LOCUS       Exported                2570 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  [pL0-P.2] TRE3G.
ACCESSION   urn.local.jones.tji-8qj5swr
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: pBjh_5042.gb 
            ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(4..9)
                     /label=BBb scar
                     /label=BBb scar(4)
     rep_origin      589
                     /label=ORI
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pBjh_3369"
                     /note="/X-Transferred_Similarity=100.00%"
     primer_bind     689..708
                     /label=G00101
     protein_bind    830..835
                     /label=BsaI Bind
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    830..835
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
                     /note="/annotation_group=GGTCTC"
     promoter        837..1161
                     /label=TRE3G
                     /note="/created_by=User"
     misc_feature    837..840
                     /label=pL0 I-P
                     /label=nonstandard type:  gg_overhang
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    837..840
                     /label=Ligation
                     /label=nonstandard type:  ligation
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pBjh_3369"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    841..846
                     /label=XhoI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^TCGAG"
                     /note="/annotation_group=CTCGAG"
     enhancer        852..871
                     /label=TetO
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pBjh_3369"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     enhancer        888..907
                     /label=Tet)
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pBjh_3369"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     enhancer        924..943
                     /label=TetO
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pBjh_3369"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     enhancer        960..979
                     /label=TetO
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pBjh_3369"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     enhancer        996..1015
                     /label=TetO
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pBjh_3369"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     enhancer        1032..1051
                     /label=TetO
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pBjh_3369"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     enhancer        1068..1087
                     /label=TetO
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pBjh_3369"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    1090..1094
                     /label=TATA-like
                     /note="/created_by=jones"
     promoter        1096..1155
                     /label=Minimal CMV
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pBjh_3369"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=jones"
     promoter        1113..1119
                     /label=BRE Box
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pBjh_3369"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     promoter        1120..1126
                     /label=TATA Box
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pBjh_3369"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     promoter        1147..1151
                     /label=Possible Double TSS
                     /note="/created_by=User"
     misc_RNA        1148
                     /label=Possible TSS
                     /note="/created_by=User"
                     /note="/modified_by=User"
     promoter        1149..1155
                     /label=Initiator
                     /note="/created_by=User"
     misc_RNA        1151
                     /label=Putative +1 Site
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    1158..1161
                     /label=pL0 P-5 v2
                     /label=nonstandard type:  gg_overhang
                     /note="/created_by=User"
     misc_feature    complement(1163..1168)
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
                     /note="/annotation_group=GGTCTC"
     protein_bind    complement(1163..1168)
                     /label=BsaI Bind
                     /note="/created_by=User"
     misc_feature    1318..1341
                     /label=Pamp
                     /note="Geneious type: promoter_prokaryotic"
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(1333..1353)
                     /label=JH856R
     misc_feature    1388..1546
                     /label=Spec
     primer_bind     complement(1861..1884)
                     /label=RJ_00189Ra
     primer_bind     1870..1890
                     /label=RJ_00190Fa
     misc_feature    2465..2470
                     /label=BBb scar
                     /label=BBb scar(1)
     misc_feature    complement(2477..2482)
                     /label=BBb scar
                     /label=BBb scar(3)
     rep_origin      complement(join(2495..2570,1..607))
                     /direction=LEFT
                     /label=ColE1 origin
     misc_feature    2563..2568
                     /label=BBb scar
                     /label=BBb scar(2)
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcatact
      841 ctcgagttta ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga
      901 tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact
      961 ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat
     1021 ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga
     1081 tagagaacgt ataagcttta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta
     1141 gtgaaccgtc agatcgccag atgagaccac gaagtggctc ttcagtggac gaaagggcct
     1201 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     1261 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1321 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1381 gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     1441 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ttggggtaca gtctatgcct
     1501 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     1561 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg
     1621 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     1681 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     1741 cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     1801 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     1861 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     1921 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     1981 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     2041 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     2101 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     2161 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     2221 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     2281 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     2341 cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa
     2401 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2461 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2521 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
