LOCUS       Exported                3213 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local.jones.41-96us9g1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 3 documents from 6 
            pL1s.gb pL1 Backbone, Gibson Seqs 3-4, SapI Seqs T3-X [AmpR, RFP, 
            BsaI]. ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     674..694
                     /label=JH1030F
     misc_feature    723..728
                     /label=ApaLI
     misc_feature    complement(844..857)
                     /label=RBS
                     /label=nonstandard type:  motif
     primer_bind     878..898
                     /label=JH856R
     misc_feature    complement(885..913)
                     /label=pAmp
     primer_bind     941..963
                     /label=KK_lvl1b_F66
     misc_feature    complement(969..986)
                     /label=I-SceI
     primer_bind     969..982
                     /label=KK_lvl1_r61
     misc_feature    990..1034
                     /label=pL1 S3
                     /label=nonstandard type:  Gibson_Overhang
     misc_feature    complement(1009..1034)
                     /label=RJ_00429Re
     protein_bind    1035..1041
                     /label=SapI Bind
     misc_feature    1035..1041
                     /label=SapI
     misc_feature    1043..1045
                     /label=pL1 T3
                     /label=nonstandard type:  Gg_overhang
     misc_feature    1046..1049
                     /label=pL0 I
                     /label=nonstandard type:  Gg_overhang
     primer_bind     1049..1068
                     /label=RJ_00430Fa
     misc_feature    1049..1063
                     /label=Infusion
                     /label=nonstandard type:  Gibson_Overhang
     misc_feature    complement(1051..1056)
                     /label=BsaI
     protein_bind    complement(1051..1056)
                     /label=BsaI Bind
     primer_bind     1057..1078
                     /label=ca998
     misc_feature    1118..1123
                     /label=EcoRI
     misc_feature    1133..1138
                     /label=XbaI
     misc_feature    1198..1219
                     /label=RBS
                     /label=nonstandard type:  motif
     CDS             1220..1897
                     /label=RFP
     misc_feature    1232..1237
                     /label=BbsI
     misc_feature    1517..1522
                     /label=BbsI
     misc_feature    1638..1643
                     /label=NcoI
     misc_feature    1676..1681
                     /label=BbsI
     misc_feature    1835..1840
                     /label=BbsI
     rRNA            1933..2013
                     /label=5S rRNA
     misc_feature    2064..2069
                     /label=SpeI
     misc_feature    2071..2076
                     /label=BamHI
     misc_feature    complement(2136..2155)
                     /label=JG_1.80
     misc_feature    complement(2149..2168)
                     /label=G00101
     primer_bind     complement(2149..2168)
                     /label=KK_G00101
     misc_feature    complement(2156..2176)
                     /label=RJ_00430Ra
     misc_feature    2162..2176
                     /label=Infusion
                     /label=nonstandard type:  Gibson_Overhang
     misc_feature    2169..2174
                     /label=BsaI
     protein_bind    2169..2174
                     /label=BsaI Bind
     misc_feature    2176..2179
                     /label=pL0 T
                     /label=nonstandard type:  Gg_overhang
     misc_feature    2180..2182
                     /label=pL1 TX
                     /label=nonstandard type:  Gg_overhang
     misc_feature    complement(2184..2190)
                     /label=SapI
     protein_bind    complement(2184..2190)
                     /label=SapI Bind
     misc_feature    2191..2235
                     /label=pL1 S4
                     /label=nonstandard type:  Gibson_Overhang
     primer_bind     2191..2218
                     /label=RJ_00429Fe
     misc_feature    2194..2199
                     /label=EcoRI
     misc_feature    2244..2261
                     /label=I-SceI
     primer_bind     complement(2248..2281)
                     /label=KK_lvl1_r61
     rep_origin      2282..2391
                     /label=RK2 trfa
     misc_feature    complement(2388..2407)
                     /label=JH1030R
     rep_origin      2419..3101
                     /label=ColE1 origin
     primer_bind     complement(2454..2473)
                     /label=KK_SeqR_SeqPrimer
     misc_feature    2690..2695
                     /label=ApaLI
     misc_feature    2787..2795
                     /label=AlwNI
     CDS             complement(join(3196..3213,1..843))
                     /label=AmpR
ORIGIN
        1 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
       61 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      121 gcgagaacca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      181 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      241 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      301 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      361 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      421 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      481 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      541 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      601 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      661 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      721 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      781 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      841 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
      901 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca cgaattggcc
      961 agcgctgcat taccctgtta tccctaaccg gtctaatacc caatctctcg tcttatccag
     1021 atgttttata cgccgctctt catacggagt gagaccgtat cacgaggaag aatttcagat
     1081 aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagagt
     1141 ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag cactactaga
     1201 gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt
     1261 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga
     1321 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
     1381 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa
     1441 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga
     1501 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca
     1561 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc
     1621 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga
     1681 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga
     1741 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa
     1801 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta
     1861 cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag
     1921 atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1981 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     2041 ggtgggcctt tctgcgttta tatactagta ggatcctaac tcgacgtgca ggcttcctcg
     2101 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2161 gcggtaatgg tctcacgcta gatgaagagc ggtgaattcc cttatgtgag tgtaaaaggc
     2221 aggcgagttt gtcccttaat taatagggat aacagggtaa tatcttgccc tgcacgaata
     2281 ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc ctgagagcca aaacacttga
     2341 tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc gttgcgccac atctaggatc
     2401 tgccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2461 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2521 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2581 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2641 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2701 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2761 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2821 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     2881 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2941 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3001 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3061 tcagtagaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3121 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3181 aacttggtct gacagttacc aatgcttaat cag
//
