LOCUS       Exported                4434 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  [pL0-G] Gal4-NLS-HA-VPR.
ACCESSION   urn.local.jones.umb-8qj5t5b
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: pRJ_00137.gb
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(17..38)
                     /label=pHAGE-A7050-6550
                     /note="Geneious type: Promoter_prokaryotic"
                     /note="/ApEinfo_fwdcolor=#d6b295"
                     /note="/ApEinfo_revcolor=#d6b295"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pTK143 1732\-\pAAVio-CA; 
                     pTW073(pTK143 no IRES GFP)"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/vntifkey=28"
     primer_bind     222..241
                     /label=RepO-R
                     /note="/ApEinfo_fwdcolor=#84b0dc"
                     /note="/ApEinfo_revcolor=#84b0dc"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pTW037 DI pDEST"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    261..285
                     /label=pHAGE-S7501-8000
                     /note="Geneious type: Promoter_prokaryotic"
                     /note="/ApEinfo_fwdcolor=#d6b295"
                     /note="/ApEinfo_revcolor=#d6b295"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pTK143 1732\-\pAAVio-CA; 
                     pTW073(pTK143 no IRES GFP)"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/vntifkey=28"
     misc_feature    complement(528..549)
                     /label=pHAGE-A7550-7050
                     /note="Geneious type: Promoter_prokaryotic"
                     /note="/ApEinfo_fwdcolor=#b4abac"
                     /note="/ApEinfo_revcolor=#b4abac"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pTK143 1732\-\pAAVio-CA; 
                     pTW073(pTK143 no IRES GFP)"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/vntifkey=28"
     primer_bind     530..550
                     /label=TW_HDV-seq-R
                     /note="/ApEinfo_fwdcolor=#c6c9d1"
                     /note="/ApEinfo_revcolor=#c6c9d1"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pTW031 short HDV Active (-); 
                     pTW037DI pDEST; pTW039 Genomic HDV Active (-); pTW040 
                     GenomicHDV Inactive (-)"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    complement(537..548)
                     /label=DrdI, AasI, DseDI
                     /note="Geneious type: restriction site"
                     /note="/ApEinfo_fwdcolor=#b4abac"
                     /note="/ApEinfo_revcolor=#b4abac"
                     /note="/Recognition_pattern=GACNNNN^NNGTC"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pTW031 short HDV Active (-); 
                     pTW039Genomic HDV Active (-); pTW040 Genomic HDV Inactive 
                     (-)"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/annotation_group=GACNNNNNNGTC"
     misc_feature    complement(557..568)
                     /label=BdaI
                     /note="Geneious type: restriction site"
                     /note="/ApEinfo_fwdcolor=#faac61"
                     /note="/ApEinfo_revcolor=#faac61"
                     /note="/Recognition_pattern=(10/12)TGANNNNNNTCA(12/10)"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pTW031 short HDV Active (-); 
                     pTW039Genomic HDV Active (-); pTW040 Genomic HDV Inactive 
                     (-)"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/annotation_group=TGANNNNNNTCA"
     rep_origin      589
                     /label=ORI
                     /note="/ApEinfo_fwdcolor=#c6c9d1"
                     /note="/ApEinfo_revcolor=#c6c9d1"
     primer_bind     660..680
                     /label=TW_lvl0_seq_Rev1
                     /note="/ApEinfo_fwdcolor=#c6c9d1"
                     /note="/ApEinfo_revcolor=#c6c9d1"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     689..708
                     /label=G00101
     misc_feature    763..777
                     /label=15 bp SapI Overhang
                     /note="/ApEinfo_fwdcolor=#b1ff67"
                     /note="/ApEinfo_revcolor=#b1ff67"
                     /note="/Transferred_From=pJB770"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     824..842
                     /label=RJ_00029Fa
     misc_feature    830..835
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/ApEinfo_fwdcolor=#75c6a9"
