LOCUS       Exported                2298 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local.jeremy.9sm-8qo9bzs
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: pL0-5.2 3x uORF.gb 
            Concatenation of 2 sequences.
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      590
                     /label=ORI
     misc_feature    complement(810..837)
                     /label=rev 1
                     /note="Sequence: TGAGACCACAGAGTGATTAATGAATCGG Hairpin_Tm: 
                     43.9 Tm: 62.9 Self_Dimer_Tm: None %GC: 42.9 modified_by: 
                     User note: Geneious type: primer_bind_reverse"
     misc_feature    831..836
                     /label=BsaI
                     /note="Recognition_pattern: GGTCTC(1/5) annotation_group: 
                     GGTCTC note: Geneious type: restriction site"
     misc_feature    838..841
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    842..886
                     /label=Digestion of 13.59 - Fragment 2 extraction(conc
                     /label=Digestion of 13.59 - Fragment 2 
                     extraction(concatenated sequence 2)
                     /note="Source: 
                     referenceToDocument:urn:local:jeremy:9sk-8qo9bzr note: 
                     Geneious type: Concatenated sequence"
     misc_feature    852..887
                     /label=uORF x3
                     /note="created_by: User modified_by: User"
     misc_feature    887..890
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    join(891..2298,1..837)
                     /label=Digestion of p_5' UTR (JGv2) - Fragment 2(conca
                     /label=Digestion of p_5' UTR (JGv2) - Fragment 
                     2(concatenated sequence 1)
                     /note="note: Geneious type: Concatenated sequence"
     misc_feature    complement(892..897)
                     /label=BsaI
                     /note="Recognition_pattern: GGTCTC(1/5) annotation_group: 
                     GGTCTC note: Geneious type: restriction site"
     CDS             1117..2129
                     /label=Sm/Sp no DraIII
     CDS             1117..2129
                     /codon_start=1
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFLGYSLCLGHPSSKR
                     VTPWVDV*CYGAATMLRSRAVALKQS*TS*GKR*SPKYRLNYQR*LASSSAISNRRCWP
                     YICTAPQWMAA*SHTAILICWLR*P*GLMKQRGEL*STTFWKLRLPLERARFSAL*KSP
                     LLCTTTSFRGVIQLSANCNLENGSAMTFLQVSSSQPRSTLIWLSC*QKQENIALPW*VQ
                     RRRNSLIRFLNRIYLRR*MKP*RYGTRRPTGLAMSEM*CLRCPAFGTAQ*PAKSRRRMS
                     LPTGQWSACRPSISPSYLKLDRLILDKKKIAWPRAQISWKNLSIT*KARSPR*SAN"
     misc_feature    join(2289..2298,1..605)
                     /label=rep (pMB1)
ORIGIN
        1 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
       61 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      781 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atcactctgt ggtctcacag
      841 ataagtttgt aaccatgggt tgaaccatgg gttgaaccat gggttgaggt tgagaccacg
      901 aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
      961 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1021 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1081 tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa ctggtccaga
     1141 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1201 tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     1261 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     1321 aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag
     1381 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     1441 gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg gttacggtga
     1501 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     1561 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     1621 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     1681 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     1741 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     1801 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     1861 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     1921 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     1981 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     2041 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccat tacgtaaaag
     2101 gcgagatcac caaggtagtc ggcaaataac tgtcagacca agtttactca tatatacttt
     2161 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     2221 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     2281 aaaagatcaa aggatctt
//
