LOCUS       Exported                2794 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local.rgkiwi.48-7oueryg
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 96 documents from 
            Done.gb [pL0-T] Rb Glob pA (T1)(from pL0-T_Rb-Glob). 
            ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(4..9)
                     /label=BBb scar
     primer_bind     689..708
                     /label=G00101
     misc_feature    830..835
                     /label=BsaI
     protein_bind    830..835
                     /label=BsaI Bind
     misc_feature    837..840
                     /label=pL0 3-T
                     /label=nonstandard type:  gg_overhang
     terminator      841..1381
                     /label=Rb Glob Terminator (T1)
     misc_feature    842..847
                     /label=EcoRI
     polyA_signal    990..995
                     /label=Poly-A Signal
     polyA_signal    complement(1001..1006)
                     /label=Poly-A Signal
     misc_feature    1382..1385
                     /label=pL0 T
                     /label=nonstandard type:  gg_overhang
     misc_feature    complement(1387..1392)
                     /label=BsaI
     protein_bind    complement(1387..1392)
                     /label=BsaI Bind
     misc_feature    1542..1565
                     /label=Pamp
     misc_feature    complement(1557..1577)
                     /label=JH856R
     CDS             1612..2624
                     /label=Sm/Sp no DraIII
     misc_feature    2312..2317
                     /label=BBb scar
     misc_feature    2689..2694
                     /label=BBb scar
     misc_feature    complement(2701..2706)
                     /label=BBb scar
     rep_origin      complement(join(2719..2794,1..607))
                     /direction=LEFT
                     /label=ColE1 origin
     misc_feature    2787..2792
                     /label=BBb scar
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcacaac
      841 tgaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
      901 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
      961 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     1021 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     1081 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     1141 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     1201 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     1261 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     1321 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     1381 gcgcttgaga ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat acgcctattt
     1441 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     1501 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1561 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgcgctca
     1621 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     1681 atggcttgtt atgactgttt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     1741 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     1801 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat
     1861 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     1921 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc gatattgatt
     1981 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     2041 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     2101 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     2161 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     2221 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     2281 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     2341 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     2401 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     2461 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     2521 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     2581 tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca gaccaagttt
     2641 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2701 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2761 cgtcagaccc cgtagaaaag atcaaaggat cttc
//
