LOCUS       Exported                3213 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local.jones.3z-96us9fy
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 3 documents from 6 
            pL1s.gb pL1 Backbone, Gibson Seqs 1-2, SapI Seqs T1-2 [AmpR, RFP, 
            BsaI]. ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..683
                     /label=ColE1 origin
     primer_bind     complement(36..55)
                     /label=KK_SeqR_SeqPrimer
     misc_feature    272..277
                     /label=ApaLI
     misc_feature    369..377
                     /label=AlwNI
     misc_feature    460
                     /label=(g->a)
     CDS             complement(778..1638)
                     /label=AmpR
     primer_bind     1469..1489
                     /label=JH1030F
     misc_feature    1518..1523
                     /label=ApaLI
     misc_feature    complement(1639..1652)
                     /label=RBS
                     /label=nonstandard type:  motif
     misc_feature    complement(1680..1708)
                     /label=pAmp
     primer_bind     1736..1758
                     /label=KK_lvl1b_F66
     misc_feature    1747..1750
                     /label=Ins 7
                     /label=nonstandard type:  Gg_overhang
     primer_bind     1762..1791
                     /label=KK_lvl1_f60
     misc_feature    complement(1764..1781)
                     /label=I-SceI
     primer_bind     1764..1777
                     /label=KK_lvl1_r61
     misc_feature    1785..1829
                     /label=pL1 S1
                     /label=nonstandard type:  Gibson_Overhang
     misc_feature    complement(1805..1829)
                     /label=RJ_00429Ra
     protein_bind    1830..1836
                     /label=SapI Bind
     misc_feature    1830..1836
                     /label=SapI
     misc_feature    1838..1840
                     /label=pL1 T1
                     /label=nonstandard type:  Gg_overhang
     misc_feature    1841..1844
                     /label=pL0 I
                     /label=nonstandard type:  Gg_overhang
     primer_bind     1844..1863
                     /label=RJ_00430Fa
     misc_feature    1844..1858
                     /label=Infusion
                     /label=nonstandard type:  Gibson_Overhang
     protein_bind    complement(1846..1851)
                     /label=BsaI Bind
     misc_feature    complement(1846..1851)
                     /label=BsaI
     primer_bind     1852..1873
                     /label=ca998
     misc_feature    1913..1918
                     /label=EcoRI
     misc_feature    1928..1933
                     /label=XbaI
     misc_feature    1993..2014
                     /label=RBS
                     /label=nonstandard type:  motif
     CDS             2015..2692
                     /label=RFP
     misc_feature    2027..2032
                     /label=BbsI
     misc_feature    2312..2317
                     /label=BbsI
     misc_feature    2433..2438
                     /label=NcoI
     misc_feature    2471..2476
                     /label=BbsI
     misc_feature    2630..2635
                     /label=BbsI
     rRNA            2728..2808
                     /label=5S rRNA
     misc_feature    2859..2864
                     /label=SpeI
     misc_feature    2866..2871
                     /label=BamHI
     misc_feature    complement(2931..2950)
                     /label=JG_1.80
     misc_feature    complement(2944..2963)
                     /label=G00101
     primer_bind     complement(2944..2963)
                     /label=KK_G00101
     misc_feature    complement(2951..2971)
                     /label=RJ_00430Ra
     misc_feature    2957..2971
                     /label=Infusion
                     /label=nonstandard type:  Gibson_Overhang
     protein_bind    2964..2969
                     /label=BsaI Bind
     misc_feature    2964..2969
                     /label=BsaI
     misc_feature    2971..2974
                     /label=pL0 T
                     /label=nonstandard type:  Gg_overhang
     misc_feature    2975..2977
                     /label=pL1 T2
                     /label=nonstandard type:  Gg_overhang
     misc_feature    complement(2979..2985)
                     /label=SapI
     protein_bind    complement(2979..2985)
                     /label=SapI Bind
     misc_feature    2986..3030
                     /label=pL1 S2
                     /label=nonstandard type:  Gibson_Overhang
     primer_bind     2986..3018
                     /label=RJ_00429Fa
     misc_feature    3039..3056
                     /label=I-SceI
     primer_bind     complement(3043..3076)
                     /label=KK_lvl1_r61
     misc_feature    3067..3070
                     /label=Ins 1
                     /label=nonstandard type:  Gg_overhang
     rep_origin      3077..3186
                     /label=RK2 trfa
     misc_feature    complement(3183..3202)
                     /label=JH1030R
ORIGIN
        1 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
       61 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      121 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      181 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      241 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      301 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      361 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      421 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
      481 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      541 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      601 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      661 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
      721 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
      781 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      841 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      901 tgctgcaatg ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca
      961 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1021 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     1081 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     1141 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     1201 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     1261 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     1321 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     1381 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     1441 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     1501 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     1561 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     1621 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcaaggtta
     1681 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1741 gcgcacgaat tggccagcgc tgcattaccc tgttatccct aaccggttta ccgagctctt
     1801 attggttttc aaacttcatt gactgtgccg ctcttcaacc ggagtgagac cgtatcacga
     1861 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc
     1921 ggccgcttct agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata
     1981 ctgagcacta ctagagaaag aggagaaata ctagatggct tcctccgaag acgttatcaa
     2041 agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat
     2101 cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac
     2161 caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc
     2221 caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga
     2281 aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca
     2341 ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt
     2401 cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg
     2461 tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga
     2521 cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct
     2581 gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac
     2641 catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg
     2701 atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     2761 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     2821 actggctcac cttcgggtgg gcctttctgc gtttatatac tagtaggatc ctaactcgac
     2881 gtgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2941 tcagctcact caaaggcggt aatggtctca cgctcgctga agagcggtgc gtttttatgc
     3001 ttgtagtatt gtataatgtt tttaagatcc ttaattaata gggataacag ggtaatatct
     3061 tgccctgcac gaataccagc gaccccttgc ccaaatactt gccgtgggcc tcggcctgag
     3121 agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc
     3181 gccacatcta ggatctgcca ggaaccgtaa aaa
//
