LOCUS       Exported                2979 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local.rgkiwi.3o-7ouehue
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 96 documents from 
            Done.gb [pL0-G] EYFP(from pL0-G_EYFP).
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      589
                     /label=ORI
     primer_bind     689..708
                     /label=G00101
     misc_feature    complement(828..846)
                     /label=RJ_00079Ra
     misc_feature    830..835
                     /label=BsaI
     protein_bind    830..835
                     /label=BsaI Bind
     misc_feature    complement(831..849)
                     /label=RJ_00116Ra
     misc_feature    837..840
                     /label=pL0 5-G
                     /label=nonstandard type:  gg_overhang
     misc_feature    837..840
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    841..846
                     /label=Kozak
                     /label=nonstandard type:  motif
     misc_feature    845..850
                     /label=NcoI
     CDS             847..1566
                     /label=EYFP
     primer_bind     847..864
                     /label=RJ_00079Fc
     misc_feature    850..1563
                     /label=EYFP
                     /label=nonstandard type:  domain
     primer_bind     850..867
                     /label=RJ_00116Fa
     misc_feature    complement(1544..1563)
                     /label=RJ_00081Rc
     misc_feature    complement(1544..1563)
                     /label=RJ_00082Rc
     primer_bind     1564..1584
                     /label=RJ_00081Fa
     primer_bind     1564..1584
                     /label=RJ_00082Fa
     misc_feature    1567..1570
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    1567..1570
                     /label=pL0 G-3
                     /label=nonstandard type:  gg_overhang
     misc_feature    complement(1572..1577)
                     /label=BsaI
     protein_bind    complement(1572..1577)
                     /label=BsaI Bind
     misc_feature    1727..1750
                     /label=Pamp
     misc_feature    complement(1742..1762)
                     /label=JH856R
     misc_feature    1797..1955
                     /label=Spec
     rep_origin      complement(join(2904..2979,1..607))
                     /direction=LEFT
                     /label=ColE1 origin
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaaggt
      841 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      901 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      961 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1021 cccaccctcg tgaccacctt cggctacggc ctgcagtgct tcgcccgcta ccccgaccac
     1081 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1141 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1201 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1261 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     1321 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     1381 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1441 aaccactacc tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     1501 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1561 aagtgagctt tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc
     1621 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     1681 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1741 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc
     1801 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     1861 ttttcatggc ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa
     1921 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     1981 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
     2041 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     2101 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat
     2161 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     2221 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     2281 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     2341 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     2401 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     2461 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     2521 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     2581 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     2641 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     2701 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     2761 atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca
     2821 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     2881 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2941 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
