LOCUS       Exported                3191 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local.rgkiwi.a3-7wnb8up
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 96 documents from 
            Done.gb Level 1 Backbone, Seq 4 - Seq 5. Amp Resistance. RFP for R/W
            Screening(from pL1 [AmpR, RFP, S4-S5]). ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    137..181
                     /label=pL1 S4
                     /label=nonstandard type:  Gibson_Overhang
     misc_feature    140..145
                     /label=EcoRI
     primer_bind     182..202
                     /label=RJ_00069Fa
     misc_feature    complement(326..355)
                     /label=RJ_00069Ra
     CDS             356..1033
                     /label=RFP
     primer_bind     1034..1059
                     /label=RJ_00069Fa
     rRNA            1069..1149
                     /label=5S rRNA
     misc_feature    complement(1297..1315)
                     /label=RJ_00069Ra
     misc_feature    1316..1360
                     /label=pL1 S5
                     /label=nonstandard type:  Gibson_Overhang
     rep_origin      1407..1516
                     /label=RK2 trfa
     misc_feature    complement(1513..1532)
                     /label=JH1030R
     misc_feature    1521..1526
                     /label=BBb scar
     rep_origin      complement(1544..2226)
                     /direction=LEFT
                     /label=ColE1 origin
     misc_feature    2142..2147
                     /label=BBb scar
     misc_feature    complement(2153..2158)
                     /label=BBb scar
     misc_feature    2239..2244
                     /label=BBb scar
     misc_feature    complement(2251..2256)
                     /label=BBb scar
     CDS             2324..3181
                     /label=AmpR
     primer_bind     3012..3032
                     /label=JH1030F
     misc_feature    3019..3024
                     /label=BBb scar
     misc_feature    complement(3036..3041)
                     /label=BBb scar
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtt ccgcgcacga attggccagc gctgcattac
      121 cctgttatcc ctaaccggtg aattccctta tgtgagtgta aaaggcaggc gagtttgtcc
      181 cggagtgaga ccgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta
      241 aggatgattt ctggaattcg cggccgcttc tagagtccct atcagtgata gagattgaca
      301 tccctatcag tgatagagat actgagcact actagagaaa gaggagaaat actagatggc
      361 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
      421 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
      481 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
      541 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
      601 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg
      661 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
      721 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
      781 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa
      841 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
      901 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
      961 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
     1021 caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa
     1081 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1141 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     1201 ctagtaggat cctaactcga cgtgcaggct tcctcgctca ctgactcgct gcgctcggtc
     1261 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatggtctc acgctggttg
     1321 cttgcaaaag cagtaattgg aaagcactct caaagaatcc ttaattaata gggataacag
     1381 ggtaatatct tgccctgcac gaataccagc gaccccttgc ccaaatactt gccgtgggcc
     1441 tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg
     1501 gcatcgttgc gccacatcta ggatctgcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1561 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1621 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1681 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1741 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1801 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1861 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1921 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1981 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     2041 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2101 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2161 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2221 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2281 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2341 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2401 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2461 gagaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2521 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2581 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2641 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2701 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2761 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2821 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2881 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2941 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3001 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagatcgatg taacccactc
     3061 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3121 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3181 tactcttcct t
//