                     /note="/ApEinfo_revcolor=#75c6a9"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
                     /note="/annotation_group=GGTCTC"
     protein_bind    830..835
                     /label=BsaI Bind
                     /note="/ApEinfo_fwdcolor=#c7b0e3"
                     /note="/ApEinfo_revcolor=#c7b0e3"
                     /note="/created_by=User"
     misc_feature    837..840
                     /label=pL0 5-G
                     /label=nonstandard type:  Gg_overhang
                     /note="/ApEinfo_fwdcolor=#85dae9"
                     /note="/ApEinfo_revcolor=#85dae9"
                     /note="/created_by=User"
     misc_feature    841..846
                     /label=Kozak
                     /label=nonstandard type:  motif
                     /note="/ApEinfo_fwdcolor=#ff9ccd"
                     /note="/ApEinfo_revcolor=#ff9ccd"
                     /note="/created_by=User"
     CDS             847..3021
                     /codon_start=1
                     /label=Gal4-NLS-HA-VPR
                     /note="/ApEinfo_fwdcolor=#84b0dc"
                     /note="/ApEinfo_revcolor=#84b0dc"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSMGPKKKRKVGSGYPYD
                     VPDYAGSGSPGIRRLDALISTSLYKKAGYKEASGSGRADALDDFDLDMLGSDALDDFDL
                     DMLGSDALDDFDLDMLGSDALDDFDLDMLINSRSSGSPKKKRKVGSQYLPDTDDRHRIE
                     EKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTI
                     NYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGP
                     PQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQ
                     LLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFS
                     SIADMDFSALLGSGSGSRDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSP
                     WANRPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAV
                     KALREMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELN
                     EILDTFLNDECLLHAMHISTGLSIFDTSLF"
     misc_feature    850..1287
                     /label=Gal4 DBD
                     /label=nonstandard type:  domain
                     /note="/ApEinfo_fwdcolor=#84b0dc"
                     /note="/ApEinfo_revcolor=#84b0dc"
                     /note="/created_by=User"
     misc_feature    877..960
                     /label=Zn(2)-C6fungal-type.{ECO:0000255|PROSITE-ProRul
                     /label=Zn(2)-C6fungal-type.{ECO:0000255|PROSITE-ProRule:PRU
                     00227}.
                     /note="Geneious type: dna_binding_domain"
                     /note="/ApEinfo_fwdcolor=#75c6a9"
                     /note="/ApEinfo_revcolor=#75c6a9"
                     /note="/Transferred_From=Gal4 DBD (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     protein_bind    994..1125
                     /label=Leucine Zipper
                     /note="/ApEinfo_fwdcolor=#f8d3a9"
                     /note="/ApEinfo_revcolor=#f8d3a9"
                     /note="/created_by=jones"
     misc_feature    1294..1314
                     /label=NLS
                     /label=nonstandard type:  motif
                     /note="/ApEinfo_fwdcolor=#d6b295"
                     /note="/ApEinfo_revcolor=#d6b295"
                     /note="/Transferred_From=Digestion of pRJ_00009 PCR Product
                     -Fragment 2, Digestion of pRJ_00010 PCR Product - 
                     Fragment2, Digestion of pRJ_00011 PCR Product - Fragment 
                     2,Digestion of pRJ_00012 PCR Product - Fragment 2, 
                     Digestionof pRJ_00013 PCR Product - Fragment 2, 
                     pRJ_00009,pRJ_00009 PCR Product, pRJ_00010, pRJ_00010 PCR 
                     Product,pRJ_00011, pRJ_00011 PCR Product, pRJ_00012, 
                     pRJ_00012 PCRProduct, pRJ_00013, pRJ_00013 PCR Product, 
                     pRJ_00095,pRJ_00096, pRJ_00097, pRJ_00098, pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     1294..1314
                     /label=RJ_00068Fa
     misc_feature    1315..1323
                     /label=GSG
                     /label=nonstandard type:  motif
                     /note="/ApEinfo_fwdcolor=#75c6a9"
                     /note="/ApEinfo_revcolor=#75c6a9"
                     /note="/created_by=User"
     sig_peptide     1324..1350
                     /label=HA-tag
                     /note="/ApEinfo_fwdcolor=#d6b295"
                     /note="/ApEinfo_revcolor=#d6b295"
