LOCUS       Exported                2871 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: benchling_export 
            (7).gb [pL0-G] BM3R1-NLS
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     689..708
                     /label=G00101
                     /note="Mismatches: 0 created by: primer3 annotation group: 
                     240459434107348958d42c-86c4-4f53-8a37-ebf73f5de331 %GC: 
                     50.0 Tm: 58.3 Hairpin Tm: None Self Dimer Tm: None 
                     Sequence: ATTACCGCCTTTGAGTGAGC"
     protein_bind    830..835
                     /label=BsaI Bind
                     /note="created by: User"
     misc_feature    complement(832..849)
                     /label=RJ_00053Ra
                     /label=nonstandard type:  primer_bind_...
                     /note="Sequence: GGTGGCGGCACCTTGAGA Hairpin Tm: 41.8 Tm: 
                     62.3 Self Dimer Tm: 44.0 %GC: 66.7 created by: primer3"
     misc_feature    837..840
                     /label=pL0 5-G
                     /label=nonstandard type:  gg_overhang
                     /note="created by: User"
     misc_feature    841..849
                     /label=Kozak
                     /label=nonstandard type:  motif
                     /note="created by: User"
     CDS             850..1458
                     /codon_start=1
                     /label=BM3R1-NLS
                     /note="ApEinfo_fwdcolor: #55ff5f ApEinfo_revcolor: green 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 NCBI Feature Key: CDS modified by: User"
                     /translation="MESTPTKQKAIFSASLLLFAERGFDATTMPMIAENAKVGAGTIYR
                     YFKNKESLVNELFQQHVNEFLQCIESGLANERDGYRDGFHHIFEGMVTFTKNHPRALGF
                     IKTHSQGTFLTEESRLAYQKLVEFVCTFFREGQKQGVIRNLPENALIAILFGSFMEVYE
                     MIENDYLSLTDELLTGVEESLWAALSRQSASPPKKKRKV"
     primer_bind     850..869
                     /label=RJ_00053Fa
                     /note="Sequence: ATGGAAAGCACCCCCACCAA Hairpin Tm: 32.1 Tm: 
                     62.0 Self Dimer Tm: None %GC: 55.0 created by: primer3"
     misc_feature    854..1425
                     /label=BM3R1
                     /label=nonstandard type:  domain
                     /note="created by: User"
     misc_feature    862..1044
                     /label=HTH (TetR type)
                     /label=nonstandard type:  domain
                     /note="created by: User"
     misc_feature    931..990
                     /label=HTH Motif
                     /label=nonstandard type:  dna_binding_...
                     /note="created by: User"
     misc_feature    1432..1455
                     /label=SV40 NLS
                     /label=nonstandard type:  motif
                     /note="ApEinfo_fwdcolor: #ff3f3c ApEinfo_revcolor: #ff3f3c 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 NCBI Feature Key: misc_feature modified 
                     by: User"
     misc_feature    1459..1462
                     /label=pL0 G-3
                     /label=nonstandard type:  gg_overhang
                     /note="created by: User"
     protein_bind    complement(1464..1469)
                     /label=BsaI Bind
                     /note="created by: User"
     misc_feature    1619..1642
                     /label=Pamp
                     /label=nonstandard type:  promoter_pro...
                     /note="label: AmpR_promoter NCBI Feature Key: promoter 
                     Transferred From: pAT61 Transferred Similarity: 100.00% 
                     modified by: User"
     misc_feature    complement(1634..1654)
                     /label=JH856R
                     /label=nonstandard type:  primer_bind_...
                     /note="Mismatches: 0 created by: primer3 annotation group: 
                     240472336635245b51deb9-23bc-4486-81f1-1293de2ed810 %GC: 
                     47.6 Tm: 56.4 Hairpin Tm: None Self Dimer Tm: 13.2 
                     Sequence: CAGGGTTATTGTCTCATGAGC"
     misc_feature    1688..2701
                     /label=Spec
     rep_origin      complement(join(2796..2871,1..607))
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ApEinfo_fwdcolor: gray50 ApEinfo_revcolor: gray50 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 NCBI Feature Key: rep_origin"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaaggt
      841 gccgccacca tggaaagcac ccccaccaag cagaaggcca tcttcagcgc cagcctgctg
      901 ctgttcgccg agagaggctt tgacgccacc accatgccca tgattgccga gaacgccaaa
      961 gtgggagccg gcaccatcta ccggtacttc aagaacaaag agagcctggt caacgagctg
     1021 ttccagcagc acgtgaacga gtttctgcag tgcatcgaga gcggcctggc caatgagagg
     1081 gacggctaca gagatggctt ccaccacatc ttcgagggca tggtcacctt caccaagaac
     1141 caccccagag ccctgggctt catcaagacc cacagccagg gcacctttct gaccgaggaa
     1201 agccggctgg cctaccagaa actggtggaa ttcgtgtgca ccttcttcag agagggccag
     1261 aaacagggcg tgatccggaa cctgcccgag aatgccctga tcgccatcct gttcggcagc
     1321 ttcatggaag tgtacgagat gatcgagaac gactacctga gcctgaccga cgagctgctg
     1381 accggcgtgg aagaatctct gtgggccgct ctgagcagac agagcgccag cccccccaag
     1441 aaaaagcgga aagtgtgagc tttgagacca cgaagtggct cttcagtgga cgaaagggcc
     1501 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     1561 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1621 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1681 ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     1741 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc
     1801 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     1861 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     1921 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     1981 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2041 acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2101 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2161 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2221 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     2281 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2341 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2401 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2461 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     2521 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     2581 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     2641 tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata
     2701 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2761 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2821 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
