LOCUS       Exported                2110 bp ds-DNA     circular SYN 29-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local.rgkiwi.a6-7wnb8uq
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 96 documents from 
            Done.gb Level 1 Backbone, Seq 1 - Seq 2. Amp Resistance. RFP for R/W
            Screening(pL1 [AmpR, RFP, S1-S2]). ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(3..8)
                     /label=BBb scar
     misc_feature    89..94
                     /label=BBb scar
     misc_feature    complement(101..106)
                     /label=BBb scar
     CDS             174..1031
                     /label=AmpR
     primer_bind     862..882
                     /label=JH1030F
     misc_feature    869..874
                     /label=BBb scar
     misc_feature    complement(886..891)
                     /label=BBb scar
     misc_feature    complement(1120..1139)
                     /label=RJ_00139Rb
     primer_bind     1139..1156
                     /label=RJ_00139Fa
     misc_feature    1140..1143
                     /label=Ins 7
                     /label=nonstandard type:  gg_overhang
     misc_feature    complement(1157..1174)
                     /label=I-SceI
     misc_feature    1178..1222
                     /label=pL1 S2
                     /label=nonstandard type:  Gibson_Overhang
     misc_feature    1223..1226
                     /label=Q
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    1227..1233
                     /label=SapI
     protein_bind    1227..1233
                     /label=SapI Bind
     misc_feature    1235..1237
                     /label=pL1 T2
                     /label=nonstandard type:  gg_overhang
     misc_feature    1238..1252
                     /label=Infusion
                     /label=nonstandard type:  gibson_overhang
     misc_feature    1238..1244
                     /label=fr Nluc
     misc_feature    complement(1245..1253)
                     /label=fr Nluc
     misc_feature    1254..1260
                     /label=fr Nluc
     misc_feature    1261..1263
                     /label=pL1 TX
                     /label=nonstandard type:  gg_overhang
     misc_feature    complement(1265..1271)
                     /label=SapI
     protein_bind    complement(1265..1271)
                     /label=SapI Bind
     misc_feature    1272..1275
                     /label=Q
     misc_feature    1276..1320
                     /label=pL1 S3
                     /label=nonstandard type:  Gibson_Overhang
     misc_feature    1329..1346
                     /label=I-SceI
     misc_feature    complement(1340..1361)
                     /label=RJ_00139Ra
     misc_feature    1357..1360
                     /label=Ins 1
                     /label=nonstandard type:  gg_overhang
     primer_bind     1361..1380
                     /label=RJ_00139Fb
     rep_origin      1367..1476
                     /label=RK2 trfa
     misc_feature    complement(1473..1492)
                     /label=JH1030R
     misc_feature    1481..1486
                     /label=BBb scar
     rep_origin      complement(join(1504..2110,1..76))
                     /direction=LEFT
                     /label=ColE1 origin
     misc_feature    2102..2107
                     /label=BBb scar
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaagg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcacg
     1141 aattggccag cgctgcatta ccctgttatc cctaaccggt gcgtttttat gcttgtagta
     1201 ttgtataatg tttttaagat ccggaggctc ttcacgcgac catcggttga tcagttcgac
     1261 agatgaagag ccgctggtct aatacccaat ctctcgtctt atccagatgt tttatacgcc
     1321 ttaattaata gggataacag ggtaatatct tgccctgcac gaataccagc gaccccttgc
     1381 ccaaatactt gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg
     1441 tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta ggatctgcca ggaaccgtaa
     1501 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1561 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1621 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1681 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1741 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1801 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1861 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1921 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1981 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2041 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2101 aggatctcaa
//
