LOCUS       Exported                2286 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local.jeremy.9s3-8qo9bzk
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: pL0-5.2 2x-uORF.gb 
            Concatenation of 2 sequences.
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      589
                     /label=ORI
     misc_feature    complement(809..836)
                     /label=rev 1
                     /note="Sequence: TGAGACCACAGAGTGATTAATGAATCGG Hairpin_Tm: 
                     43.9 Tm: 62.9 Self_Dimer_Tm: None %GC: 42.9 modified_by: 
                     User note: Geneious type: primer_bind_reverse"
     misc_feature    830..835
                     /label=BsaI
                     /note="Recognition_pattern: GGTCTC(1/5) annotation_group: 
                     GGTCTC note: Geneious type: restriction site"
     misc_feature    837..840
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    841..873
                     /label=Digestion of 13.58 - Fragment 2 extraction(conc
                     /label=Digestion of 13.58 - Fragment 2 
                     extraction(concatenated sequence 2)
                     /note="Source: 
                     referenceToDocument:urn:local:jeremy:9s1-8qo9bzk note: 
                     Geneious type: Concatenated sequence"
     misc_feature    851..874
                     /label=uORF x2
                     /note="created_by: User modified_by: User"
     misc_feature    874..877
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    join(878..2286,1..836)
                     /label=Digestion of p_5' UTR (JGv2) - Fragment 2(conca
                     /label=Digestion of p_5' UTR (JGv2) - Fragment 
                     2(concatenated sequence 1)
                     /note="note: Geneious type: Concatenated sequence"
     misc_feature    complement(879..884)
                     /label=BsaI
                     /note="Recognition_pattern: GGTCTC(1/5) annotation_group: 
                     GGTCTC note: Geneious type: restriction site"
     misc_feature    1104..1262
                     /label=Spec Truncated
     misc_feature    join(2276..2286,1..604)
                     /label=rep (pMB1)
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcacaga
      841 taagtttgta accatgggtt gaaccatggg ttgaggttga gaccacgaag tggctcttca
      901 gtggacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      961 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1021 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1081 aataatattg aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1141 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttttgg
     1201 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     1261 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     1321 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     1381 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     1441 tggcggcctg aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga
     1501 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     1561 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     1621 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     1681 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     1741 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     1801 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     1861 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     1921 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     1981 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     2041 ggcctcgcgc gcagatcagt tggaagaatt tgtccattac gtaaaaggcg agatcaccaa
     2101 ggtagtcggc aaataactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2161 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2221 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2281 atcttc
//
