LOCUS       Exported                2511 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: benchling_export 
            (6).gb Concatenation of 6 sequences[pL0-P.2] 
            6xGal4-minCMV53-1xBM3R1Concatenation of 6 sequences
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(589)
                     /direction=LEFT
                     /label=ORI
     primer_bind     689..708
                     /label=G00101
                     /note="created by: User Sequence: ATTACCGCCTTTGAGTGAGC"
     protein_bind    830..835
                     /label=BsaI Bind
                     /note="created by: User"
     misc_feature    837..840
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    837..840
                     /label=pL0 I-P
                     /label=nonstandard type:  Gg_overhang
                     /note="created by: User"
     promoter        841..862
                     /label=No TSS
                     /note="created by: User modified by: User"
     misc_feature    863..866
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    863..866
                     /label=pSub0-P S5
                     /label=nonstandard type:  Gg_overhang
                     /note="created by: User modified by: User"
     misc_feature    867..870
                     /label=Insert L
                     /label=nonstandard type:  Gg_overhang
                     /note="created by: User"
     enhancer        871..931
                     /label=Gal4 Responsive Element
                     /note="created by: User modified by: User"
     protein_bind    871..887
                     /label=Gal4 Bind
                     /note="created by: User modified by: User"
     protein_bind    893..909
                     /label=Gal4 Bind
                     /note="created by: User modified by: User"
     protein_bind    915..931
                     /label=Gal4 Bind
                     /note="created by: User modified by: User"
     misc_feature    932..935
                     /label=Insert R
                     /label=nonstandard type:  Gg_overhang
                     /note="created by: User"
     misc_feature    936..939
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    936..939
                     /label=pSub0-P S4
                     /label=nonstandard type:  Gg_overhang
                     /note="created by: User"
     misc_feature    940..943
                     /label=Insert L
                     /label=nonstandard type:  Gg_overhang
                     /note="created by: User"
     enhancer        944..1004
                     /label=Gal4 Responsive Element
                     /note="created by: User modified by: User"
     protein_bind    944..960
                     /label=Gal4 Bind
                     /note="created by: User modified by: User"
     protein_bind    966..982
                     /label=Gal4 Bind
                     /note="created by: User modified by: User"
     protein_bind    988..1004
                     /label=Gal4 Bind
                     /note="created by: User modified by: User"
     misc_feature    1005..1008
                     /label=Insert R
                     /label=nonstandard type:  Gg_overhang
                     /note="created by: User"
     misc_feature    1009..1012
                     /label=pSub0-P S3
                     /label=nonstandard type:  Gg_overhang
                     /note="created by: User modified by: User"
     misc_feature    1009..1012
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        1013..1074
                     /label=CMV53
                     /note="created by: User modified by: User"
     protein_bind    1032..1037
                     /label=SP1 Bind (Short)
                     /note="created by: User modified by: User"
     promoter        1053..1059
                     /label=BRE-Like
                     /note="created by: User"
     promoter        1060..1066
                     /label=TATA Box
                     /note="created by: User"
     misc_feature    1067..1074
                     /label=5' GC-Rich
                     /label=nonstandard type:  Compositiona...
                     /note="created by: User"
     misc_feature    1075..1078
                     /label=pSub0-P S2
                     /label=nonstandard type:  Gg_overhang
                     /note="created by: User modified by: User"
     misc_feature    1075..1078
                     /label=Ligation
                     /label=nonstandard type:  ligation
     promoter        1079..1098
                     /label=3xBM3R1 / Inr
                     /note="created by: User modified by: User"
     misc_feature    1079..1098
                     /label=BM3R1
                     /label=nonstandard type:  repressor
                     /note="ApEinfo_label: New Feature ApEinfo_fwdcolor: cyan 
                     ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: 
                     misc_feature modified by: User"
     misc_RNA        1089
                     /label=Putative +1 Site
                     /note="created by: User"
     promoter        1091..1097
                     /label=Initiator
                     /note="ApEinfo_fwdcolor: #ffff00 ApEinfo_revcolor: green 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 NCBI Feature Key: misc_feature modified 
                     by: User"
     misc_RNA        1093
                     /label=Possible TSS
                     /note="created by: User modified by: User"
     misc_feature    1099..1102
                     /label=pL0 P-5 v2
                     /label=nonstandard type:  Gg_overhang
                     /note="created by: User modified by: User"
     misc_feature    1099..1102
                     /label=Ligation
                     /label=nonstandard type:  ligation
     protein_bind    complement(1104..1109)
                     /label=BsaI Bind
                     /note="created by: User"
     misc_feature    1259..1282
                     /label=Pamp
                     /label=nonstandard type:  Promoter_pro...
                     /note="created by: User"
     misc_feature    complement(1274..1294)
                     /label=JH856R
                     /label=nonstandard type:  primer_bind_...
                     /note="Mismatches: 0 created by: primer3 annotation group: 
                     434056799628541fe881553-2567-4259-aa41-255f88418749 %GC: 
                     47.6 Tm: 56.4 Hairpin Tm: None Self Dimer Tm: 13.2 
                     Sequence: CAGGGTTATTGTCTCATGAGC"
     misc_feature    1329..2341
                     /label=Spec
     misc_feature    complement(1802..1825)
                     /label=RJ_00189Ra
                     /label=nonstandard type:  primer_bind_...
                     /note="Mismatches: 0 created by: primer3 annotation group: 
                     233147216235674951871e59-4f03-4619-a496-ce533ac81289 %GC: 
                     45.8 Tm: 60.3 Hairpin Tm: 36.5 Self Dimer Tm: 4.8 Sequence:
                     CACAACAATGGTGACTTCTACAGC"
     primer_bind     1811..1831
                     /label=RJ_00190Fa
                     /note="Mismatches: 0 created by: primer3 annotation group: 
                     2331472254687687160461dd-ffcf-413c-a17d-638a958fb904 %GC: 
                     52.4 Tm: 60.6 Hairpin Tm: None Self Dimer Tm: 27.4 
                     Sequence: GTCACCATTGTTGTGCACGAC"
     rep_origin      complement(join(2436..2511,1..607))
                     /direction=LEFT
                     /label=ColE1 Origin
                     /note="created by: User"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcatact
      841 ggttcctcct ctctctcctt cttgaggtat cggatcgcca ttcgccgacg cccggagcac
      901 tatgcaccga agctcggatt gcttcgcacc ggaagcctag tatcggatcg ccattcgccg
      961 acgcccggag cactatgcac cgaagctcgg attgcttcgc accggaaggt accaacaaaa
     1021 tgtcgtaaca agggcggtag gcgtgtacgg tgggaggtct atataagcag agctccagcg
     1081 gaatgaacgt tcattccgca gatgagacca cgaagtggct cttcagtgga cgaaagggcc
     1141 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     1201 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1261 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1321 ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     1381 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc
     1441 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     1501 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     1561 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     1621 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     1681 acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     1741 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     1801 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     1861 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     1921 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     1981 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2041 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2101 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     2161 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     2221 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     2281 tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata
     2341 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2401 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2461 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
