LOCUS       Exported                3009 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  [pL0-G] Gal4-NLS-HA-VP16.
ACCESSION   urn.local.jones.uly-8qj5t57
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 2 documents from 
            Activators.gb
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(17..38)
                     /label=pHAGE-A7050-6550
                     /note="Geneious type: Promoter_prokaryotic"
                     /note="/ApEinfo_fwdcolor=#85dae9"
                     /note="/ApEinfo_revcolor=#85dae9"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pTK143 1732\-\pAAVio-CA; 
                     pTW073(pTK143 no IRES GFP)"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/vntifkey=28"
     primer_bind     222..241
                     /label=RepO-R
                     /note="/ApEinfo_fwdcolor=#84b0dc"
                     /note="/ApEinfo_revcolor=#84b0dc"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pTW037 DI pDEST"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    261..285
                     /label=pHAGE-S7501-8000
                     /note="Geneious type: Promoter_prokaryotic"
                     /note="/ApEinfo_fwdcolor=#d6b295"
                     /note="/ApEinfo_revcolor=#d6b295"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pTK143 1732\-\pAAVio-CA; 
                     pTW073(pTK143 no IRES GFP)"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/vntifkey=28"
     misc_feature    complement(528..549)
                     /label=pHAGE-A7550-7050
                     /note="Geneious type: Promoter_prokaryotic"
                     /note="/ApEinfo_fwdcolor=#75c6a9"
                     /note="/ApEinfo_revcolor=#75c6a9"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pTK143 1732\-\pAAVio-CA; 
                     pTW073(pTK143 no IRES GFP)"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/vntifkey=28"
     primer_bind     530..550
                     /label=TW_HDV-seq-R
                     /note="/ApEinfo_fwdcolor=#75c6a9"
                     /note="/ApEinfo_revcolor=#75c6a9"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pTW031 short HDV Active (-); 
                     pTW037DI pDEST; pTW039 Genomic HDV Active (-); pTW040 
                     GenomicHDV Inactive (-)"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    complement(537..548)
                     /label=DrdI, AasI, DseDI
                     /note="Geneious type: restriction site"
                     /note="/ApEinfo_fwdcolor=#9eafd2"
                     /note="/ApEinfo_revcolor=#9eafd2"
                     /note="/Recognition_pattern=GACNNNN^NNGTC"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pTW031 short HDV Active (-); 
                     pTW039Genomic HDV Active (-); pTW040 Genomic HDV Inactive 
                     (-)"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/annotation_group=GACNNNNNNGTC"
     misc_feature    complement(557..568)
                     /label=BdaI
                     /note="Geneious type: restriction site"
                     /note="/ApEinfo_fwdcolor=#ffef86"
                     /note="/ApEinfo_revcolor=#ffef86"
                     /note="/Recognition_pattern=(10/12)TGANNNNNNTCA(12/10)"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pTW031 short HDV Active (-); 
                     pTW039Genomic HDV Active (-); pTW040 Genomic HDV Inactive 
                     (-)"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/annotation_group=TGANNNNNNTCA"
     rep_origin      589
                     /label=ORI
                     /note="/ApEinfo_fwdcolor=#faac61"
                     /note="/ApEinfo_revcolor=#faac61"
     primer_bind     660..680
                     /label=TW_lvl0_seq_Rev1
                     /note="/ApEinfo_fwdcolor=#9eafd2"
                     /note="/ApEinfo_revcolor=#9eafd2"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     689..708
                     /label=G00101
     primer_bind     702..721
                     /label=JG_1.80
     misc_feature    763..777
                     /label=15 bp SapI Overhang
                     /note="/ApEinfo_fwdcolor=#84b0dc"
                     /note="/ApEinfo_revcolor=#84b0dc"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     824..842
                     /label=RJ_00029Fa
     misc_feature    830..835
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/ApEinfo_fwdcolor=#ffef86"
                     /note="/ApEinfo_revcolor=#ffef86"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
                     /note="/annotation_group=GGTCTC"
     protein_bind    830..835
                     /label=BsaI Bind
                     /note="/ApEinfo_fwdcolor=#f58a5e"
                     /note="/ApEinfo_revcolor=#f58a5e"
                     /note="/created_by=User"
     misc_feature    837..840
                     /label=pL0 5-G
                     /label=nonstandard type:  Gg_overhang
                     /note="/ApEinfo_fwdcolor=#d6b295"
                     /note="/ApEinfo_revcolor=#d6b295"
                     /note="/created_by=User"
     misc_feature    841..846
                     /label=Kozak
                     /label=nonstandard type:  motif
                     /note="/ApEinfo_fwdcolor=#f58a5e"
                     /note="/ApEinfo_revcolor=#f58a5e"
                     /note="/created_by=User"
     CDS             847..1596
                     /codon_start=1
                     /label=Gal4-NLS-HA-VP16
                     /note="/ApEinfo_fwdcolor=#84b0dc"
                     /note="/ApEinfo_revcolor=#84b0dc"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSMGPKKKRKVGSGYPYD
                     VPDYAGSGAPPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSA
                     PYGALDMADFEFEQMFTDALGIDEYGG"
     misc_feature    850..1287
                     /label=Gal4 DBD
                     /label=nonstandard type:  domain
                     /note="/ApEinfo_fwdcolor=#c7b0e3"
                     /note="/ApEinfo_revcolor=#c7b0e3"
                     /note="/created_by=User"
     misc_feature    877..960
                     /label=Zn(2)-C6fungal-type.{ECO:0000255|PROSITE-ProRul
                     /label=Zn(2)-C6fungal-type.{ECO:0000255|PROSITE-ProRule:PRU
                     00227}.