                     /note="/Transferred_From=(4) Digestion of pRJ_00095 - 
                     Fragment1, (4) Digestion of pRJ_00096 - Fragment 1, (4) 
                     Digestionof pRJ_00097 - Fragment 1, (4) Digestion of 
                     pRJ_00098 -Fragment 1, (4) Digestion of pRJ_00099 - 
                     Fragment 1,Digestion of pRJ_00095 - Fragment 1, Digestion 
                     ofpRJ_00096 - Fragment 1, Digestion of pRJ_00097 - 
                     Fragment1, Digestion of pRJ_00098 - Fragment 1, Digestion 
                     ofpRJ_00099 - Fragment 1, pRJ_00095, pRJ_00096, 
                     pRJ_00097,pRJ_00098, pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    1336..1350
                     /label=Infusion
                     /label=nonstandard type:  Gibson_overhang
                     /note="/ApEinfo_fwdcolor=#84b0dc"
                     /note="/ApEinfo_revcolor=#84b0dc"
                     /note="/created_by=User"
     misc_feature    1351..1359
                     /label=GSG
                     /label=nonstandard type:  motif
                     /note="/ApEinfo_fwdcolor=#85dae9"
                     /note="/ApEinfo_revcolor=#85dae9"
                     /note="/created_by=User"
     misc_feature    1397..1417
                     /label=attB1
                     /label=nonstandard type:  recomb_site
                     /note="/ApEinfo_fwdcolor=#ffef86"
                     /note="/ApEinfo_revcolor=#ffef86"
     misc_feature    1429..1443
                     /label=Linker
                     /label=nonstandard type:  motif
                     /note="/ApEinfo_fwdcolor=#85dae9"
                     /note="/ApEinfo_revcolor=#85dae9"
                     /note="/created_by=User"
     misc_feature    1444..1611
                     /label=VP64
                     /label=nonstandard type:  domain
                     /note="/ApEinfo_fwdcolor=#c6c9d1"
                     /note="/ApEinfo_revcolor=#c6c9d1"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     -Fragment 2, Digestion of pRJ_00011 PCR Product - 
                     Fragment2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00011, 
                     pRJ_00011PCR Product, pRJ_00096, pRJ_00097"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    1450..1482
                     /label=VP16min
                     /label=nonstandard type:  domain
                     /note="/ApEinfo_fwdcolor=#b7e6d7"
                     /note="/ApEinfo_revcolor=#b7e6d7"
                     /note="/Transferred_From=VP64 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    1489..1521
                     /label=VP16min
                     /label=nonstandard type:  domain
                     /note="/ApEinfo_fwdcolor=#c7b0e3"
                     /note="/ApEinfo_revcolor=#c7b0e3"
                     /note="/Transferred_From=VP64 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    1528..1560
                     /label=VP16min
                     /label=nonstandard type:  domain
                     /note="/ApEinfo_fwdcolor=#ff9ccd"
                     /note="/ApEinfo_revcolor=#ff9ccd"
                     /note="/Transferred_From=VP64 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    1567..1599
                     /label=VP16min
                     /label=nonstandard type:  domain
                     /note="/ApEinfo_fwdcolor=#f58a5e"
                     /note="/ApEinfo_revcolor=#f58a5e"
                     /note="/Transferred_From=VP64 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(1588..1611)
                     /label=RJ_00050Rc
     misc_feature    1612..1623
                     /label=Linker
                     /label=nonstandard type:  motif
                     /note="/ApEinfo_fwdcolor=#d59687"
                     /note="/ApEinfo_revcolor=#d59687"
                     /note="/created_by=User"
     misc_feature    1624..1647
                     /label=SV40
                     /label=nonstandard type:  motif
                     /note="/ApEinfo_fwdcolor=#ffef86"
                     /note="/ApEinfo_revcolor=#ffef86"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     -Fragment 2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00096"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    1648..2430
                     /label=p65
                     /label=nonstandard type:  domain
