LOCUS       Exported                2274 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local.jeremy.9te-8qo9bzz
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: pL0-5.2 1x-uORF 
            weak.gb Concatenation of 2 sequences.
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      589
                     /label=ORI
     primer_bind     689..708
                     /label=KK_G00101
     misc_feature    complement(809..836)
                     /label=rev 1
                     /note="Sequence: TGAGACCACAGAGTGATTAATGAATCGG Hairpin_Tm: 
                     43.9 Tm: 62.9 Self_Dimer_Tm: None %GC: 42.9 modified_by: 
                     User note: Geneious type: primer_bind_reverse"
     misc_feature    830..835
                     /label=BsaI
                     /note="Recognition_pattern: GGTCTC(1/5) annotation_group: 
                     GGTCTC note: Geneious type: restriction site"
     misc_feature    837..840
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    841..861
                     /label=Digestion of 13.56 - Fragment 2 extraction(conc
                     /label=Digestion of 13.56 - Fragment 2 
                     extraction(concatenated sequence 2)
                     /note="Source: 
                     referenceToDocument:urn:local:jeremy:9tc-8qo9bzz note: 
                     Geneious type: Concatenated sequence"
     misc_feature    851..862
                     /label=uORF x1 weak
                     /note="created_by: User modified_by: User"
     misc_feature    862..865
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    join(866..2274,1..836)
                     /label=Digestion of p_5' UTR (JGv2) - Fragment 2(conca
                     /label=Digestion of p_5' UTR (JGv2) - Fragment 
                     2(concatenated sequence 1)
                     /note="note: Geneious type: Concatenated sequence"
     misc_feature    complement(867..872)
                     /label=BsaI
                     /note="Recognition_pattern: GGTCTC(1/5) annotation_group: 
                     GGTCTC note: Geneious type: restriction site"
     primer_bind     complement(894..912)
                     /label=KK_lvl0Rev
     misc_feature    1092..1250
                     /label=Spec
     misc_feature    join(2264..2274,1..604)
                     /label=rep (pMB1)
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcacaga
      841 taagtttgta cttatgggtt gaggttgaga ccacgaagtg gctcttcagt ggacgaaagg
      901 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      961 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1021 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1081 aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     1141 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttttgggg tacagtctat
     1201 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     1261 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga
     1321 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     1381 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     1441 gccacacagc gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     1501 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     1561 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     1621 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     1681 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     1741 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     1801 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     1861 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     1921 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     1981 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     2041 agatcagttg gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa
     2101 ataactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     2161 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     2221 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
