      901 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      961 ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt tattaatagt
     1021 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     1081 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     1141 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     1201 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
     1261 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
     1321 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     1381 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     1441 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     1501 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     1561 atataagcag agctcgttta gtgaaccgtc agatcactag aagctttatt gcggtagttt
     1621 atcacagtta aattgctaac gcagtcagtg ggcctcggcg gccaagcttg gcaatccggt
     1681 actgttggta aacagatgag accacgaagt ggctcttcag tggacgaaag ggcctcgtga
     1741 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1801 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1861 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1921 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     1981 tggcggtttt catggcttgt tatgactgtt tttttttggg gtacagtcta tgcctcgggc
     2041 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     2101 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat
     2161 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     2221 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     2281 cgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     2341 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     2401 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     2461 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     2521 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     2581 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     2641 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     2701 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     2761 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     2821 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     2881 ggaagaattt gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc
     2941 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3001 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3061 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