                     /note="/ApEinfo_fwdcolor=#9eafd2"
                     /note="/ApEinfo_revcolor=#9eafd2"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     -Fragment 2, Digestion of pRJ_00012 PCR Product - 
                     Fragment2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00012, 
                     pRJ_00012PCR Product, pRJ_00096, pRJ_00098"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    1693..1704
                     /label=NLS
                     /label=nonstandard type:  motif
                     /note="/ApEinfo_fwdcolor=#b4abac"
                     /note="/ApEinfo_revcolor=#b4abac"
                     /note="/Transferred_From=p65 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     1711..1734
                     /label=RJ_00203Fa
     primer_bind     1986..2003
                     /label=RJ_00119Fa
     misc_feature    2035..2169
                     /label=Activation domain
                     /note="Geneious type: Region_of_interest"
                     /note="/ApEinfo_fwdcolor=#b4abac"
                     /note="/ApEinfo_revcolor=#b4abac"
                     /note="/Transferred_From=p65 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    2398..2424
                     /label=9aaTAD
                     /label=nonstandard type:  motif
                     /note="/ApEinfo_fwdcolor=#d59687"
                     /note="/ApEinfo_revcolor=#d59687"
                     /note="/Transferred_From=p65 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(2411..2430)
                     /label=RJ_00050Rd
     misc_feature    2431..2448
                     /label=GS-rich Linker
                     /label=nonstandard type:  motif
                     /note="/ApEinfo_fwdcolor=#c7b0e3"
                     /note="/ApEinfo_revcolor=#c7b0e3"
                     /note="/created_by=User"
     misc_feature    2449..3018
                     /label=Rta
                     /label=nonstandard type:  domain
                     /note="/ApEinfo_fwdcolor=#84b0dc"
                     /note="/ApEinfo_revcolor=#84b0dc"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     -Fragment 2, Digestion of pRJ_00013 PCR Product - 
                     Fragment2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00013, 
                     pRJ_00013PCR Product, pRJ_00096, pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    2536..2700
                     /label=Pro-rich
                     /note="Geneious type: Compositionally_biased"
                     /note="/ApEinfo_fwdcolor=#faac61"
                     /note="/ApEinfo_revcolor=#faac61"
                     /note="/Transferred_From=Rta (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(2996..3018)
                     /label=RJ_00050Rb
     misc_feature    3022..3025
                     /label=pL0 G-3
                     /label=nonstandard type:  Gg_overhang
                     /note="/ApEinfo_fwdcolor=#84b0dc"
                     /note="/ApEinfo_revcolor=#84b0dc"
                     /note="/created_by=User"
     protein_bind    complement(3027..3032)
                     /label=BsaI Bind
                     /note="/ApEinfo_fwdcolor=#ffef86"
                     /note="/ApEinfo_revcolor=#ffef86"
                     /note="/created_by=User"
     misc_feature    complement(3027..3032)
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/ApEinfo_fwdcolor=#b4abac"
                     /note="/ApEinfo_revcolor=#b4abac"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
                     /note="/annotation_group=GGTCTC"
     misc_feature    3182..3205
                     /label=Pamp
                     /note="Geneious type: Promoter_prokaryotic"
                     /note="/ApEinfo_fwdcolor=#b1ff67"
                     /note="/ApEinfo_revcolor=#b1ff67"
                     /note="/created_by=User"
     primer_bind     complement(3197..3217)
                     /label=JH856R
     misc_feature    3252..4264
                     /label=Spec
                     /note="/ApEinfo_fwdcolor=#75c6a9"
                     /note="/ApEinfo_revcolor=#75c6a9"
     primer_bind     complement(3725..3748)
                     /label=RJ_00189Ra
     primer_bind     3734..3754
                     /label=RJ_00190Fa
     rep_origin      complement(join(4359..4434,1..607))
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="/ApEinfo_fwdcolor=#d59687"
