LOCUS       Exported                2369 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local.katherine.m-86yp0zy
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 63 documents from 
            Done.gb 3 4xTFF4 (from pRJ_00065).
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      589
                     /label=ORI
     primer_bind     689..708
                     /label=G00101
     misc_feature    830..835
                     /label=BsaI
     protein_bind    830..835
                     /label=BsaI Bind
     misc_feature    837..840
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    837..840
                     /label=pL0 G-3
                     /label=nonstandard type:  gg_overhang
     3'UTR           841..956
                     /label=4xTFF4
     misc_feature    857..878
                     /label=TFF4
                     /label=nonstandard type:  miR_target_site
     misc_feature    879..900
                     /label=TFF4
                     /label=nonstandard type:  miR_target_site
     misc_feature    901..922
                     /label=TFF4
                     /label=nonstandard type:  miR_target_site
     misc_feature    923..944
                     /label=TFF4
                     /label=nonstandard type:  miR_target_site
     misc_feature    complement(927..956)
                     /label=RJ_00027Ra
     misc_feature    957..960
                     /label=pL0 3-T
                     /label=nonstandard type:  gg_overhang
     misc_feature    957..960
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    complement(962..967)
                     /label=BsaI
     protein_bind    complement(962..967)
                     /label=BsaI Bind
     misc_feature    1117..1140
                     /label=Pamp
     misc_feature    complement(1132..1152)
                     /label=JH856R
     CDS             1187..2199
                     /label=Sm/Sp no DraIII
     rep_origin      complement(join(2294..2369,1..607))
                     /direction=LEFT
                     /label=ColE1 origin
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcagctt
      841 gcggccgcaa atcaacccgc ttgaagtctt taattaaacc gcttgaagtc tttaattaaa
      901 ccgcttgaag tctttaatta aaccgcttga agtctttaat taaactaaag cttgagcaac
      961 tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata
     1021 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     1081 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1141 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa
     1201 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     1261 ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     1321 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     1381 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
     1441 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     1501 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg
     1561 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     1621 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     1681 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     1741 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     1801 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     1861 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     1921 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     1981 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     2041 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     2101 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccat
     2161 tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca agtttactca
     2221 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     2281 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     2341 gaccccgtag aaaagatcaa aggatcttc
//
