LOCUS       Exported                2273 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  [pL0-T] Inert.
ACCESSION   urn.local.jones.tka-8qj5swz
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: pRJ_00094.gb
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      589
                     /label=ORI
     primer_bind     689..708
                     /label=G00101
     protein_bind    830..835
                     /label=BsaI Bind
                     /note="/created_by=User"
     misc_feature    830..835
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
                     /note="/annotation_group=GGTCTC"
     misc_feature    837..840
                     /label=pL0 3-T
                     /label=nonstandard type:  Gg_overhang
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    841..860
                     /label=Inert
                     /note="/created_by=User"
                     /note="/modified_by=User"
     terminator      841..860
                     /label=Inert
                     /note="/created_by=User"
     misc_feature    861..864
                     /label=pL0 T
                     /label=nonstandard type:  Gg_overhang
                     /note="/created_by=User"
                     /note="/modified_by=User"
     protein_bind    complement(866..871)
                     /label=BsaI Bind
                     /note="/created_by=User"
     misc_feature    complement(866..871)
                     /label=BsaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGTCTC(1/5)"
                     /note="/annotation_group=GGTCTC"
     misc_feature    1021..1044
                     /label=Pamp
                     /note="Geneious type: Promoter_prokaryotic"
                     /note="/created_by=User"
     primer_bind     complement(1036..1056)
                     /label=JH856R
     misc_feature    1091..2103
                     /label=Spec
     rep_origin      complement(join(2198..2273,1..607))
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="/created_by=User"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcacaac
      841 ccactggatt gtacaattac cgcttgagac cacgaagtgg ctcttcagtg gacgaaaggg
      901 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      961 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1021 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1081 aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     1141 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg
     1201 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     1261 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
     1321 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     1381 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     1441 ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     1501 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     1561 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     1621 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     1681 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     1741 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     1801 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     1861 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     1921 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     1981 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     2041 gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa
     2101 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2161 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2221 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