                     /note="/ApEinfo_revcolor=#d59687"
                     /note="/created_by=User"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaaggt
      841 gccaccatga agctactgtc ctctatcgaa caagcatgcg atatttgccg acttaaaaag
      901 ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt
      961 cgctactctc ccaaaaccaa aaggtccccg ctgactaggg cacatctgac agaagtggaa
     1021 tcaaggctag aaagactgga acagctattt ctactgattt ttcctcgaga ggaccttgac
     1081 atgattttga aaatggattc tttacaggat ataaaagcat tgttaacagg attatttgta
     1141 caagataatg tgaataaaga tgccgtcaca gatagattgg cttcagtgga gactgatatg
     1201 cctctaacat tgagacagca tagaataagt gcgacatcat catcggaaga gagtagtaac
     1261 aaaggtcaaa gacagttgac tgtatcgatg ggccccaaga agaagaggaa ggtgggatct
     1321 ggctacccat acgacgtgcc agattacgcc ggatcaggtt cgccagggat ccgtcgactt
     1381 gacgcgttga tatcaacaag tttgtacaaa aaagcaggct acaaagaggc cagcggttcc
     1441 ggacgggctg acgcattgga cgattttgat ctggatatgc tgggaagtga cgccctcgat
     1501 gattttgacc ttgacatgct tggttcggat gcccttgatg actttgacct cgacatgctc
     1561 ggcagtgacg cccttgatga tttcgacctg gacatgctga ttaactctag aagttccgga
     1621 tctccgaaaa agaaacgcaa agttggtagc cagtacctgc ccgacaccga cgaccggcac
     1681 cggatcgagg aaaagcggaa gcggacctac gagacattca agagcatcat gaagaagtcc
     1741 cccttcagcg gccccaccga ccctagacct ccacctagaa gaatcgccgt gcccagcaga
     1801 tccagcgcca gcgtgccaaa acctgccccc cagccttacc ccttcaccag cagcctgagc
     1861 accatcaact acgacgagtt ccctaccatg gtgttcccca gcggccagat ctctcaggcc
     1921 tctgctctgg ctccagcccc tcctcaggtg ctgcctcagg ctcctgctcc tgcaccagct
     1981 ccagccatgg tgtctgcact ggctcaggca ccagcacccg tgcctgtgct ggctcctgga
     2041 cctccacagg ctgtggctcc accagcccct aaacctacac aggccggcga gggcacactg
     2101 tctgaagctc tgctgcagct gcagttcgac gacgaggatc tgggagccct gctgggaaac
     2161 agcaccgatc ctgccgtgtt caccgacctg gccagcgtgg acaacagcga gttccagcag
     2221 ctgctgaacc agggcatccc tgtggcccct cacaccaccg agcccatgct gatggaatac
     2281 cccgaggcca tcacccggct cgtgacaggc gctcagaggc ctcctgatcc agctcctgcc
     2341 cctctgggag caccaggcct gcctaatgga ctgctgtctg gcgacgagga cttcagctct
     2401 atcgccgata tggatttctc agccttgctg ggctctggca gcggcagccg ggattccagg
     2461 gaagggatgt ttttgccgaa gcctgaggcc ggctccgcta ttagtgacgt gtttgagggc
     2521 cgcgaggtgt gccagccaaa acgaatccgg ccatttcatc ctccaggaag tccatgggcc
     2581 aaccgcccac tccccgccag cctcgcacca acaccaaccg gtccagtaca tgagccagtc
     2641 gggtcactga ccccggcacc agtccctcag ccactggatc cagcgcccgc agtgactccc
     2701 gaggccagtc acctgttgga ggatcccgat gaagagacga gccaggctgt caaagccctt
     2761 cgggagatgg ccgatactgt gattccccag aaggaagagg ctgcaatctg tggccaaatg
     2821 gacctttccc atccgccccc aaggggccat ctggatgagc tgacaaccac acttgagtcc
     2881 atgaccgagg atctgaacct ggactcaccc ctgaccccgg aattgaacga gattctggat
     2941 accttcctga acgacgagtg cctcttgcat gccatgcata tcagcacagg actgtccatc
     3001 ttcgacacat ctctgttttg agctttgaga ccacgaagtg gctcttcagt ggacgaaagg
     3061 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     3121 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3181 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3241 aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     3301 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttttgggg tacagtctat
     3361 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     3421 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga
     3481 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     3541 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     3601 gccacacagc gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     3661 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     3721 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     3781 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     3841 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     3901 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     3961 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     4021 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     4081 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     4141 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     4201 agatcagttg gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa
     4261 ataactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4321 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4381 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