                     /note="Geneious type: dna_binding_domain"
                     /note="/ApEinfo_fwdcolor=#faac61"
                     /note="/ApEinfo_revcolor=#faac61"
                     /note="/Transferred_From=Gal4 DBD (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     protein_bind    994..1125
                     /label=Leucine Zipper
                     /note="/ApEinfo_fwdcolor=#85dae9"
                     /note="/ApEinfo_revcolor=#85dae9"
                     /note="/created_by=jones"
     misc_feature    1294..1314
                     /label=NLS
                     /label=nonstandard type:  motif
                     /note="/ApEinfo_fwdcolor=#ff9ccd"
                     /note="/ApEinfo_revcolor=#ff9ccd"
                     /note="/Transferred_From=Digestion of pRJ_00009 PCR Product
                     -Fragment 2, Digestion of pRJ_00010 PCR Product - 
                     Fragment2, Digestion of pRJ_00011 PCR Product - Fragment 
                     2,Digestion of pRJ_00012 PCR Product - Fragment 2, 
                     Digestionof pRJ_00013 PCR Product - Fragment 2, 
                     pRJ_00009,pRJ_00009 PCR Product, pRJ_00010, pRJ_00010 PCR 
                     Product,pRJ_00011, pRJ_00011 PCR Product, pRJ_00012, 
                     pRJ_00012 PCRProduct, pRJ_00013, pRJ_00013 PCR Product, 
                     pRJ_00095,pRJ_00096, pRJ_00097, pRJ_00098, pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     1294..1314
                     /label=RJ_00068Fa
     misc_feature    1315..1323
                     /label=GSG
                     /label=nonstandard type:  motif
                     /note="/ApEinfo_fwdcolor=#84b0dc"
                     /note="/ApEinfo_revcolor=#84b0dc"
                     /note="/created_by=User"
     sig_peptide     1324..1350
                     /label=HA-tag
                     /note="/ApEinfo_fwdcolor=#f58a5e"
                     /note="/ApEinfo_revcolor=#f58a5e"
                     /note="/Transferred_From=(4) Digestion of pRJ_00095 - 
                     Fragment1, (4) Digestion of pRJ_00096 - Fragment 1, (4) 
                     Digestionof pRJ_00097 - Fragment 1, (4) Digestion of 
                     pRJ_00098 -Fragment 1, (4) Digestion of pRJ_00099 - 
                     Fragment 1,Digestion of pRJ_00095 - Fragment 1, Digestion 
                     ofpRJ_00096 - Fragment 1, Digestion of pRJ_00097 - 
                     Fragment1, Digestion of pRJ_00098 - Fragment 1, Digestion 
                     ofpRJ_00099 - Fragment 1, pRJ_00095, pRJ_00096, 
                     pRJ_00097,pRJ_00098, pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     complement(1336..1354)
                     /label=RJ_00495Ra
     misc_feature    1336..1350
                     /label=Infusion
                     /label=nonstandard type:  Gibson_overhang
                     /note="/ApEinfo_fwdcolor=#d6b295"
                     /note="/ApEinfo_revcolor=#d6b295"
                     /note="/created_by=User"
     primer_bind     complement(1339..1359)
                     /label=RJ_00164Ra
     primer_bind     1351..1367
                     /label=RJ_00456Fa
     primer_bind     1351..1366
                     /label=RJ_00495Fb
     misc_feature    1351..1359
                     /label=GSG
                     /label=nonstandard type:  motif
                     /note="/ApEinfo_fwdcolor=#d6b295"
                     /note="/ApEinfo_revcolor=#d6b295"
                     /note="/created_by=User"
     misc_feature    1360..1593
                     /label=VP16
                     /label=nonstandard type:  domain
                     /note="/ApEinfo_fwdcolor=#84b0dc"
                     /note="/ApEinfo_revcolor=#84b0dc"
                     /note="/Transferred_From=Digestion of pRJ_00009 PCR Product
                     -Fragment 2, pRJ_00009, pRJ_00009 PCR Product, pRJ_00095"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    1432..1464
                     /label=VP16min
                     /label=nonstandard type:  domain
                     /note="/ApEinfo_fwdcolor=#d6b295"
                     /note="/ApEinfo_revcolor=#d6b295"
                     /note="/Transferred_From=VP16 (pBjh_4066)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    1552..1578
                     /label=9aaTAD
                     /label=nonstandard type:  motif
                     /note="/ApEinfo_fwdcolor=#c6c9d1"
                     /note="/ApEinfo_revcolor=#c6c9d1"
                     /note="/created_by=User"
     primer_bind     complement(1573..1593)
                     /label=RJ_00495Rb
     primer_bind     complement(1574..1593)
                     /label=RJ_00050Ra
     primer_bind     complement(1587..1606)
                     /label=RJ_00495Rc
     primer_bind     1596..1616
                     /label=RJ_00495Fa
     primer_bind     1597..1617
                     /label=RJ_00164Fa
     misc_feature    1597..1600
                     /label=pL0 G-3
                     /label=nonstandard type:  Gg_overhang
                     /note="/ApEinfo_fwdcolor=#f8d3a9"
                     /note="/ApEinfo_revcolor=#f8d3a9"
                     /note="/created_by=User"
     protein_bind    complement(1602..1607)
                     /label=BsaI Bind
                     /note="/ApEinfo_fwdcolor=#d6b295"
                     /note="/ApEinfo_revcolor=#d6b295"
                     /note="/created_by=User"
     misc_feature    complement(1602..1607)
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/ApEinfo_fwdcolor=#ff9ccd"
                     /note="/ApEinfo_revcolor=#ff9ccd"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
                     /note="/annotation_group=GGTCTC"
     primer_bind     complement(1704..1722)
                     /label=JG_1.81
     misc_feature    1757..1780
                     /label=Pamp
                     /note="Geneious type: Promoter_prokaryotic"
                     /note="/ApEinfo_fwdcolor=#c6c9d1"
                     /note="/ApEinfo_revcolor=#c6c9d1"
                     /note="/created_by=User"
     primer_bind     complement(1772..1792)
                     /label=JH856R
     misc_feature    1827..2839
                     /label=spec
                     /note="/ApEinfo_fwdcolor=#9eafd2"
                     /note="/ApEinfo_revcolor=#9eafd2"
     primer_bind     complement(2300..2323)
                     /label=RJ_00189Ra
     primer_bind     2309..2329
                     /label=RJ_00190Fa
     rep_origin      complement(join(2934..3009,1..607))
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="/ApEinfo_fwdcolor=#d6b295"
                     /note="/ApEinfo_revcolor=#d6b295"
                     /note="/created_by=User"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaaggt
      841 gccaccatga agctactgtc ctctatcgaa caagcatgcg atatttgccg acttaaaaag
      901 ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt
      961 cgctactctc ccaaaaccaa aaggtccccg ctgactaggg cacatctgac agaagtggaa
     1021 tcaaggctag aaagactgga acagctattt ctactgattt ttcctcgaga ggaccttgac
     1081 atgattttga aaatggattc tttacaggat ataaaagcat tgttaacagg attatttgta
     1141 caagataatg tgaataaaga tgccgtcaca gatagattgg cttcagtgga gactgatatg
     1201 cctctaacat tgagacagca tagaataagt gcgacatcat catcggaaga gagtagtaac
     1261 aaaggtcaaa gacagttgac tgtatcgatg ggccccaaga agaagaggaa ggtgggatct
     1321 ggctacccat acgacgtgcc agattacgcc ggatcaggtg cccccccgac cgatgtcagc
     1381 ctgggggacg agctccactt agacggcgag gacgtggcga tggcgcatgc cgacgcgcta
     1441 gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg atttaccccc
     1501 cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga gcagatgttt
     1561 accgatgccc ttggaattga cgagtacggt gggtgagctt tgagaccacg aagtggctct
     1621 tcagtggacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     1681 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1741 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1801 ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa ctggtccaga accttgaccg
     1861 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttt
     1921 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     1981 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     2041 aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     2101 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     2161 ggatggcggc ctgaagccac acagcgatat tgatttgctg gttacggtga ccgtaaggct
     2221 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     2281 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     2341 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     2401 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     2461 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     2521 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     2581 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     2641 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     2701 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     2761 cttggcctcg cgcgcagatc agttggaaga atttgtccat tacgtaaaag gcgagatcac
     2821 caaggtagtc ggcaaataac tgtcagacca agtttactca tatatacttt agattgattt
     2881 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2941 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3001 aggatcttc
//
